Increased expression of CD40 ligand by peripheral blood mononuclear cells in patients with systemic lupus erythematosus     

LI Yu-jie  LI You-ji  YE Ren-gao  KONG Qing-yu  DONG Xiu-qing  YU Xue-qing 《园艺学报》2003,19(4):514-517

AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE.  相似文献   

小麦矮腥黑粉菌g9890基因编码效应蛋白的生物信息学分析及亚细胞定位     

许停  刘太国  高利  陈万权 《植物保护学报》2023,50(4):866-872

为明确小麦矮腥黑粉菌Tilletia controversa g9890基因编码效应蛋白的生物学功能,根据小麦矮腥黑粉病菌转录组测序结果,筛选出效应蛋白g9890,通过PCR技术获得g9890基因cDNA的全长序列,并对其进行生物信息学以及亚细胞定位分析。多种生物信息学数据库分析表明,g9890基因全长为1 038 bp（包括终止密码子）,共编码345个氨基酸,相对分子质量为37 353.32,理论等电点为5.02。g9890效应蛋白不稳定系数为15.94,疏水性指数为-0.312,是一种亲水性且稳定的蛋白。将g9890基因与pbin-GFP载体重组,利用冻融法转化至根癌农杆菌Agrobacterium tumefaciens GV3101中,将其注入烟草进行瞬时表达分析,并通过共聚焦激光显微镜观测该基因的定位状况,结果显示,g9890定位在细胞膜和细胞核上。  相似文献   

区域气候模式PRECIS对中国气候的长期数值模拟试验   总被引：5，自引：0，他引：5  

王芳栋  许吟隆  李涛 《中国农业气象》2010,31(3):327-332

利用欧洲中心ERA40再分析资料驱动区域气候模式PRECIS,对中国地区进行了50km水平分辨率、40a(1961-2000年)时间长度的连续积分,并对模拟的温度和降水与观测资料进行了比较分析。结果表明:模式对中国地区温度和降水的年、季空间分布型态均具有一定的模拟能力,对温度和降水的年际变化也有较好的模拟效果,尤其对于20世纪80年代后期出现的暖冬以及1998年的夏季极端降水都有较好的体现。但模式不足在于区域内普遍存在模拟的气温略高于实况的系统性暖偏差,幅度一般在1~3℃;内蒙古和东北地区年降水量比观测值明显偏多,长江以南地区和四川盆地的降水中心范围都明显偏大,位置偏南。总体来看,无论是空间分布还是年际变化,模式对温度的模拟能力明显优于降水。  相似文献   

气候变暖对新疆核桃种植气候适宜性的影响   总被引：2，自引：0，他引：2  

普宗朝  张山清 《中国农业气象》2018,39(4):267

基于新疆102个气象台站1961-2015年逐日平均气温、最低气温、最高气温资料,采用线性趋势分析、累积距平、t检验以及ArcGIS的空间插值技术,对影响新疆核桃种植的关键气候因子（≥10℃积温、最低气温≤-25℃日数、最高气温≥40℃日数、终霜冻日早于≥10℃初日天数）的时空变化特征进行分析的基础上,结合核桃种植气候适宜性区划指标,研究了气候变化对新疆核桃种植气候适宜性及其分布的影响。结果表明：新疆≥10℃积温的空间分布总体呈现“南疆多,北疆少;平原和盆地多,山区少”的格局,最低气温≤-25℃日数有“南疆少,北疆多;平原和盆地少,山区多”的特点,夏季最高气温≥40℃日数为“东部多,西部少;平原和盆地多,山区少”的特点;终霜冻日早于≥10℃初日的天数呈现“西部多,东部少;山区多,平原和盆地少”的格局。在上述气候要素空间分异的综合作用下,新疆核桃种植的气候适宜区主要在塔里木盆地西部平原;次适宜区在塔里木盆地大部和吐哈盆地南部;北疆大部,阿尔泰山、天山和昆仑山区以及吐哈盆地、塔里木盆地东部为核桃不适宜种植区。在气候变暖背景下,近55a新疆≥10℃积温、最高气温≥40℃日数和终霜冻日早于≥10℃初日的天数分别以64.7℃·d·10a-1、0.48d·10a-1、0.120d·10a-1的倾向率呈显著（P＜0.05）增多趋势,冬季日最低气温≤-25℃日数以-0.980d·10a-1的倾向率呈极显著（P＜0.001）减少趋势。上述各要素分别于1986年和1997年发生了突变,受其影响,1997年后较其之前,新疆核桃种植的气候适宜区和次适宜区明显扩大,而不适宜区明显减小,气候变暖对新疆核桃种植总体趋于有利。  相似文献   

盐胁迫下栽培大豆黑农40不同器官中抗氧化酶活性的变化   总被引：1，自引：0，他引：1  

付畅  关旸 《大豆科学》2007,26(3):343-346,354

通过对盐胁迫下栽培大豆黑农40的根、茎、叶等不同器官中主要的抗氧化酶活性的测定与分析,探讨了大豆耐盐性的机理.结果表明,黑农40叶片中的SOD、POD和APX活性水平明显高于根部和茎部.叶片中的POD和APX在高盐胁迫下的应答反应都比在低盐时快速.盐胁迫时间的延长引起根、茎、叶中的SOD和POD活性发生明显的下降,在高盐胁迫下这种下降比在低盐时更为急剧.  相似文献   

中国茶园有害生物防控40年     

陈宗懋  蔡晓明  周利  边磊  罗宗秀 《中国茶叶》2020,(1):1-8

总结了改革开放40年来,我国茶园有害生物防控领域取得的重要研究进展,分析了现阶段我国茶园有害生物防控存在的问题,提出了相应的发展对策,为我国茶园有害生物防控研究工作提供参考。  相似文献   

广谱持久抗病基因Pi40在云南高原粳稻的应用     

邹茜  董丽英  袁平荣  刘慰华  苏振喜  金雁  寇姝燕 《农业科学与技术》2017,18(2)

对持有主效广谱抗瘟基因Pi40的单基因系4163与其它11份单基因鉴别品系进行自然条件下稻瘟病抗性鉴定.结果表明,4163在云南省粳稻区的稻瘟病重病区均表现抗病,即在保山、宜良、玉溪叶瘟田间抗性分别为0级、0级、4级,穗瘟田间抗性为3级.同时,利用49份有代表性的云南稻瘟病单胞菌株,通过室内接种抗性鉴定方法建立了Pi40对云南稻瘟病的抗谱,并与另外25份抗稻瘟病单基因系进行室内接种同步鉴定比较.结果表明,Pi40对49份云南地方稻瘟病菌株的抗病频率为87.8％,表现出较广的抗谱,可以作为云南高原粳稻抗病育种的新抗源利用.  相似文献   

表达hTERT及SV40T永生化奶牛乳腺上皮细胞系的建立     

上官陶  王洪梅  冯敏燕  仲跻峰  何洪彬 《山东农业科学》2013,45(2):1-4

构建pcDNA3.1-hTERT、pcDNA3.1-SV40 T载体,线性化后,共转染荷斯坦奶牛乳腺上皮细胞,研究人端粒酶逆转录酶(hTERT)和猿猴病毒40大T抗原(SV40 T)对荷斯坦奶牛乳腺上皮细胞体外培养的作用,并对细胞进行RT-PCR检测分析及免疫荧光鉴定。结果表明,hTERT和SV40 T在奶牛乳腺上皮细胞中表达可以有效地延长细胞的体外培养时间,增加细胞传代次数,获得的细胞系可以正常表达角蛋白。说明在体外培养的乳腺细胞中共表达hTERT和SV40 T可以有效延长细胞寿命且不影响乳腺细胞特性。  相似文献   

Determination of primary structure of amberjack myosin heavy chain and its relationship with structural stability of various fish myosin rods   总被引：1，自引：0，他引：1  

Reina Kawabata  Nobuyuki Kanzawa  Masahiro Ogawa  Takahide Tsuchiya 《Fish physiology and biochemistry》2000,23(4):283-294

The structural stability of fish myosin depends upon species and temperatures of water in which fish live. Primary, secondary, and quaternary structures of myosin heavy chain (MyHC) from three species of fish living at different temperature ranges have been compared with those of rabbit MyHC in order to investigate the differences in stability. Primary structure of MyHC, although being accessible for warm-water and cold-water fish (carp and walleye pollack), was not available in previous for tropical-water fish literature; so in this study primary structure of MyHC of the tropical-water fish amberjack has been determined by cloning and sequencing its cDNA. The MyHC has 1938 amino acid residues (AA), which are almost as much as as those of carp and walleye pollack. The amberjack MyHC is 91–95% homologous with other fish and rabbit MyHCs. There is a discernible difference between animal species with stable myosin rod (amberjack, carp, and rabbit) and walleye pollack with unstable rod. Stable rod species have a high probability of forming coiled-coil around the COOH-terminal end of the rod, while the pollack has a low coiled-coil formation probability. In addition, the average scores of the coiled-coil for myosin rod were rabbit (1.738) > amberjack (1.691) > carp (1.680) > walleye pollack (1.674) which correlated exactly with the observed stability. The results suggest that coiled-coil forming ability, particularly around the COOH-terminal end, directs structural stability of fish myosin rod.  相似文献   

CD40 induces an antimicrobial response against the intracellular pathogen Streptococcus agalactiae in Nile tilapia,Oreochromis niloticus     

Hongli Xia  Huimin Fan  Meng Long  Jun Cheng  Wenjie Chen  Dapeng Yu  Liqun Xia  Yishan Lu 《Journal of fish diseases》2021,44(1):45-52

Streptococcus agalactiae is a Gram‐positive facultative intracellular bacterium that leads to severe economic loss of tilapia worldwide. Previous studies demonstrated that CD40 contributes to host protection against intracellular injection. In this study, CD40 was characterized from Nile tilapia (Oreochromis niloticus), named OnCD40. Sequence analysis showed that open reading frame of OnCD40 was 933 bp, containing a single peptide, a transmembrane domain and four cysteine‐rich domains. The qRT‐PCR revealed that OnCD40 was expressed in all examined tissues with the most abundant ones in spleen and thymus. After S. agalactiae stimulation, the expression of OnCD40 was significantly induced in most of the detected organs. Moreover, OnCD40‐overexpressing fish elicited significant protection against subsequent S. agalactiae challenge; approximately 10000‐fold fewer bacteria were detected in spleen of OnCD40‐overexpressing fish in comparison with control fish. Thus, CD40 had protecting function in Nile tilapia against intracellular pathogens.  相似文献