排序方式： 共有9条查询结果，搜索用时 296 毫秒
1
1.
建立了同时测定鸡组织中残留的磺胺对甲氧嘧啶(SMD)和二甲氧苄啶(DVD)了的高效液相色谱-串联质谱法(HPLC-MS/MS)。样品经乙腈提取,取部分提取液用正己烷脱脂后于氮气吹干,残渣用流动相溶解并定容后上机检测,外标法定量。采用C18色谱柱,流动相为乙腈和1mL/L甲酸水溶液。选择1个母离子和2个子离子进行反应监测,对磺胺对甲氧嘧啶和二甲氧苄啶残留进行定性,选择信号最强的子离子进行定量。结果显示,SMD和DVD分别在1ng/mL~200ng/mL和0.5ng/mL~100ng/mL范围内呈良好线性关系,相关系数R20.992。通过空白样品添加回收试验,所有基质中SMD和DVD的定量限分别为10μg/kg和5μg/kg,SMD和DVD分别在50、100、200μg/kg和25、50、100μg/kg 3个添加水平下的回收率为71.7%~115.0%,相对标准偏差为1.8%~13.2%,该方法定量限低,灵敏度高,重现性好,符合相关研究的要求。 相似文献
2.
通过动物体内试验,比较复方磺胺氯吡嗪钠的不同组方与剂型对鸡球虫病的疗效。第一次试验设磺胺氯吡嗪钠+三甲氧苄啶(TMP)溶液组(0.02%,5 d)、磺胺氯吡嗪钠+TMP混悬液组(0.02%,5 d)、磺胺氯吡嗪钠+二甲氧苄啶(DVD)混悬液组(0.02%,5 d)、药物对照组(磺胺氯吡嗪钠可溶性粉,0.03%,5 d)、感染不用药组和不感染不用药组。第二次试验设磺胺氯吡嗪钠+TMP混悬液组(0.02%,5 d)、磺胺氯吡嗪钠+DVD混悬液组-1(0.02%,5 d)、磺胺氯吡嗪钠+DVD混悬液组-2(0.02%,3 d)、药物对照组(磺胺氯吡嗪钠可溶性粉,0.03%,3d)、感染不用药组和不感染不用药组。每组30只鸡。以血便记分、存活率、增重及相对增重率、病变记分与病变值、卵囊产量与卵囊值、抗球虫指数(ACI)等指标综合评定抗球虫效果。结果显示:磺胺氯吡嗪钠+TMP混悬液组(0.02%,5 d)和磺胺氯吡嗪钠+DVD混悬液组(0.02%,5 d)的抗球虫指数始终大于或接近180,为抗球虫高效水平;磺胺氯吡嗪钠+TMP溶液组(0.02%,5 d)、磺胺氯吡嗪钠+DVD混悬液组(0.02%,3 d)的抗球虫指数大于160,为抗球虫中效水平。提示:磺胺氯吡嗪钠+TMP混悬液(0.02%,5 d)和磺胺氯吡嗪钠+DVD混悬液(0.02%,5 d)对鸡球虫有较好的疗效。 相似文献
3.
【目的】建立在猪组织中二甲氧苄啶浓度的检测方法,并对二甲氧苄啶在猪体内的残留消除进行研究,制订该药在猪体内的休药期.【方法】常规饲养条件下,将30头健康猪随机分为6组,按体质量以5 mg·kg-1剂量混饲给药,每天2次,连用7 d,于最后一次给药后6、24、96、168、240和336 h各宰杀1组猪,分别采集每头猪的肌肉、肝脏、肾脏和脂肪组织,采用HPLC法对二甲氧苄啶在各组织的残留量进行测定.【结果和结论】二甲氧苄啶在猪组织中消除较快,给药后第240小时所有检测组织中的二甲氧苄啶已全部低于方法的定量限(40μg·kg-1),二甲氧苄啶在肌肉中消除最快,在肾脏中消除最慢.根据日本肯定列表规定的二甲氧苄啶在禽组织的最大残留限量(50μg·kg-1)和WT1.4休药期软件计算结果,建议该药在猪体内的休药期为8 d. 相似文献
4.
试验旨在建立二甲氧苄啶在猪粪便和尿液中的液相色谱串联质谱检测方法。尿液经乙酸乙酯液液萃取,有机层经氮气吹干、35%乙腈水复溶后上机检测;粪便样品经酸乙酯提取,MCX 小柱净化后,洗脱液用氮气吹干,35%乙腈水复溶后上机检测。结果显示：猪粪便和尿液中二甲氧苄啶浓度分别在5~1 000 μg/kg 和10~1 000 μg/L范围内呈现良好的线性关系渊r>0.99冤,尿液尧粪便中二甲氧苄啶的定量限分别为10 μg/L 和5 μg/kg,尿液添加浓度为10、100、1 000 μg/L时的回收率分别为79.6%、84.3%、90.2%,粪便添加浓度为10、100、1 000 μg/kg 时的回收率分别为66.1%、65.1%、54.7%,批间、批内变异系数均小于14%,该方法定量限低,灵敏、准确,符合相关研究的要求。 相似文献
5.
抗菌增效剂可以增强抗生素的抗菌活性、减少细菌耐药性的产生、提高治疗效果。药动学研究是临床上制定给药方案,评价药效的重要指标;残留消除研究可以了解药物的残留靶组织、残留标识物,进而制定适宜的休药期。作者简述了艾地普林、甲氧苄啶、二甲氧苄啶3种抗菌增效剂的药动学及残留消除规律,着重对比艾地普林与甲氧苄啶在不同动物体内的药动学参数,显示与甲氧苄啶相比艾地普林具有更长的消除半衰期及更大的表观分布容积,且甲氧苄啶在不同动物体内的消除半衰期差异较大,二甲氧苄啶常作为肠道的抗菌增效剂使用。目前多采用高效液相色谱法及和其他色谱联用的方法,对抗菌增效剂进行药动及残留消除研究,并且也证明了该方法专一性好、灵敏度高。当前由于抗生素的不合理使用甚至滥用导致的药物失效以及细菌耐药性的问题越来越普遍,而抗菌增效剂的出现为解决该类问题提供了新的方向。通过对3种抗菌增效剂在不同动物体内的药动学及残留消除研究,有利于深入了解各种药物在不同动物体内共性和差异性规律,以期发现该类药物在临床使用中存在的问题并且不断地完善抗菌增效剂在临床上的应用。 相似文献
6.
Antibacterial synergist can enhance the antibacterial activity of the antibiotics, reduce bacterial resistance and improve treatment effect. Pharmacokinetic study is to develop dosage regimen and evaluate efficacy in clinical.Elimination of residual study can understand drug residual target tissue and residual marker to formulate appropriate withdraw time. This review briefly describes the aditoprim, trimethoprim,diaveridine of three kinds of antibacterial synergist pharmacokinetic and residue elimination rule, emphatically when contrast aditoprim to trimethoprim in the pharmacokinetic parameters of different animals. We can conclude aditoprim with longer elimination half-life and larger apparent volume of distribution, and trimethoprim elimination half-life is different in different animals, diaveridine is often used as intestinal antibacterial synergist. Recently researchers always use high performance liquid chromatography (HPLC) method or chromatographic combination method to study antibacterial synergist on pharmacokinetics and residues elimination research, it shows that the method has good specificity and high sensitivity. Currently due to the irrational use even abuse of antibiotics leading to drug failure and bacterial drug resistance problems, the presence of the antibacterial synergist for solving this problem provide a new direction, and three kinds of antibacterial synergist in different animals pharmacokinetic and residues elimination research is helpful to understand various kinds of drugs commonalities and differences in different animals, in order to find problems of this kind of drug in clinical using and continuously perfect applications of antibacterial synergist in clinical. 相似文献
7.
Yongjun LI Shijin BU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1555-1563
Diaveridine (DVD) is used in combination with sulphachloropyrazine (SPZ) as an effective
antibacterial agent and antiprotozoal agent, respectively, in humans and animals. To gain
a better understanding of the metabolism of SPZ and DVD in the food-producing animals, a
high performance liquid chromatography (HPLC) method to determine and quantify
sulphachloropyrazine (SPZ) and diaveridine (DVD) suspension residues from broilers is
reported. Thirty healthy chickens were orally administered with
sulphachloropyrazine-diaveridine (SPZ-DVD) suspension in water of 300
mg/l (SPZ) per day for seven successive days. Six chickens per day were
slaughtered at 0, 1, 3, 5 and 7 days after the last administration. This procedure
permitted SPZ and DVD to be separated from muscle tissue, liver, kidneys and skin with fat
after extraction with acetonitrile and acetone under slightly acidic conditions. From the
detected residuals in different tissues, we found that SPZ was quickly eliminated in liver
and muscle, and slowly eliminated in kidney and skin with fat. DVD was quickly eliminated
in liver and slowly eliminated in kidney. The withdrawal period of SPZ was 3.26, 3.72,
4.39 and 5.43 days in muscle, liver, kidney and skin with fat, respectively. The
withdrawal period of DVD was 4.77, 4.94, 6.74 and 4.58 days in muscle, liver, kidney and
skin with fat, respectively. Therefore, the suggested withdrawal period for SPZ-DVD
suspension should be 7 days after dosing for seven successive days. 相似文献
8.
TMP和DVD对两种磺胺药抗球虫增效作用试验 总被引：2，自引：0，他引：2
以雏鸡柔嫩艾美耳球虫病为模型,以相对存活率、相对增重率、盲肠病变值和盲肠卵囊值为观察指标,以抗球虫指数(ACI)为判定标准,观察甲氧苄啶(TMP)和二甲氧苄啶(DVD)与不同浓度磺胺喹口恶啉(SQ)和磺胺氯吡嗪钠(Esb3)联合用药治疗效果和增效作用.结果显示,药物对照组0.02%SQ和0.06% Esb3的ACI值分别为151和162;TMP与0.01%、0.02%、0.05%的SQ联用的ACI分别为177、181和195; DVD分别与0.01%、0.02%、0.05%的SQ联用的ACI分别为177、194和194;TMP与0.03%、0.06%、0.12%的Esb3联用的ACI分别为181、188和192;DVD与0.03%、0.06%、0.12%的Esb3联用的ACI分别为185、187和191.结果表明,TMP和DVD能明显提高SQ和Esb3的抗球虫效果, TMP和DVD之间的抗球虫增效作用无明显的差异. 相似文献
9.
选用10日龄健康雏鸡190只,随机分为19组,每组10只。试验组和阳性对照组雏鸡人工感染柔嫩艾美耳球虫,建立雏鸡球虫病模型。在感染后3d开始用药,以存活率、相对增重率、盲肠病变值和盲肠卵囊值为观察指标,以抗球虫指数(ACI)为判定标准,研究二甲氧苄啶(DVD)以1∶5比例与几种磺胺药联合应用的抗球虫增效作用。结果表明,0.06%磺胺二甲嘧啶(SM2)、0.05%磺胺喹恶啉(SQ)、0.05%磺胺-6-甲氧嘧啶(SMM)、0.05%SQ+0.06%磺胺氯吡嗪(Esb3)的ACI分别为161、142、157、147。DVD与不同浓度SM2联用的ACI分别为179、166、155;与不同浓度SQ联用的ACI分别为178、168、152;与不同浓度SMM联用的ACI分别为184、174、163;与不同浓度SQ和Esb3混合组联用的抗球虫指数分别167、176、186。表明DVD与几种磺胺药联用后均产生了抗球虫增效作用。 相似文献
1