Systemic Listeria monocytogenes infection and concurrent pleural mesothelioma in a cat     

Jessica A. Elbert  Daniel R. Rissi 《Journal of veterinary diagnostic investigation》2021,33(1):120

Herein we describe a rare case of systemic Listeria monocytogenes infection with concurrent pleural mesothelioma in a stray cat that was found dead and submitted for autopsy. Gross pathology changes consisted of thoracic clear yellow fluid admixed with suspended fibrin strands; clear-to-tan, variably sized, <3 mm diameter pulmonary nodules; and enlargement of the submandibular, retropharyngeal, and prescapular lymph nodes. Histologic changes consisted of extensive areas of suppurative inflammation and necrosis with mineralization that partially effaced the pulmonary parenchyma and lymph nodes. Random, distinct necrotic foci were present throughout the hepatic parenchyma. Extending from the pleura, within perinecrotic alveolar spaces, and infiltrating the submandibular, retropharyngeal, and prescapular lymph nodes were dense sheets of neoplastic epithelioid cells with moderate pleomorphism and occasional karyomegaly and multinucleation. Neoplastic cells exhibited immunolabeling for pancytokeratin AE1/AE3 and vimentin, consistent with pleural mesothelioma. Aerobic bacterial culture of lung yielded heavy growth of L. monocytogenes. Immunohistochemistry (IHC) for L. monocytogenes revealed clusters of bacteria in the lung, lymph node, and liver. Pathologic changes were consistent with systemic listeriosis, confirmed by bacterial culture and IHC, and concurrent pleural mesothelioma.  相似文献   

Development of A Dual Real-time PCR for the Rapid Detection of Listeria monocytogenes     

LI Dan-dan  XU Yi-gang  LI Meng-yuan  WANG Yu  QIU Suo-ping  GAO Hui-jiang  GAO Shen-yang 《中国畜牧兽医》2016,43(6):1453-1457

To establish a rapid assay for Listeria monocytogenes(LM) detection,a Real-time PCR method was developed targeting iap gene of LM.The results showed that the test for 15 bacteria strains,only LM was positive,indicated that the method had high specificity.In addition,the sensitivity of Real-time PCR was 6.5 CFU/mL.Stability and reproducibility of the test showed that the coefficient of variation for the same sample repeat the Ct values were less than 2%.Furthermore,a total of 3 positive samples for LM were detected from 139 clinical samples by the method,which was in accordance with the testing result by GB 478930-2010 standard detection protocol.Therefore,the Real-time PCR method provides a novel rapid,sensitive and good repeatability detection method for LM infection.  相似文献   

产单核细胞李氏杆菌突变株的感染动力学研究     

殷月兰张晨菊  田德斌潘志明 焦新安 《中国兽医科技》2007,37(10):850-853

以BALB/c小鼠为实验动物模型,对actA基因和plcB基因双缺失的产单核细胞李氏杆菌（LM）yzuLM1-2菌株进行感染动力学研究。分别用减毒突变株yzuLM1-2和野生型菌株yzuLM4给BALB/c小鼠静脉注射或灌服,并对脏器中LM的分布动态进行测定。结果显示,在显著高于野生型菌株感染剂量的条件下注射或灌服接种后,减毒突变株组小鼠肝和脾中的LM数量迅速下降,被清除的速度显著快于野生型菌株组。溶血素（LLO）抗体的测定结果显示,减毒突变株菌能和野生型菌同样激发较高水平的抗体。小鼠免疫保护试验结果显示,减毒突变株免疫组对同种血清型LM的攻击具有较好的免疫保护力,可达100%。结果表明,yzuLM1-2突变株侵袭力下降,致病力降低,能激发较高水平的抗体产生,可以作为预防动物李氏杆菌病的候选疫苗菌株,并为用作运送外源保护性抗原的载体提供了材料。  相似文献   

新疆绵羊致病株李斯特杆菌分离与鉴定   总被引：1，自引：0，他引：1  

蒋建军  剡根强  王鹏雁  马勋  王静梅 《中国预防兽医学报》2006,28(6):614-617

本研究采用生化反应，溶血试验，致病力试验，PCR方法对分离的新疆5株绵羊致病株李斯特杆菌90SB1，90SB2，90SB5，90SS1，125SL进行了种型的鉴别测定，并用RAPD技术对这5株李斯特杆菌及标准株李斯特杆菌进行分型。试验结果，五株绵羊致病株（野毒株），在生化反应，培养特性，溶血性，致病性及溶血素基因特异性等方面表现高度的一致性，属于同一种LM。RAPD实验表明此5株LM指纹图谱相同，属于同一个型。此5株致病性LM与标准LM对照株（从食品中分离到）指纹图谱差异显著，属于不同型。  相似文献   

Kinetics of Interferon-Gamma Production and its Comparison with Anti-Listeriolysin O Detection in Experimental Bovine Listeriosis     

Barbuddhe SB  Malik SV  Choudhary SP  Gupta LK 《Veterinary research communications》1998,22(8):505-516

The kinetics of the production of interferon gamma (IFN-) in whole blood culture and its comparison with anti-listeriolysin O (ALLO) detection by ELISA were studied during oral infection of calves with Listeria monocytogenes. Culture filtrate antigen (CFA), listeriolysin O (LLO), and sonicated antigen (SA) were used to prime the peripheral blood mononuclear cells (PBMCs) and the plasma from orally infected calves. IFN- and ALLO appeared as early as day 7 of an oral infection. IFN- was detected earlier with LLO than with SA. The Max50 interleukin (IL-2) activity and IFN- estimated in the culture supernatant from PBMCs primed in vitro with different antigens of L. monocytogenes revealed high induction of IL-2 and IFN- by CFA, LLO and live antigen. IFN- assay and ALLO detection were used for testing cases of repeat breeding in dairy cattle. It appeared that detection of IFN- employing LLO can be used to diagnose listerial infections.  相似文献   

单增李斯特菌inlK基因缺失株的构建及其生物被膜与消毒剂抗力分析     

尉小军  李巧巧  曾东东  蒋建军 《中国畜牧兽医》2022,49(5):1870-1878

【目的】 研究inlK基因对Lm90SB2菌株生物被膜形成能力的影响及其生物被膜与消毒剂抗力的关系,以期为有效防控单增李斯特菌污染提供参考。【方法】 以单增李斯特菌Lm90SB2为试验菌,根据GenBank中公布的单增李斯特菌F2365 inlK基因序列(登录号:AE017262),应用Primer Premier 5.0软件设计用于扩增inlK基因上、下游同源臂片段及验证缺失株的特异性引物,以同源重组技术构建inlK基因缺失株,并通过旁外侧引物运用PCR方法进行缺失株检测。将标准菌株Lm90SB2和构建的缺失株分别培养8、12、24、48 h后进行结晶紫染色,在倒置显微镜下观察形态变化,并用酶标仪测定生物被膜形成能力;用含3 g/L卵磷脂+3 g/L吐温80的PBS溶液和含10 g/L卵磷脂+20 g/L吐温80的PBS溶液作为新洁尔灭消毒剂的中和剂,含5 g/L硫代硫酸钠+5 g/L卵磷脂+20 g/L吐温80的PBS溶液和含10 g/L硫代硫酸钠+30 g/L卵磷脂+20 g/L吐温80的PBS溶液84消毒剂的中和剂,设消毒剂+菌悬液、消毒剂+菌悬液+中和剂、中和剂+菌悬液、消毒剂+中和剂+菌悬液、稀释液+菌悬液(阳性对照)、稀释液+中和剂+培养基(阴性对照)6个试验组,进行中和剂的筛选,并检测不同浓度新洁尔灭(1∶15、1∶30)和84消毒剂(1∶50、1∶100)分别作用1、5、10、20 min时对2株菌的灭菌率。【结果】 PCR结果表明,成功构建了缺失株Lm90SB2ΔinlK,且inlK基因的缺失导致Lm90SB2菌株生物被膜形成能力显著或极显著下降(P<0.05;P<0.01);含3 g/L卵磷脂+3 g/L吐温80的PBS溶液构成的中和剂可有效中和新洁尔灭消毒剂,5 g/L硫代硫酸钠+5 g/L卵磷脂+20 g/L吐温80的PBS溶液可有效中和84消毒剂。不同比例的新洁尔灭(1∶15、1∶30)和84消毒剂(1∶50、1∶100)消毒剂在1、5、10 min对Lm90SB2ΔinlK株的灭菌率均显著或极显著高于Lm90SB2株(P<0.05;P<0.01),且在20 min时灭菌率均为100%。【结论】 inlK基因的缺失导致Lm90SB2菌株生物被膜形成能力下降,且对消毒剂抗力减弱。  相似文献   

Contamination of Poultry Products with Listeria monocytogenes at Poultry Processing Plants     

Yoshimasa SASAKI  Mika HARUNA  Mariko MURAKAMI  Mizuho HAYASHIDA  Nao TAKAHASHI  Tetsuo URUSHIYAMA  Kazuo ITO  Yukiko YAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):129-132

  相似文献   

In vitro Selection of DNAAptamers and Fluorescence-Based Recognition for Rapid Detection Listeria monocytogenes     

LIU Guo-qing  ;LIAN Ying-qi  ;GAO Chao  ;YU Xiao-feng  ;ZHU Ming  ;ZONG Kai  ;CHEN Xue-jiao  ;YAN Yi 《中国农业科学(英文版)》2014,(5):1121-1129

Aptamers are specific nucleic acid sequences that can bind to a wide range of nucleic acid and non-nucleic acid targets with high affinity and specificity. Nucleic acid aptamers are selected in vitro from single stranded DNA or RNA ligands containing random sequences of up to a few hundred nucleotides. Systematic evolution of ligands by exponential enrichment （SELEX） was used to select and PCR amplify DNA sequences （aptamers） capable of binding to and detecting Listeria monocytogenes, one of the major food-borne pathogens. A simplified affinity separation approach was employed, in which L. monocytogenes in exponential （log） phase of growth was used as the separation target. A fluorescently-labeled aptamer assay scheme was devised for detecting L. monoeytogenes. This report described a novel approach to the detection of L. monocytogenes using DNA aptamers. Aptamers were developed by nine rounds of SELEX. A high affinity aptamer was successfully selected from the initial random DNA pool, and its secondary structure was also investigated. One of aptamers named e01 with the highest affinity was further tested in aptamer-peroxidase and aptamer-fluorescence staining protocols. This study has proved the principle that the whole-cell SELEX could be a promising technique to design aptamer-based molecular probes for dectection of pathogenic microorganisms without tedious isolation and purification of complex markers or targets.  相似文献   

伊犁地区绵羊李氏杆菌药敏特性及平板凝集快速诊断方法的建立     

皮志媛  王一明  张海兰  雷程红 《安徽农业科学》2014,(2):480-482

[目的]建立一种快速检测李氏杆菌病的方法。[方法]通过对实验室保存的5株李氏杆菌分离株进行药敏试验,用甲醛灭活苗免疫试验动物,制备特异性抗体,将特异性抗体与抗原进行平板凝集试验。[结果]分离菌株对氨苄西林、庆大霉素、链霉素高度敏感,对头孢唑啉、左氧氟沙星和多粘菌素中度敏感,对头孢他啶、头孢呋辛钠耐药性较强。通过用甲醛灭活苗免疫试验动物来制备特异性抗体,将特异性抗体与抗原做平板凝集试验,建立一种快速准确检测李氏杆菌病的方法。[结论]成功建立了一种快速检测李氏杆菌病的方法。  相似文献   

单增李斯特菌溶源性噬菌体的诱导及鉴定   总被引：2，自引：2，他引：0  

安慧婷  刘瑾  王娟  姚火春  王恒安 《上海交通大学学报(农业科学版)》2014,32(5):39-43

利用丝裂霉素C诱导获得单增李斯特菌溶源性噬菌体,并确定其核酸类型。PCR及其扩增产物的序列测定检测原噬菌体,透射电子显微镜观察丝裂霉素C诱导获得的溶源性噬菌体颗粒,DNase I、RNase A和绿豆核酸酶分别消化噬菌体基因组,确定噬菌体的核酸类型。结果显示,38株单增李斯特菌分离株中的16株在tRNAArg位点携有原噬菌体,其中1株可诱导出噬菌体颗粒,该噬菌体为长尾噬菌体,核酸为dsDNA。噬菌体的成功诱导为噬菌体及其功能基因的开发利用奠定了基础。  相似文献