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AIM: The research was to investigate the effects of the Tian ma gou teng decoction on the electric physiology feature of L-type calcium channels in the vascular smooth muscle cells in spontaneously hypertensive rats (SHR), and to further explain the mechanism of the Tian ma gou teng decoction in the intervention of blood pressure.METHODS: 12-week-old SHRs were assigned randomly into five groups：group A (treated with Tian ma gou teng decoction), group B (treated with Tian ma gou teng decoction with subtraction concha haliotidis), group C (treated with nifedipine), group D (treated with concha haliotidis), group E (treated with normal saline as control), each group consisted of 9 rats. After treatments were conducted for 4 weeks, the free calcium concentration in serum was measured. The electric physiology feature of L-type calcium channels in the vascular smooth muscle cells was analyzed by patch clamp technique (PCT).RESULTS: No significant difference between group A and group C was observed in the serum free calcium concentration (P>0.05). There were significant differences among group B, group D and group E (P<0.05), compared to before treatment, the change in group E was the most obvious. A decrease in the L-type calcium channel current of vascular smooth muscle cells was observed in group A and group C. The function of group D was feeble, no decrease in the L-type calcium channels current of vascular smooth muscle cells was observed in group B and group E.CONCLUSION: Tian ma gou teng decoction can increase the serum free calcium concentration and block the L-type calcium channel current in vascular smooth muscle cells, indicating one of the mechanism of intervention of blood pressure. 相似文献
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从河北省兔场分别单卵囊分离孢子化大型艾美耳球虫卵囊、黄艾美耳球虫卵囊及肠艾美耳球虫卵囊,接种无球虫兔后获得纯种卵囊,CTAB法提取孢子化卵囊基因组DNA。利用艾美耳属球虫18SrDNA和5．8SrDNA保守引物,PCR扩增3种兔球虫ITS-1片段,产物纯化后测序。将3种球虫ITS-1测序结果与GenBank发布的兔球虫ITS-1序列进行比对和遗传距离比较,绘制系统发育树。结果表明,大型艾美耳球虫、黄艾美耳球虫及肠艾美耳球虫河北株分别扩增出424、455、434bp的ITS-1片段。大型艾美耳球虫、黄艾美耳球虫及肠艾关耳球虫河北株与GenBank中发布的同种兔球虫ITS-1序列相似性分别为97．4％、97．9％和96．9％。系统发育树显示兔球虫ITS-1序列形成1个单系群,该单系群根据寄生部位分为2个姊妹群。 相似文献
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日粮中添加黄秋葵茎叶粉对大麻花鸡皮肤及脂肪着色的影响 总被引：8，自引：2，他引：6
liuguodao@scuta.edu.cn 《中国农学通报》2006,22(3):11-11
采用单因子试验设计,选取50日龄体重和脚胫颜色相近的大麻花鸡288只,随机分为6个试验处理,每个处理设置3个重复,每个重复16只鸡,试验组为占日粮2%、3%、4%、5%的黄秋葵,对照组为不添加黄秋葵组、添加加丽红和加丽黄的混合组,试验共进行42d。研究结果表明:日粮添加3%、4%、5%黄秋葵试验组显著提高了试验鸡皮肤和脂肪的着色效果(p<0.05),而添加2%黄秋葵试验组和2个对照组的着色效果较差;试验组鸡从开始摄取含黄秋葵的饲料到皮肤显现较黄颜色,需要3周左右,4周后达到比较理想的程度,各个部位着色程度大小是:脚胫>胸部>臀部>脂肪>背部;日粮添加黄秋葵茎叶粉对试验鸡的日增重和料重比无显著影响(p>0.05)。 相似文献
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甜樱桃“红玛瑙”的光合特性研究 总被引：3，自引：0，他引：3
以甜樱桃品种"红玛瑙"为试材,用LI-6400光合作用测定系统对其光合特性进行了研究。结果表明,红玛瑙樱桃净光合速率日变化呈双峰曲线,中午前后光合速率下降,出现"午休现象"。净光合速率年变化呈双峰曲线。红玛瑙樱桃光饱和点为2 362.5μmol.m-2.s-1,光补偿点为31.94μmol.m-2.s-1,CO2饱和点和补偿点分别为1 245μmol CO2.mol-1和65.72μmol CO2.mol-1,光合最适温度为27.75℃。 相似文献
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为了研究北沙参对盐胁迫的响应,利用不同比例盐碱土栽培和不同浓度盐水浇灌处理,检测其生物生长量,以及叶片组织叶绿素a、叶绿素b、总叶绿素、胡萝卜素及丙二醛含量等。结果表明,低比例盐碱土和低浓度盐水对北沙参生长量和叶绿素含量影响不大,高比例盐碱土和高浓度盐水明显降低北沙参的生长量和叶片叶绿素含量,并且盐分胁迫对叶绿素a含量的影响大于对叶绿素b的影响,盐胁迫对胡萝卜素含量的影响不大。当盐碱土比例超过1/2时,北沙参生长受到明显抑制,叶绿素含量显著降低;当盐浓度超过9 g/L时,北沙参生长受到明显抑制,叶绿素含量显著降低。叶片组织丙二醛的含量随盐浓度的增加而升高。 相似文献
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研究了镉毒害对玉米幼苗细胞膜透性和保护酶活性的影响。结果表明,随着镉浓度的增加,ＣＡＴ和ＳＯＤ活性降低,ＰＯＤ活性增强,ＭＤＡ含量上升,质膜透性增大,幼苗的生长受到抑制 相似文献
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动力压辊是用于单板旋切的新技术,它能对木段提供辅助转矩并有防弯作用。故可用于腐软芯材旋切并提高单板出材率及旋切质量。本文介绍了动力压辊装置的结构、工作原理、主要参数的确定及其计算方法。 相似文献