Tuberculosis vaccination sequence effect on protection in wild boar     

《Comparative immunology, microbiology and infectious diseases》2019

The Eurasian wild boar (Sus scrofa) is a reservoir for tuberculosis (TB) in which vaccination is a valuable tool for control. We evaluated the protection and immune response achieved by homologous and heterologous regimes administering BCG and heat-inactivated Mycobacterium bovis (IV). Twenty-one wild boar piglets were randomly allocated in five groups: Control, homologous BCG, homologous IV, heterologous IV-BCG, heterologous BCG-IV. Significant 67% and 66% total lesion score reductions were detected in homologous IV (IVx2) and heterologous IV-BCG groups when compared with Control group (F_4,16 = 6.393, p = 0.003; Bonferroni _{Control vs IVx2} p = 0.026, Tukey _{Control vs IV-BCG} p = 0.021). No significant differences were found for homologous BCG (although a 48% reduction in total lesion score was recorded) and BCG-IV (3% reduction). Heterologous regimes did not improve protection over homologous regimes in the wild boar model and showed variable results from no protection to similar protection as homologous regimes. Therefore, homologous regimes remain the best option to vaccinate wild boar against TB. Moreover, vaccine sequence dramatically influenced the outcome underlining the relevance of studying the effects of prior sensitization in the outcome of vaccination.  相似文献   

猪口蹄疫O型灭活疫苗PD50效检攻毒方式探索   总被引：2，自引：2，他引：0  

郎洪武  闫岩  张荣军  肖怀红  杨汉春 《中国兽药杂志》2004,38(3):26-27

用10 000 TCID50的OR/80株F13细胞毒或用10 000 LD50的OR/80株乳鼠组织毒(ORMF8),通过肌肉注射途径、蹄踵球部或趾间皮内注射途径接种O型口蹄疫抗体阴性架子猪进行攻毒试验,三种途径均不能使试验猪产生规律性发病.  相似文献   

论网络银行发展与金融监管的新问题     

张强 《湖南农业大学学报(自然科学版)》2001,(2)

银行业网络化的出现 ,已不再是一种单纯的金融创新 ,它将引发一场金融业、乃至整个经济局势的重大变革 ,这是历史赋予我们的一次绝好发展机遇 ,我们必须对这个问题引起高度重视。监管问题将是未来网络银行发展中一个十分重要的方面。目前我国网络银行发展水平较低 ,因此 ,必须以发展眼光来充分认识网络银行发展中监管面临的新问题 ,积极作好应对策略  相似文献   

1株鸭源非O1/O139群霍乱弧菌的分离鉴定与致病性分析     

张天天  吴征卓  陈国权  王娜  周碧君  程振涛  文明  王开功 《中国畜牧兽医》2021,48(4):1457-1465

为探明引起贵州省某鸭场雏鸭发病的病原及其致病性和耐药情况，本研究对该鸭场送的疑似细菌感染病鸭进行剖检，取鼻黏膜、心脏和肝脏等组织器官接种于培养基中进行细菌分离鉴定，通过对分离菌进行药敏试验、动物回归试验和毒力基因检测研究其耐药情况和致病性。结果显示，分离菌在血琼脂培养基上生长16 h后呈现为边缘整齐、有光泽的乳白色菌落，伴有β-溶血现象，经革兰氏染色后在生物显微镜下呈两端钝圆、弧状、排列无规则的革兰氏阴性短小杆菌，与霍乱弧菌相符；16S rDNA基因序列同源性及系统进化树显示，该分离菌与霍乱弧菌同源性高达99.6%~99.7%聚为一支；药敏试验结果显示，分离菌对大部分药物都表现为耐药，其中对氨苄西林、克林霉素、复方新诺明、苯唑西林和克林霉素等抗菌药耐药性较强，对头孢哌酮和头孢曲松敏感；动物回归试验显示，分离菌可导致试验组雏鸭5 d内全部发病死亡，表明该分离菌对雏鸭具有较强的致病性；毒力基因PCR检测结果显示，检测的霍乱弧菌相关毒力基因hlyA、ompW和chxA为阳性，而检测的O1群rfb、O139群rfb、tcpA及ctxA基因为阴性，表明本次分离的霍乱弧菌携带有致病基因，但不属于O1和O139血清群。结果表明，该鸭场雏鸭发病的疫情病原为非O1/O139血清群霍乱弧菌，该菌致病性强且对多种抗菌药物耐药。本试验结果为贵州省鸭霍乱弧菌病的防控提供了参考依据。  相似文献   

鱼肠道弧菌免疫检测方法的研究     

刘慧  邓灯  王忠  程顺峰  孙玉霞 《青岛农业大学学报(自然科学版)》2011,(3):223-226

采用灭活的鱼肠道弧菌作为抗原,制备兔抗血清,建立了鱼肠道弧菌的间接荧光抗体检测技术(indirect im-munofluorescence assay test,IFAT)、Western-blotting免疫印迹检测技术。IFAT结果显示阳性信号覆盖整个菌体,明亮,清晰,荧光信号呈长弧形且两端钝圆,与鱼肠道弧菌形状一致。Western-blotting结果显示共10条蛋白带发生免疫反应,其中6条蛋白带结果较清晰,显示出较强的特异性免疫反应,阴性对照组无条带。IFAT和Western-blotting结果说明所建立检测方法能够准确、快速的检测出鱼肠道弧菌。  相似文献   

新媒体时代公共图书馆创新服务研究——以重庆图书馆为例     

赵德菊 《农业网络信息》2015,(6)

随着新媒体时代的到来,人们接受信息的途径也有了全新的变化。新媒体给公共图书馆带来了机遇和挑战,公共图书馆面对种种挑战,积极创新服务,增强服务能力,提高服务水平和质量。  相似文献   

斑马鱼幼鱼嗜中性粒细胞对副溶血弧菌清除的动态变化     

郭欣娅  季策  谢子健  季帆  祖尧  任建峰  张庆华 《上海海洋大学学报》2020,29(2):180-188

副溶血弧菌(Vibrio parahaemolyticus)是人-兽-鱼共患的致病菌,严重威胁食品安全和人类的健康。为了研究宿主嗜中性粒细胞清除细菌感染的动态变化过程,利用红色荧光蛋白标记的副溶血弧菌Vp57~(RFP)菌株感染受精后48 h(hours post fertilization,hpf)的斑马鱼(Danio rerio)幼鱼耳泡,建立局部感染模型,并以大肠杆菌(Escherichia coli)Ec01菌株为对照,系统比较了副溶血弧菌和大肠杆菌的半数致死剂量(median lethal dose,LD_(50))、存活曲线以及和嗜中性粒细胞相互作用的动态过程,RT-qPCR验证炎症相关基因il1b和il10在感染前后的表达量变化。结果显示副溶血弧菌和大肠杆菌感染斑马鱼幼鱼的LD_(50)分别为7.90×10~8 CFU/mL和1.14×10~(11) CFU/mL,与感染大肠杆菌相比,斑马鱼感染副溶血弧菌后招募嗜中性粒细胞的速度更快,且数量更多。RT-qPCR结果发现斑马鱼感染副溶血弧菌后在相同时间点能够激活更高的il1b和il10基因的表达,引起强烈的炎症反应。基于以上研究,我们建立的副溶血弧菌-斑马鱼幼鱼耳泡局部感染模型,为进一步研究嗜中性粒细胞清除副溶血弧菌感染的动态过程,以及深入揭示天然免疫应答机制提供了依据。  相似文献   

北海近岸养殖海域3种弧菌的分离鉴定及耐药性分析     

陈盛峰  陈佳  禤金彩  龙寒  袁汉文  何秀苗 《动物医学进展》2016,(6):29-34

对北海近岸养殖海域的3种弧菌(Vibrio)进行了分离鉴定,并对鉴定的弧菌进行耐药性分析。通过从北海近岸养殖海域随机采集水样,利用TCBS培养基对所采水样进行弧菌的分离和纯化,采用PCR方法和序列分析对弧菌进行鉴定,并对鉴定出的3种弧菌进行了常用抗菌药物耐药性的检测。共分离获得67株疑似弧菌菌株,经鉴定,19株为副溶血性弧菌(Vibrio parahaemolyticus),4株为霍乱弧菌(Vibrio cholera),4株为河流弧菌(Vibrio fluvialis)。耐药性分析研究表明,19株副溶血性弧菌总体显示对10种常用抗菌药物具有耐药性,其中100%的菌株对四环素、卡那霉素和红霉素表现耐药,94.7%的菌株对氨苄西林耐药、84.1%的菌株对左氧氟沙星和78.9%的菌株对环丙沙星耐药;4株霍乱弧菌和4株河流弧菌对10种抗菌药物的耐药性均较强,但敏感度低,两者对复方新诺明均表现为中介敏感。本研究提示,北海近岸养殖海域中弧菌种类较多,其中以副溶血性弧菌为主,大多对常用抗菌药物具有耐药性,对北海近岸海水养殖疾病防治具有一定的指导意义。  相似文献   

Effect of dietary inclusion of spray‐dried porcine plasma on performance,some physiological and immunological response of broiler chickens challenged with Salmonella sofia          下载免费PDF全文

S. S. M. Beski  R. A. Swick  P. A. Iji 《Journal of animal physiology and animal nutrition》2016,100(5):957-966

This study was conducted to investigate the effect of spray‐dried porcine plasma (SDPP) in broiler chickens under Salmonella sofia disease challenge. The experiment comprised five starter diets: positive control (no supplement), diet supplemented with in‐feed antibiotics (IFA; salinomycin 0.05% + zinc bacitracin 0.033%) and diets supplemented with SDPP at 10 or 20 g/kg diet. All four of these groups were challenged with S. sofia, while a fifth group was unchallenged and used as the negative control. The experimental diets were fed to 14 days; then, the birds were switched to commercial‐type grower and finisher diets. Oral inoculation of the challenged groups with S. sofia occurred on day 8, 10 and 12. Body weight was significantly higher in the birds fed diets containing IFA and SDPP than in the challenged control group, but it was only significant in starter and grower phases. In general, there was an improvement in the weights of the immune‐related organs, but it was only significant for the weight of the bursa of SDPP‐fed birds at 13 days. At day 13, blood potassium content was lower and the concentrations of IgG and IgM tended to be lower in the birds fed on low‐SDPP starter diets than those of the other groups. There were significant differences in the concentration of lactic acid in the ileum and acetic acid, formic acid, butyric acid and propionic acid in the caeca. Inclusion of SDPP to the starter diets of broiler chicks had positive effects on broiler performance, immunity and gut health during exposure to highly pathogenic conditions.  相似文献   

Opportunities and Challenges in Chinese Housing Industry     

FU Hong yuan 《保鲜与加工》2001,(3):67-71

This article studies the opportunities and challenges in housing industry in China and proposes its development tendency.  相似文献