排序方式： 共有35条查询结果，搜索用时 15 毫秒
1.
比格犬人工感染埃立克体病的临床研究 总被引：2，自引：0，他引：2
为了表述比格犬感染埃立克体（Ehrlichia)后的临床表现，用已确证为Ｅ．platys和Ｅ．canis混合感染的犬血对５条比格犬进行了人工感染，并对临床和血液学变化进行了观察。埃立克体病的潜伏期为６－２９天，其后的临床表现为急性发热、眼结膜苍白、厌食、精神沉郁、体重严重减轻、腹泻带血、多处有淤血斑点出现、６－４５天内全部死亡。血液学变化显示血红蛋白和白细胞明显减少（Ｐ＜０．０１，Ｐ＜０．０５）。由此可见，比格犬对２种埃立克体（Ｅ.canis和Ｅ．platys）混合感染的性很高，严重的临床表现与美国学者普遍认为的亚临床状态相左，这可能是中国Ｇzh981株（Ｅ．platys)和Ｇzh982株（Ｅ．casnis)与国外株在毒力上的差异所致。 相似文献
2.
旨在评价不同剂量伊曲康唑-环糊精制剂通过皮下注射途径对健康比格犬肝肾功能及血常规的影响。选用体重±10 kg的健康纯种比格犬32只,按试验要求随机分为4组,每组8只,建立稳定的生活制度。分别按照0.25 mg/kg、0.75 mg/kg和1.25 mg/kg剂量,皮下注射伊曲康唑制剂,3日1次,连续7次,对照组注射生理盐水。于试验过程中第0、5、10、15、19天采集相关样品,对犬临床表现、血常规指标、血液生化指标、尿常规指标及肝肾功能进行全面的评价。结果表明,给药组与对照组相比,除白细胞相关指标外,其余指标都在正常范围内。给药组在试验过程中均出现白细胞升高现象,但在试验结束后5 d内均恢复正常。因此,伊曲康唑-环糊精制剂作为犬皮下注射剂型,具有良好的安全性和耐受性,操作方便,动物依从性好,适合兽医临床应用。 相似文献
3.
4.
[目的] 研究头孢氨苄片受试制剂和参比制剂在比格犬体内的生物等效性。[方法] 采用双周期和双序列交叉设计,将22只健康比格犬随机分成2组,按30 mg/kg BW分别单剂量口服头孢氨苄片受试制剂Trolevis®300和参比制剂Rilexine®300,于给药前（0 h）和给药后0.5、1、1.5、2、2.5、3、4、6、8、12、17和24 h从臂头静脉采血。对超高效液相色谱串联质谱（UPLC-MS/MS）方法进行特异性、线性、检测限、准确度、精密度、稳定性等方法学考察。利用建立好的UPLC-MS/MS方法测定血浆中的药物浓度,并用WinNonlinTM 8.1软件对药代动力学参数进行分析计算。[结果] 方法学结果显示,在100~5 000 ng/mL浓度范围内相关性良好,相关系数（R2）≥ 0.99,标准曲线方程为y=10.6828x-176.481;高、中、低3个浓度的相对回收率平均值分别为105.63%、104.35%和102.40%;日内和日间变异系数均<15%;检测限为50 ng/mL,定量限为100 ng/mL。药代动力学结果显示,参比制剂组和受试制剂组药代动力学参数如下：Tmax分别为（1.77±0.55）和（2.70±4.68）h;Cmax分别为（28.09±5.09）和（26.82±7.94）μg/mL;T1/2分别为（3.39±1.43）和（3.12±1.05）h;AUC0-t分别为（121.81±25.80）和（116.34±36.30）μg·h/mL。受试制剂与参比制剂药时曲线相似,且受试制剂与参比制剂Cmax、AUC0-t和AUC0-∞几何均数的比值分别为99.51%、99.27%和99.30%,其90% CI均在80.00%~125.00%之间。[结论] 本试验建立的UPLC-MS/MS方法准确、可靠,可用于头孢氨苄的浓度测定,且头孢氨苄受试制剂与参比制剂是等效的,临床上均可用于相关疾病的治疗。 相似文献
5.
Sydney Mukaratirwa Claudio Petterino Alys Bradley 《Journal of toxicologic pathology》2015,28(3):177-180
A single mass was found on the left submandibular salivary gland at necropsy of a 15-month-old male commercially bred laboratory Beagle dog from a control dose group from a repeat toxicity study. Microscopically, the mass was composed of a well-demarcated area of coagulative necrosis surrounded and separated from the normal salivary gland tissue by a thick fibrovascular capsule. Necrosis was admixed with areas of hemorrhage, fibrin, edema, fibrinoid necrosis of the vascular tunica media, and thrombosis of small and large vessels. Within the necrotic tissue, there was marked ductal hyperplasia, and squamous metaplasia of duct and acinar epithelium. The mass was diagnosed as necrotizing sialometaplasia of the submandibular gland. Hyperplastic ductal elements and squamous metaplasia can be mistaken microscopically with squamous cell carcinoma. Therefore, pathologists should be aware of this lesion as to avoid errors in the diagnosis of this benign pathologic condition. 相似文献
6.
D.P. Berry M.C. McClure M.P. Mullen 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2014,131(3):165-172
The objective of this study was to evaluate, using three different genotype density panels, the accuracy of imputation from lower‐ to higher‐density genotypes in dairy and beef cattle. High‐density genotypes consisting of 777 962 single‐nucleotide polymorphisms (SNP) were available on 3122 animals comprised of 269, 196, 710, 234, 719, 730 and 264 Angus, Belgian Blue, Charolais, Hereford, Holstein‐Friesian, Limousin and Simmental bulls, respectively. Three different genotype densities were generated: low density (LD; 6501 autosomal SNPs), medium density (50K; 47 770 autosomal SNPs) and high density (HD; 735 151 autosomal SNPs). Imputation from lower‐ to higher‐density genotype platforms was undertaken within and across breeds exploiting population‐wide linkage disequilibrium. The mean allele concordance rate per breed from LD to HD when undertaken using a single breed or multiple breed reference population varied from 0.956 to 0.974 and from 0.947 to 0.967, respectively. The mean allele concordance rate per breed from 50K to HD when undertaken using a single breed or multiple breed reference population varied from 0.987 to 0.994 and from 0.987 to 0.993, respectively. The accuracy of imputation was generally greater when the reference population was solely comprised of the breed to be imputed compared to when the reference population comprised of multiple breeds, although the impact was less when imputing from 50K to HD compared to imputing from LD. 相似文献
7.
8.
比格犬神经垂体存在内分泌样细胞 总被引：1，自引：0，他引：1
透射电镜观察表明,比格犬(Beagle)神经垂体内除含大量的无髓神经纤维、神经纤维膨体(赫令小体)、神经胶质细胞和血窦外,还可分辨出少量的肥大细胞和内分泌样细胞。内分泌样细胞的主要特征是：胞质内含有大量高电子密度的小分泌颗粒,颗粒呈圆形,表面有膜包裹,直径约109—213nm,主要分布于一侧胞质内。 相似文献
9.
Abstract— Humoral antibody responses to experimental Sarcoptes scabiei var. vulpes infection in 10 beagle dogs were demonstrated by an ELISA in three experiments. In the first experiment, four dogs, infected with a relatively high dose of S. scabiei, seroconverted 2 weeks post infection. All four animals showed clinical signs of sarcoptic mange. In the second experiment, six dogs were infected with a relatively low dose of S. scabiei. Three of the dogs developed clinical signs of sarcoptic mange and only in these three were antibodies to S. scabiei demonstrated. Seroconversion occurred 4–5 weeks post infection. The six dogs infected with a low dose of Sarcoptes were re-infected with a relatively low dose of mites 7 weeks after the spontaneous clinical recovery of the three dogs that had exhibited mange following the primary infection. The later three dogs showed a specific antibody response within 1 week and the other three dogs within 2 weeks following the re-infection. Resumen En tres experimentos se demostraron las respuestas humorales de anticuerpos a la infección con Sarcoptes scabiei var. vulpes en 10 perros de la raza Beagle por medio de pruebas ELISA. En el primer experimento cuatro de los perros infectados con una dosis relativamente alta de Sarcoptes, seroconvirtieron 2 semanas despues de la infección. Estos cuatro animales mostraban cuadro clínico de infección. En un segundo experimento seis de los perros se sometieron a una dosis relativamente baja del ácaro. Solo tres de estos perros demostraron cuadro clínico de la infección, y en éstos animales exclusivamente se encontraron anticuerpos de S. scabiei. Cinco o seis semanas después de la infección se produjo la seroconversión de los mismos. Los seis perros infectados con una dosis baja se volvieron a re-infectar con otra dosis relativamente baja siete semanas después de la recuperatión clínica espontánea de los tres perros que habían padecido el cuadro clínico de la sarna, después de la infección previa. Los últimos tres perros produjeron una respuesta de anticuerpos específica en una semana, y los otros tres perros en dos semanas después de la infección. 相似文献
10.
Objective To evaluate effects of Coherin? on intraocular pressure (IOP), pupil size (PS), and heart rate (HR) in glaucomatous Beagles in single‐dose studies in a pilot study. Materials and methods Intraocular pressure, PS, and HR were measured in eight glaucomatous Beagles. One randomly chosen eye received single 50 μL doses of differing concentrations of Coherin? (treated eye) or vehicle (placebo‐treated eye), and the fellow eye served as the untreated control. After the first measurements, a single dose of either Coherin? or sterile water vehicle was instilled in the drug and placebo eyes, respectively. Results The mean ± SEM diurnal changes in IOP after 0.005%, 0.01%, 0.2%, 0.284%, 1%, 2%, and 4% topical Coherin? once daily were 7.6 ± 3.2 mmHg, 15.5 ± 5.3 mmHg, 11.2 ± 4.4 mmHg, 11.8 ± 4.4 mmHg, 19.1 ± 3.8 mmHg, 5.0 ± 1.8 mmHg, and 8.8 ± 2.8 mmHg, respectively. The declines in IOP were significantly different (P < 0.05) from the untreated control eyes with the 0.2% and 0.284% Coherin?‐treated eyes and suggestive for 1% Coherin? concentrations. No signs of irritation, significant PS, and HR changes were detected in the Coherin?‐treated eyes. Conclusion Of seven different concentrations, 2% and 0.248% Coherin? produced significant declines in IOP in the glaucomatous beagle in single‐dose studies when compared to both untreated control and placebo‐treated eyes. One percent Coherin? solution produced significant IOP decreases compared with the placebo‐treated eye but not the untreated control eyes. No local ocular irritation, PS and HR changes were observed in Coherin?‐treated eyes. This pilot study suggests that topical Coherin? has potential as an ocular hypotensive agent. 相似文献