猪源隐孢子虫感染小鼠模型的建立     

《中国兽医科技》2006,36(4):301-305

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火鸡隐孢子虫在鹌鹑体内发育史的研究   总被引：10，自引：3，他引：7  

查红波  蒋金书 《畜牧兽医学报》1994,25(3):273-278

分离自鹌鹑的火鸡隐孢子虫经口感染２日龄鹌鹑，潜在期为３ｄ，开放期为４－１８ｄ。它在鹌鹑体内发育史过程为：卵巢经口接种后，于十二指肠，空肠，回肠肠腔中子孢子脱囊，至接种后６ｈ全部子孢子脱囊完毕。接种后６ｈ在小肠中出现滋养体。接种后１２ｈ在小肠中出现内含８个裂殖子的裂殖体及裂殖子，接种后４８ｈ滋养体和裂殖体开始出现于直肠。接种后５４ｈ，在小肠，直扬中出现含有１６个子弹形小配子的小配子体。小配子与大配子  相似文献   

以CP23重组蛋白为抗原建立检测微小隐孢子虫抗体间接ELISA方法的研究   总被引：3，自引：0，他引：3  

何宏轩  张西臣  尹继刚  李建华  杨举 《中国兽医杂志》2003,39(1):3-5

摘要：以在E.coli高效表达的微小隐孢子虫子孢子表面抗原CP23为抗原，以辣根过氧化物酶(HRP)标记的山羊抗鼠IgG为二抗，建立了检测微小隐孢子虫抗体的间接ELISA方法。经检测筛选出最佳反应条件为1μg／孔纯化的E.coli表达的CP23抗原包被酶标板，用10％免血清进行封闭，以正常E.coli裂解上清液稀释待检血清。实验表明应用CP23重组蛋白作为诊断C.parvum抗原具有特异性高、抗原易纯化和成本低等特点。  相似文献   

我国羊隐孢子虫感染情况及危险因素     

高海慧  王建东  康晓冬  王培柱  吴学青  李颖康 《中国兽医学报》2021,(2):401-406

隐孢子虫是可以感染小反刍动物的肠道原生生物,具有潜在的公共卫生学问题。隐孢子虫可引起人和动物的腹泻和肠道疾病,严重情况下会出现死亡。被感染的动物可能是人畜共患型隐孢子虫的宿主,可引起公共卫生风险、农场利润减少和动物福利等问题。隐孢子虫病已被报道是新生牛、羊等反刍动物腹泻和死亡的一个重要原因,认为是新生羔羊腹泻的第二大诱因,仅次于轮状病毒。羊隐孢子虫病在世界范围内都有发现,不同程度地威胁着人和动物的身体健康,基于此,现就隐孢子虫对羊感染的危险因素和常用检测方法,以及我国羊感染的现状进行综述,为其防控提供参考。  相似文献   

Molecular and biological characterisation of Cryptosporidium in pigs   总被引：1，自引：0，他引：1  

Morgan UM  Buddle JR  Armson A  Elliot A  Thompson RC 《Australian veterinary journal》1999,77(1):44-47

OBJECTIVE: Genetic and biological characterisation of 12 isolates of Cryptosporidium from pigs and comparing them with Cryptosporidium isolates from humans and cattle. DESIGN: Cryptosporidium isolates from pigs were compared with those obtained from human and cattle using rDNA sequence analysis. The infectivity of two of the porcine isolates was determined in neonatal mice and the clinical history of the infected pigs recorded. RESULTS: Pig-derived isolates of Cryptosporidium exhibited two distinct genotypes; a porcine genotype and a bovine genotype, which is common to cattle and other livestock. The porcine genotype did not produce any infection in neonatal mice whereas the bovine genotype did. CONCLUSION: Two distinct genetically and biologically differing strains of Cryptosporidium appeared to be associated with acute diarrhoea in pigs. Whether Cryptosporidium was a primary or secondary pathogen is unclear but warrants further investigation. As the bovine genotype is known to infect humans, the results suggest that pigs can act as reservoirs of cryptosporidial infections for humans and other live-stock. The zoonotic potential of the pig-adapted genotype is uncertain and requires further study.  相似文献   

In vitro and in vivo efficacy of lasalocid for treatment of experimental cryptosporidiosis   总被引：3，自引：0，他引：3  

Castro Hermida JA  Freire Santos F  Oteiza López AM  Vergara Castiblanco CA  Ares-Mazás ME 《Veterinary parasitology》2000,90(4):265-270

In vitro viability of purified Cryptosporidium parvum oocysts, exposed for 30, 60, 90 and 120 min to 0.27 mg/ml lasalocid suspension was evaluated by inclusion or exclusion of two fluorogenic vital dyes and an excystation technique. Continuously, preventive and curative efficacies at different doses (9, 6.75, 5.625 and 4.5 mg/kg body weight) and regimens of lasalocid against cryptosporidial infection were evaluated on an experimental neonatal mice model. In vitro assays demonstrated a decrease in the oocyst viability related to an increase in exposure time for exposure to the lasalocid suspension. The infection was eradicated when the suspension was administered with a dose of ≥6.75 mg/kg body weight. No apparent toxic effects were observed.  相似文献   

鹌鹑源火鸡隐孢子虫在小白鼠和雏鸡体内内生发育阶段的超微结构比较     

孙铭飞  张龙现  宁长申  菅复春  邵兆霞  梁楠  王进产  王荣军 《畜牧兽医学报》2006,37(7):693-699

用鹌鹑源火鸡隐孢子虫（Cryptos poridium meleagridis）卵囊分别感染昆明系小白鼠和固始雏鸡，用透射电镜和扫描电镜观察比较了C．meleagridis在两种试验动物体内的内生发育虫体超微结构和致病性的差异。利用扫描电镜观察发现，鹌鹑源火鸡隐孢子虫（C.meleagridis）在小白鼠和雏鸡体内的寄生部位有较大差异，在小白鼠体内寄生于十二指肠，但在雏鸡体内主要寄生于回肠；C．meleagridis深嵌于小白鼠肠微绒毛丛内，微绒毛较为完整；但在雏鸡回肠，C．meleagridis似黏附在肠黏膜表面，微绒毛脱落明显，对雏鸡致病性明显比对小白鼠的致病性强。利用透射电镜在两种试验动物的样品中均观察到不同发育阶段的滋养体、裂殖体和大配子体以及正在孢子化的卵囊。滋养体和裂殖体在发育过程中可明显见到粗面内质网结构，裂殖生殖中期阶段粗面内质网尤其发达；小白鼠体内的C．meleagridis虫体与肠黏膜接触处形成一凹陷，寄生部位较深，而在雏鸡体内无此现象。此外，利用透射和扫描电镜均观察到虫体寄生部位周围微绒毛密度高而且也比其它部位长。形成这些差异的原因有待于进一步探讨。  相似文献   

水貂隐孢子虫病流行病学调查及虫种的初步鉴定     

王荣军  张龙现  宁长申  艾伟昌  王海燕  朱惠丽  王永立  卢庆斌  李家诚 《中国兽医寄生虫病》2007,15(1):13-16

为初步了解水貂隐孢子虫病的流行情况,作者于2005年11月份用饱和蔗糖溶液漂浮法检查了河北省肃宁县某水貂养殖场的469份粪便样品。结果,8份粪便样品为隐孢子虫阳性,总感染率为1.71%(8/469)。其中,白貂感染率为2.15%(5/233)、灰貂感染率为2.08%(1/48)、黑貂感染率为1.06%(2/188)。所查的8份隐孢子虫阳性样品均来自5~6月龄的水貂,表明幼龄水貂容易感染隐孢子虫病而老龄水貂不易感染。另外,8份阳性样品多数来自雄性水貂,显示水貂的隐孢子虫感染可能存在性别的差异性。根据卵囊形态和大小将水貂隐孢子虫初步鉴定为小球隐孢子虫(Cryptosporidium parvum)。同时,利用所收集的隐孢子虫卵囊进行了小白鼠感染试验,结果表明水貂源隐孢子虫不感染免疫抑制状态下的昆明系小白鼠。  相似文献   

An outbreak of massive mortality among farm rabbits associated with Cryptosporidium infection     

A. Kaupke  E. Kwit  R.M. Chalmers  M.M. Michalski  A. Rzeżutka 《Research in veterinary science》2014

Cryptosporidium in farm rabbits is not often recognised due to a low prevalence and asymptomatic course of infection. Nonetheless, incidences of fatal diarrhoeic diseases are frequently noticed in the rabbitries. In this article, we report an outbreak where there was massive mortality among farm rabbits associated with Cryptosporidium infection. The disease was characterised by profuse diarrhoea resulting in the death of rabbits. A pooled faecal sample was screened for a presence of parasites using microscopy methods. In the tested sample no other parasites other than Cryptosporidium oocysts were found. Further identification of the parasite species was performed at a molecular level, using the 18 SSU rRNA, COWP and LIB13 PCR followed by a subtyping at the GP60 gene locus. Sequence analysis of GP60 gene fragment revealed the presence of a novel subtype VbA24 of Cryptosporidium cuniculus. In this outbreak a Cryptosporidium protozoan parasite played a major role in the etiology of the gastrointestinal disorders in rabbits resulting in massive mortality of the infected animals.  相似文献   

Infectious agents associated with diarrhoea in neonatal foals in central Kentucky: A comprehensive molecular study     

N. M. Slovis  J. Elam  M. Estrada  C. M. Leutenegger 《Equine veterinary journal》2014,46(3):311-316

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