Diversity in the carotenoid profiles and the expression of genes related to carotenoid accumulation among citrus genotypes     

Yoshinori Ikoma  Hikaru Matsumoto  Masaya Kato 《Breeding Science》2016,66(1):139-147

Carotenoids are not only important to the plants themselves but also are beneficial to human health. Since citrus fruit is a good source of carotenoids for the human diet, it is important to study carotenoid profiles and the accumulation mechanism in citrus fruit. Thus, in the present paper, we describe the diversity in the carotenoid profiles of fruit among citrus genotypes. In regard to carotenoids, such as β-cryptoxanthin, violaxanthin, lycopene, and β-citraurin, the relationship between the carotenoid profile and the expression of carotenoid-biosynthetic genes is discussed. Finally, recent results of quantitative trait locus (QTL) analyses of carotenoid contents and expression levels of carotenoid-biosynthetic genes in citrus fruit are shown.  相似文献   

胡萝卜根中主要类胡萝卜素含量相关QTL的精细定位     

欧承刚  孙婷婷  刘新艳  李成江  徐东辉  赵志伟  庄飞云 《园艺学报》2017,44(2):288-296

以白色的野生胡萝卜‘松滋野生’(Ws)和橘色的栽培胡萝卜品种‘Amsterdam’(Af)为亲本构建的回交重组自交系(BIL)为试材,基于低倍重测序技术开发SNP标记,构建了由1 976个Bin标记组成,包含29 435个SNP标记的遗传图谱。图谱总距离834.28 c M,平均图距0.42 c M。通过对胡萝卜肉质根中类胡萝卜素含量相关QTL分析,在连锁群LG04和LG08中检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、叶黄素、玉米黄质和总类胡萝卜素含量的主效QTL(M-QTL)2、2、3、2、2和2个,表型贡献率为11.47%~19.18%;另检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、玉米黄质和总类胡萝卜素含量的上位性QTL(E-QTL)1、1、2、1和1个,表型贡献率为2.50%~3.66%。在M-QTL显著区间内共检索到36个有功能注释的预测基因,其中Dck018297为ζ–胡萝卜素脱氢酶2基因,与调控β–胡萝卜素合成和总类胡萝卜素含量有关;Dck008006为乙烯响应因子2.2的同源基因,与调控α–胡萝卜素、ζ–胡萝卜素合成有关;Dck029898为转录因子b HLH135的同源基因,与调控玉米黄质合成有关。  相似文献   

植物叶色变化机制研究进展   总被引：3，自引：0，他引：3  

李卫星  杨舜博  何智冲  金飚 《园艺学报》2017,44(9):1811-1824

介绍了叶色变化的生态适应意义、环境条件影响、生理生化以及叶绿素、类胡萝卜素、花青素合成代谢相关基因转录和mi RNA调控等方面的研究进展,为植物色素研究和叶色品种改良提供参考。  相似文献   

Screening of Human CYP1A2 and CYP3A4 Inhibitors from Seaweed In Silico and In Vitro     

Sung-Kun Yim  Kian Kim  SangHo Chun  TaeHawn Oh  WooHuk Jung  KyooJin Jung  Chul-Ho Yun 《Marine drugs》2020,18(12)

Phenolic compounds and carotenoids are potential inhibitors of cytochrome P450s. Sixteen known compounds, phenolic compounds and carotenoids from seaweed were examined for potential inhibitory capacity against CYP1A2 and CYP3A4 in silico and in vitro. Morin, quercetin, and fucoxanthin inhibited the enzyme activity of CYP1A2 and CYP3A4 in a dose-dependent manner. The IC₅₀ values of morin, quercetin, and fucoxanthin were 41.8, 22.5, and 30.3 μM for CYP1A2 and 86.6, 16.1, and 24.4 μM for CYP3A4, respectively. Siphonaxanthin and hesperidin did not show any significant effect on CYP1A2, but they slightly inhibited CYP3A4 activity at high concentrations. In silico modeling of CYP’s binding site revealed that the potential inhibitors bound in the cavity located above the distal surface of the heme prosthetic group through the 2a or 2f channel of CYPs. This study presents an approach for quickly predicting CYP inhibitory activity and shows the potential interactions of compounds and CYPs through in silico modeling.  相似文献   

Evaluation of Intestinal Absorption of Dietary Halocynthiaxanthin,a Carotenoid from the Sea Squirt Halocynthia roretzi     

Chiaki Ikeda  Yuki Manabe  Nami Tomonaga  Tatsuya Wada  Takashi Maoka  Tatsuya Sugawara 《Marine drugs》2020,18(12)

Halocynthiaxanthin is an acetylenic carotenoid mainly found in Halocynthia roretzi. To date, several bioactivities of halocynthiaxanthin have been reported, but its mechanism of digestion and absorption in mammals has not been studied yet. In this study, we evaluated the intestinal absorption of halocynthiaxanthin in mice. The halocynthiaxanthin-rich fraction was prepared from the tunicate Halocynthia roretzi. Mice were orally administered the fraction at a dose of 5 mg/kg body weight. The halocynthiaxanthin levels in the plasma, liver, and small intestine, were quantified using HPLC-PDA, 1, 3, 6, and 9 h after ingestion. The halocynthiaxanthin-rich fraction mainly consisted of the all-trans form and a small amount of cis forms. These three isomers were detected in the plasma of mice 3 h after ingestion. Time-course changes after the ingestion of this fraction were found, with cis isomers being more abundant than the all-trans isomer in the mouse plasma and liver. In the small intestine, however, the all-trans isomer was primarily detected. The possibility that cis isomers might be absorbed rapidly from the small intestine cannot be denied, but our results suggest that dietary all-trans-halocynthiaxanthin might be isomerized to the cis isomer after intestinal absorption.  相似文献   

不同产区烤烟中质体色素及降解产物的研究   总被引：46，自引：0，他引：46  

杨虹琦  周冀衡  罗泽民  杨述元 《西南大学学报(自然科学版)》2004,26(5):640-644

探索不同生态环境与烤烟中质体色素及其相关降解产物的关系,以及质体色素及其降解产物与烟草香型、香气质和香气量的关系,以我国不同生态条件的烤烟产区烟叶为材料,对烤烟样品中的叶绿素、类胡萝卜素及其相关降解产物的含量进行了分析。研究结果表明,云南、贵州、重庆等产区烤烟中的类胡萝卜素含量普遍高于东北和福建等烤烟产区;不同烤烟产区之间类胡萝卜素降解产物的含量存在很大差异;并且云南烤烟独特香气风格的形成与其烟叶中各种质体色素降解产物的协调性有关。  相似文献   

榉树叶色和色素组成的相互关系研究     

张亚平  曾艳  林雪莹  金晓玲  刘晓玲 《河南农业科学》2017,46(6)

探析红色、黄色、绿色3种不同色系榉树叶色形成的化学机制,为选育、培育榉树优良株系提供理论依据。以秋季转色期叶色表现为红色、黄色、绿色的榉树叶片为材料,分析其叶片的颜色参数及花青素、叶绿素、类胡萝卜素等色素含量的变化特点,并分析叶色与色素组成间的相互关系。结果表明:秋季叶片转色期,红色系榉树的色相a~*值最高,在10月25日达到最大值,呈现出最佳观赏效果;绿色系榉树a~*值较低,且变化趋势相对平缓;在转色后期,黄色系榉树的b~~*值显著大于红色和绿色系榉树,但其在秋季变色期存在返绿现象。明度L~*值、彩度C~*值与色相b~*值的变化趋势几乎完全一致。不同色系榉树都含有花青素、叶绿素和类胡萝卜素,但不同色系榉树3种色素的含量不同。红色系榉树花青素含量百分比最高,为22.1%~44.6%;绿色系榉树的总叶绿素含量最高,为73.0%~80.5%;黄色系榉树的类胡萝卜素含量最高,为24.7%~35.4%。回归分析结果表明,榉树叶片中,花青素和叶绿素含量与榉树的叶片呈色密切相关。其中花青素含量与a~*值呈正相关,与L~*、b~*、C~*呈负相关,说明花青素含量的增加,使叶片红色度增加,同时明度、黄色度和彩度降低;叶绿素含量与L~*、a~*、b~*、C~*均呈负相关,说明叶绿素含量的增加,使叶片的明度、红色度、黄色度以及彩度均降低;类胡萝卜素含量未被引入方程,说明类胡萝卜素含量与叶片颜色参数无显著相关性(P0.05)。分析显示,花青素含量增加,叶绿素含量降低,可以使叶片变红;而花青素含量降低,叶绿素含量增加,叶片将维持绿色;若花青素与叶绿素同时降低,则将使叶片变黄。叶片内叶绿素、类胡萝卜素、花青素的含量及百分比是榉树呈现红色、黄色、绿色3种不同色系的物质基础和根本原因。  相似文献   

不同烤烟品种成熟及烘烤过程中类胡萝卜素含量分析     

杨虹琦  陈若星  张发明  李永智  白柯  周冀衡 《云南农业大学学报(自然科学版)》2012,27(3):379-384

 以K326、云烟85和红花大金元中部烟叶为材料,分析了相同生态条件下不同烤烟品种成熟及烘烤过程中烟叶类胡萝卜素含量的变化。结果表明：在烟叶成熟期和烘烤过程中,不同烤烟品种类胡萝卜素含量及成熟期降解速度均存在很大差异。K326欠熟烟叶中类胡萝卜素含量很高,但是随着叶片衰老变黄,类胡萝卜素含量大幅、快速降低。云烟85和红花大金元欠熟烟叶中类胡萝卜素含量明显低于K326,但红花大金元成熟期类胡萝卜素的降解幅度明显小于K326和云烟85。因此,红花大金元适熟和过熟烟叶中的类胡萝卜素含量均高于K326和云烟85。烘烤过程中3个烤烟品种烟叶的类胡萝卜素含量均呈现下降趋势,但以烘烤温度由25℃升至45℃的变黄期下降幅度最大,并且也呈现K326降解速度最快,红花大金元最慢的变化规律,因此,红花大金元初烤烟叶中的类胡萝卜素含量高于K326和烟85。  相似文献   

高压脉冲电场法提取耐辐射奇球菌中类胡萝卜素的研究     

何宇超  程琪琪  吴莉  张莉莉  范成凯  应南娇  汪婷  杨勇 《核农学报》2020,34(2):315-321

为探讨高压脉冲电场(PEF)电学参数致耐辐射奇球菌破壁提取类胡萝卜素的影响及最佳工艺参数,采用正交试验法,分析了电场强度、处理时间、频率和脉宽4种电场参数对类胡萝卜素提取效果的影响。结果表明,PEF技术提取类胡萝卜素的最优提取水平组合为:电场强度30 kV·cm^-1,处理时间30 s,频率30 Hz,脉宽10 μs。该条件下,提取量为75.06 μg·g^-1,与未进行电场处理的样品提取量48.27 μg·g^-1相比,增加了55.5%。本研究结果为PEF技术的工业化大规模应用提供了理论依据。  相似文献   

盐胁迫对黄瓜幼苗叶片光合色素含量的影响   总被引：34，自引：0，他引：34  

王素平  郭世荣  胡晓辉  李璟  焦彦生 《江西农业大学学报》2006,28(1):32-38

采用营养液水培,选用耐盐性不同的黄瓜品种“长春密刺”和“津春2号”,研究了不同浓度(0、25、50、75、100 mmol/L)NaC l处理对黄瓜幼苗植株功能叶片、下位叶片中叶绿素(chl)含量和类胡萝卜素(car)含量的影响。结果表明,在盐胁迫下,黄瓜幼苗功能叶片中chla、chlb、chl、car含量在盐胁迫1 d后开始升高,3 d、5 d时达到最大值,且含量随着NaC l浓度的增加而增加,7 d、9 d时下降;“长春密刺”chl和car含量升高较早,但升高幅度明显低于“津春2号;”car/chl值下降,且“津春2号”下降幅度明显大于“长春密刺”。盐胁迫下,黄瓜幼苗下位叶片中chl总量和car含量下降幅度明显大于对照。  相似文献