Decreased transport of orally administered protein into the blood circulation of developing juveniles of Japanese eel Anguilla japonica     

Osamu Nakamura  Yuzuru Suzuki  Katsumi Aida  Hajime Hatta 《Fisheries Science》2001,67(5):863-869

ABSTRACT: To clarify the quantitative changes in the transport of orally intubated protein into the blood circulation as macromolecules in development, immunoglobulin Y (IgY) extracted from chicken eggs was administered orally to juvenile Japanese eel, Anguilla japonica . For the first experiment, which was performed before the commencement of artificial feeding, the oral delivery of 2.0 μg/0.1 g bodyweight of IgY resulted in a rapid increase in plasma IgY to a maximum of 2.30 μg/mL. However, the transport of IgY into the blood decreased significantly in the experiments that followed, which were performed after 12, 25 and 42 days. During this period, bodyweight increased approximately by a factor of eight, and rapid growth of the stomach was observed histologically. Possible contributions for the development of the alimentary canal to the diminishment of intestinal protein assimilation are discussed.  相似文献   

Patterns of silver eel (Anguilla anguilla L.) sex ratio in a catchment     

P. Laffaille  A. Acou  J. Guillouët  B. Mounaix  A. Legault 《Ecology of Freshwater Fish》2006,15(4):583-588

Abstract –  Changes in the numbers and size-class structure of European silver eels, Anguilla anguilla , in the River Frémur (France) were examined over a 9-year period after installation of downstream eel passes. The number of silver eels migrating downstream peaked in 1999, then decreased strongly and steadily after 2000, reaching relatively low levels. At the same time, a gradual shift in the silver eel sex ratio from a dominance of males (size from 270 to 442 mm, age from 3 to 6 years) to females (size from 366 to 1112 mm, age from 4 to 9 years) was recorded. Possible explanations for the escapement patterns observed are environmental sex determination and the installation of eel passes on the main hydraulic engineering structures in 1992 and 1996.  相似文献   

黄鳝排尿器官的解剖学研究     

曾增  刘希彬  吴峻岩  李琦  谢从新  杨春 《华中农业大学学报》2002,21(1):72-76

应用铸型技术等方法研究了黄鳝排尿器官的解剖学结构。结果见到,黄鳝2条中肾管在中肾末端与所谓退化性腺（亦即膀胱）相连,并与该“退化性腺”之间存在着明显的通道关系,而“退化性腺”直接通于尿孔。此外,组织学的研究也支持了解剖学结果,并证实中肾管在“退化性腺”中存在着2种开口类型;单口型和双口型。研究结果为作者先前提出的有关黄鳝“退化性腺”是膀胱的结论提供了直接的形态学证据。  相似文献   

Effects of silvering state on induced maturation and spawning in wild female Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis>     

Akihiro Okamura  Yoshiaki Yamada  Noriyuki Horie  Tomoko Utoh  Naomi Mikawa  Satoru Tanaka  Katsumi Tsukamoto 《Fisheries Science》2008,74(3):642-648

ABSTRACT:   The effects of silvering state of wild female Japanese eels Anguilla japonica on the success of induced maturation and the following spawning were examined. Thirty-eight females, collected in Mikawa Bay, were divided into four stages based on their silvering state: yellow (Y1), late-yellow (Y2), silver (S1) and late silver eels (S2). Despite injections of salmon pituitary extract (SPE) through the standard technique, Y1 and Y2 eels did not respond to the treatment with undeveloped gonad (gonad-somatic index [GSI]: 0.3–0.9), and all these females died by 5 weeks, probably due to an abnormal physiological condition. Most S1 (81%) and S2 eels (100%) matured completely (GSI: 17.8–51.4), and finally spawned successfully (69% for S1, 89% for S2). S2 eels fully matured with oocytes of over 750 μm in diameter by significantly smaller number of injections of SPE (5–6 times) than the case of S1 eels (6–8 times). The amount of eggs released by S2 eels (0.65 ± 0.11 g/fish per body weight [BW]) was significantly larger than those by S1 eels (0.54 ± 0.09 g/fish per BW). There was no difference in fertilization and hatching rates between eggs released by S1 eels and those of S2 eels. These results indicate that the success of induced maturation and spawning in wild female Japanese eels depends on their silvering state, and matured eggs can be obtained efficiently through the use of S2 eels rather than other stages.  相似文献   

Application of ELISA-based kit for detecting AOZ and determining its clearance in eel tissues     

Laddawan Krongpong  Kunihiko Futami  Takayuki Katagiri  Makoto Endo  Masashi Maita 《Fisheries Science》2008,74(5):1055-1061

Furazolidone, an antibacterial drug that was once widely used in the livestock industry and aquaculture, is now prohibited in numerous countries. It is difficult to detect residual furazolidone because it is readily metabolized in animal tissues but, by using and liquid chromatography coupled with tandem mass spectrometry, its metabolite, 3-amino-2-oxazolidinone (AOZ) can be detected. Here we describe the validity of an enzyme-linked immunosorbent assay (ELISA) kit to detect AOZ in Japanese eel Anguilla japonica tissue. ELISA is capable of detecting AOZ at 1.0 μg/kg in an eel sample with excellent accuracy and precision. Our results show that ELISA is suitable for regulatory purposes and for studying the fate of AOZ residues in eel treated with furazolidone. To measure the persistence of AOZ in eel tissues, eels (1.4–6.5g) were immersed in tanks containing 2 and 10 mg furazolidone/L for 3 h, and then maintained in a tank supplying well water for the next 160 days. The half-lives of AOZ, calculated from the linear terminal part of the excretion curve, were 25.0 days in muscle and 21.6 days in liver from fish exposed to 2 mg/L furazolidone. In the eels treated with 10 mg/L furazolidone, by contrast, high levels of AOZ were detected in liver and muscle, but the half-lives of AOZ were similar to those in fish treated with 2 mg/L furazolidone. The half-lives of AOZ in eel tissues were prolonged by the condition of low water temperature.  相似文献   

环境因子对黄鳝血清褪黑激素水平的影响   总被引：3，自引：0，他引：3  

石琼  颜远义  胡敏 《华中农业大学学报》2003,22(4):385-388

研究光周期和水温这 2种重要的环境因子对黄鳝血清褪黑激素水平的影响。结果表明 :1)连续光照可导致黄鳝血清褪黑激素水平的昼夜节律消失 ;连续黑暗环境中黄鳝血清褪黑激素水平仍保持昼夜节律性 ,但波动幅度变窄 ;光暗颠倒导致黄鳝血清褪黑激素水平出现 180度移位。从而说明黄鳝血清褪黑激素水平的相位变化基本能反映出光周期的时程改变。 2 )在一定的温度范围 (15～ 2 1℃ )内 ,黄鳝血清褪黑激素水平保持相对稳定 ;高温 (31℃ )则刺激黄鳝血清褪黑激素水平显著升高 (P <0 .0 0 1)。由此可见 ,黄鳝血清褪黑激素水平受到环境光周期与水温的协同调节。温度相关性的幅度变化协同光周期依赖性的时相变化 ,可能是调节褪黑激素发挥生殖调节作用的 2种重要方式。  相似文献   

孟加拉进口黄鳝中致病性嗜水气单胞菌的分离与鉴定     

王 昱袁增壮  聂福平等 《安徽农业科学》2013,(24):9997-9999,10026

[目的]了解致病性睹水气单胞菌的存在状况和风险水平.[方法]采用分离培养、生化鉴定、序列测定和动物试验等方法,对2批孟加拉进口黄鳝进行致病性睹水气单胞菌检测.[结果]从孟加拉进口黄鳝体内分离出4株高度致病性的嗜水气单胞菌.嗜水气单胞菌对孟加拉黄鳝本身致病性不明显,但是对小鼠的致病性却高达10 000～ 100 000 CFU/ml.[结论]孟加拉进口黄鳝存在致病性睹水气单胞菌的感染,且对我国的食品和卫生安全存在一定的风险.  相似文献   

患“红肝病”鳗鲡疱疹病毒的分离和鉴定     

卓玉琛 《福建农业学报》2015,(2):117-120

利用鳗鲡疱疹病毒(HVA)DNA聚合酶基因引物对患病双色鳗鲡和美洲鳗鲡进行PCR检测(PCR检测结果仅有双色鳗鲡),获得约394bp的目的条带,测序表明扩增出的条带为AHV的DNA聚合酶基因片段,与GeneBank公布的HQ992949、GU233800、FJ940765等6个鳗鲡疱疹病毒基因同源性为100%,与GU205167、AF363783鳗鲡疱疹病毒基因同源性为99%。将患病双色鳗鲡、美洲鳗鲡肝脏和肾脏组织的除菌滤液,接种EPC细胞,细胞7d出现CPE,双色鳗鲡株传代至15代,美洲鳗鲡株传代至5代。  相似文献   

黄鳝体长与血清胆固醇含量关系的分析     

张廷华  曾凡坤  李念  陈亮  田晓花 《湖北农业科学》2007,46(1):144-146

测定了不同体长黄鳝血清的胆固醇含量,发现黄鳝体长(x)与血清胆固醇含量(y)之间存在极显著的直线相关,回归方程y=6.059x 2.164,相关系数r=0.628 6.  相似文献   

微孔曝气增氧技术在鳗鲡养殖中的应用     

张哲  关瑞章  江兴龙  杨方圆 《厦门水产学院学报》2011,(5):335-339

比较研究了鳗鱼养殖中应用微孔曝气增氧与水车式增氧机增氧两种方式的增氧效果.结果表明：在未载鱼情况下,两种增氧方式的增氧能力具有极显著性差异（P〈0.01）,微孔曝气增氧方式比水车式增氧机增氧方式的单位水体增氧能力提高了15.85%,增氧动力效率是水车式增氧机增氧的2.36倍.在载鱼养殖情况下,使用微孔曝气增氧的试验池表层水的平均溶解氧值显著低于使用水车式增氧机增氧的值（P〈0.05）,但底层水的溶解氧两者没有显著差异（P〉0.05）,且溶解氧值都大于5 mg/L.微孔曝气增氧方式单位养殖水体的用电量比水车式增氧机增氧节省57.6%,且无安全隐患.由于微孔曝气增氧池水的流动性小,鱼类活动消耗的能量减少,且水温较高,因此,使用微孔曝气增氧方式的鳗鲡养殖效果较好.  相似文献