Likelihood of pregnancy after embryo transfer is reduced in recipient mares with a short preceding oestrus          下载免费PDF全文

J. Cuervo‐Arango  A. N. Claes  M. Ruijter‐Villani  T. A. Stout 《Equine veterinary journal》2018,50(3):386-390

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Assessment of Uterine Vascular Perfusion During the Estrous Cycle of Mares in Connection to Circulating Leptin and Nitric Oxide Concentrations     

《Journal of Equine Veterinary Science》2016

The objective of this study was to find difference in vascular perfusion of uterine horns or uterine body throughout the estrous cycle and their relation to circulating nitric oxide and leptin concentrations. Five cyclic mares were subjected to transrectal Doppler ultrasonography and blood sampling for 18 days. Area of color and power Doppler modes was measured in pixels. Day (P = .0001) of the estrous cycle and ovulation (P = .0001) influenced uterine blood flow. Uterine body blood flow directed away from the transducer (blue, P = .0001) increased from day −5 until day 0 (day of ovulation), and its power (P = .0001) blood flow increased from day −6 until day 0; then, both decreased until days 12 and 10, respectively. Conversely to the contralateral uterine horn, ipsilateral uterine horn blood flow directed away from the transducer (blue, P = .0001) increased from day −5 until day −1, and its power (P = .0001) blood flow increased from day −6 until day 0; then, both decreased until day 10. Nitric oxide concentrations (P = .0001) attained two major peaks; the first on day −3 and the other persisted from day 2 until day 5. Leptin concentrations increased (P > .001) with a maximum value on day 0 and then decreased until a minimum value on day 9. In conclusion, during the estrous cycle, ipsilateral uterine horn and uterine body blood vessels had similar blood flow. Both leptin and nitric oxide played a role during follicle growth, ovulation, and corpus luteum development and modulated uterine blood flow before and after ovulation.  相似文献   

分娩应激对围产期奶牛免疫机能的影响     

王亮  张克春 《中国奶牛》2012,(19):30-33

本文分析了围产期奶牛的分娩应激对其免疫机能的影响,并从宏观和微观上进行了详细的阐述,为围产期奶牛的饲养管理提供了理论依据。  相似文献   

Molecular changes in the equine follicle in relation to variations in antral follicle count and anti‐Müllerian hormone concentrations          下载免费PDF全文

A. Claes  B. A. Ball  M. H. T. Troedsson  T. E. Curry Jr.  E. L. Squires  K. E. Scoggin 《Equine veterinary journal》2016,48(6):741-748

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产前不同营养水平对围产期健康奶牛血清总胆红素、蛋白及转氨酶的影响   总被引：1，自引：1，他引：1  

左之才  邓俊良  王哲  张乃生  李艳飞  牛淑玲  李红梅  刘彩霞 《中国奶牛》2007,(3):5-9

将健康的围产期奶牛30头随机分为三组，分别于产前28d开始饲喂NRC标准日粮（营养水平100％组，即Ⅰ组）、NRC标准增加20％日粮（营养水平120％组，即Ⅱ组）和NRC标准减少20％日粮（营养水平80％组，即Ⅲ组），产后各组奶牛均饲喂标准泌乳日粮，至产后56d结束，观察干奶期不同营养水平对围产期健康奶牛血清总胆红素、蛋白及转氨酶的影响。试验结果表明，围产期是机体生理状况处于剧烈变化的时期，奶牛产后的蛋白质代谢活动、转氨酶活性较产前明显增强；但健康奶牛均能通过调整糖代谢、脂代谢和蛋白质代谢，促进能量与营养物质间的相互转化，适应机体的各种生理变化。  相似文献   

Immunology of infective preterm delivery in the mare     

S. K. Lyle 《Equine veterinary journal》2014,46(6):661-668

Placentitis is reported to be the cause of 9.8–33.5% of abortions, stillbirths and perinatal losses in horses. Bacterial infections are responsible for 53% of placentitis cases with Streptococcus equi ssp. zooepidemicus being isolated in 28% of these cases. Clinically, mares may have a vaginal discharge, show udder development, lactate prenatally and deliver a premature or dead foal. Major aspects of the pathogenesis of infectious preterm delivery that may require more effective therapeutic targeting are myometrial contraction, immunological aspects of preterm delivery, and the effects of proinflammatory cytokine signalling on activation of the fetal hypothalamic–pituitary–adrenal axis. This article focuses on current knowledge of inflammatory signalling secondary to equine placentitis, and the interplay among inflammation, loss of myometrial quiescence and activation of the fetal hypothalamic–pituitary–adrenal axis.  相似文献   

猪围产期外周血ANAE+T细胞动态观察   总被引：1，自引：0，他引：1  

梁宏德  程相朝  宁长申 《畜牧兽医学报》2002,33(5):517-519

有用酸性α－醋酸萘酯酶（ANAE）染色方法测定了11头猪围产期外周血不同时期总ANAE^ T与大颗粒型ANAE^ T细胞（tANAE^ T细胞与lgANAE^ T细胞）百分率，结果表明，猪分娩前21d至分娩前6d，外周血tANAE^ T细胞与lgANAE^ T细胞百分率均低于分娩后第25d的相应指标，说明猪妊娠后期细胞免疫功能低下；分娩前10d，猪外周血tANAE^ T细胞与lgANAE^ T细胞百分率均较分娩前第21d的相应指标显著升高（P＜0．05），提示分娩的启动可能与母体细胞免疫功能增强，导致免疫排斥的发生有一定关系，分娩后tANAE^ T细胞与lgANAE^ T细胞百分率降低后逐渐升高，tANAE^ T细胞分娩后第10d,lgANAE^ T细胞于分娩后第5d已分别明显高于猪分娩前第21d的相应指标（P＜0．05），猪分娩后第25d的tANAE^ T细胞与lgANAE^ T细胞和正常未妊娠母猪的相应指标接近，表明猪分娩后5－25d细胞免疫功能已渐恢复正常。  相似文献   

沙棘果渣成分分析   总被引：5，自引：0，他引：5       下载免费PDF全文

马养民  姜少娟  孔东宁  史清华  傅建熙  侯慧波  孔阳 《西北农业学报》2005,14(5):125-127

对沙棘果渣的成分进行系统预试和分析测定表明,沙棘果渣含有丰富的有效物质--黄酮,vB1,vB2,vC,vE,氨基酸,粗蛋白,粗脂肪,粗纤维,鞣质,糖,三萜或甾体类等成分,并对主要有效成分作了含量测定,其中黄酮含量高达0.694%;vE含量较高达112.85 mg/100 g;油含量为28.5%,包含了大量对人体有益的不饱和脂肪酸;氨基酸含量丰富、种类齐全,人体必需氨基酸的量约占氨基酸总量的61.75%.沙棘果渣具有潜在的综合利用价值.  相似文献   

孕马血清促性腺激素单克隆抗体的研制   总被引：2，自引：0，他引：2  

马友福  马春艳 《畜牧兽医学报》1996,27(2):125-130

用高纯度孕马血清促性腺激素免疫ＢＡＬＢ／ｃ小鼠，加完全佐剂进行基础免疫，第７日脾脏免疫，第１０日取血测滴度，为阳性则取出脾脏，将脾细胞与Ｓｐ２／０细胞融合，培养并用琼脂单扩法筛选抗体生成孔，ＥＬＩＳＡ复测，得孕马血清促性腺激素抗体阳性杂交瘤细胞株Ｄ６Ｃ１０。经降植烷予处理的ＢＡＬＢ／ｃ鼠腹腔注射Ｄ６Ｃ１０，腹水单抗滴度１０＾６。瘤细胞ＤＮＡ含量测定证实为脾和骨髓瘤细胞的杂交瘤细胞。单抗亚型分析为Ｉ  相似文献   

反亲和层析法纯化PMSG的试验研究     

邓瑞春  崔青山  李振甲  甄英凯 《中国兽医学报》1989,(3)

按PMSG粗品提取方法,从非孕马血清中获得“杂蛋白”提取物。以此“杂蛋白”为抗原制备兔抗“杂蛋白”抗血清,并从中提取IgG,制备多元性抗体免疫吸附柱,然后将PMSG粗品通过此柱,免疫吸附除去其中的杂蛋白,以提高PMSG的纯度和比活性。试验结果,杂蛋白除去率为61.4～70％,PMSG活性回收率为74～89％;PMSG比活性由原来的267IU/mg提高到630IU/mg,纯度提高约2.3倍;通过反亲和层析法部分纯化的PMSG,经电泳分析,主要为α—G部分,兼有少量其他血清杂蛋白。  相似文献