马传贫驴白细胞弱毒疫苗株跨膜蛋白主要免疫决定区基因的克隆与表达   总被引：3，自引：0，他引：3  

薛飞  贾斌  朱远茂  杨建德  王晓钧  相文华  赵立平  吕晓玲  沈荣显 《中国预防兽医学报》2003,25(5):321-325

从感染驴白细胞的马传贫驴白细胞弱毒疫苗株前病毒DNA中克隆了编码跨膜蛋白主要免疫决定区(TMIR)的基因，并在大肠杆菌中进行了表达。所表达的融合蛋白有一部分是可溶的，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。在间接酶联免疫吸附试验(ELISA)和免疫印迹试验中，重组的TMIR蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。这表明该重组蛋白具有良好的抗原性和特异性，可用于马传贫弱毒疫苗株在体内外复制、接种马体内免疫应答及马传贫诊断的研究。  相似文献   

5·15绥化局部强风致房屋破坏调查分析   总被引：1，自引：0，他引：1  

白雪 《黑龙江八一农垦大学学报》2012,24(5):17-20

结合现场调查结果,介绍并分析了绥化局部强风区附近建筑物受风灾影响现象。得到以下主要结论:一些按照国家现行规范设计的建筑物可以抵抗超限的水平风荷载和屋面吸力;但是,未经设计自建的农村民宅,在大风作用下其结构或围护结构就会产生严重的破坏。砖木结构的破坏主要表现为围护结构整体受损和山墙倒塌。屋面的抗风能力与屋面材料有关。传统的土坯房及一面清房屋在强风作用下整体倒塌。强风导致供电线路、通讯线路的毁损带来重大经济损失。  相似文献   

马传染性贫血病毒驴白细胞弱毒疫苗株囊膜基因的优化及其DNA疫苗体外瞬时表达研究     

侯绍华  彭金美  周涛  袁秀芳  汪明  童光志 《中国预防兽医学报》2007,29(11):840-847

马传染性贫血病毒(Equine infectious anemia virus,EIAV)的密码子使用频率与哺乳动物间存在着明显差异。为此,对马传染性贫血病毒驴白细胞弱毒疫苗株(DLA-EIAV)囊膜全长基因按照哺乳动物优势密码子的使用原则进行了重新设计和合成,并以此为基础通过重叠延伸PCR、限制酶切等方法得到结合型和分泌型囊膜基因,将其插入含有鸡beta-actin/兔beta-globin复合启动子(AG)的高效表达载体pCAGGS中,构建了EIAV驴白细胞弱毒疫苗株结合型和分泌型囊膜基因的DNA疫苗质粒pCAGGS-opti-bou-env、pCAGGS- opti-sec-env。将构建的质粒纯化后分别转染293T细胞,以间接免疫荧光和Western blot方法检测转染48 h后细胞及上清中囊膜蛋白的表达。结果显示,两种表达质粒均可正确表达EIAV囊膜蛋白,与相对应未优化的表达载体pCAGGS-wt-bou-env和pCAGGS-wt-sec—env相比,密码子优化的基因体外瞬时表达水平有极为显著的提高,而且蛋白表达部位也与预期的结果符合。这一结果为EIAV囊膜蛋白的单抗制备、表位鉴定、免疫试验、新疫苗的开发等奠定了基础。  相似文献   

建筑围护结构动态热特性辨识及其系统     

陈友明  张国强  陈在康 《湖南农业大学学报(自然科学版)》1999,26(4)

提出了建筑围护结构动态热特性辨识的概念，阐述了围护结构动态热特性辨识的方法及其作用，介绍了一种新的围护结构动态热特性辨识系统的构成和特点。  相似文献   

绵羊肺腺瘤病毒致瘤机制研究进展     

刘畅  敖威华  李磊  于立新  么宏强  周建华  马学恩 《动物医学进展》2015,(1):79-82

绵羊肺腺瘤(OPA)是由绵羊肺腺瘤病毒(OPAV)引起的一种慢性、传染性绵羊肺脏肿瘤性疾病,该病对养羊业,特别是种羊的发展有重大影响,世界动物卫生组织将其列为必须通报的动物疫病。论文就近年来国内外关于OPAV致瘤机制的相关研究进行了综述,包括OPAV的转录特异性和囊膜蛋白的致瘤作用和OPAV受体等。这对深入研究OPAV的致瘤机制及确定相应的防控靶点,具有重要的理论意义和潜在的应用价值。  相似文献   

高级别动物生物安全实验室气密性的探讨     

严春炎  李正武 《中国兽药杂志》2014,48(9):57-60

高级别动物生物安全实验室气密性是保证实验室空气洁净度、室内实验人员和室外环境安全的重要手段。我国现行规范对高级别动物生物实验室各区域气密性有严格的规定。从实验室装修、围护结构设备安装以及气密性检测方面对实验室气密性进行了讨论,以期为我国高级别动物生物安全实验室的建设提供参考。  相似文献   

Development and characterization of monoclonal antibodies to subgroup A avian leukosis virus     

Y. Qiu  X. Li  L. Fu  Z. Cui  W. Li  Z. Wu  S. Sun 《Veterinary and comparative oncology》2014,12(1):47-51

Avian leukosis virus subgroup A (ALV‐A) is a retrovirus which infects egg‐type chickens and is the main pathogen of lymphoid leukosis (LL) and myeloid leukosis (ML). In order to greatly enhance the diagnosis and treatment of clinical avian leukemia, two monoclonal antibodies (MAbs) to ALV‐A were developed by fusion between SP2/0 and spleen cells from mice immunized with expressed ALV‐A env‐gp85 protein. Using immunofluorescence assay (IFA), two MAbs reacted with ALV‐A, but not with subgroups B and J of ALV. Western blot tests showed that molecular weight of ALV‐A envelope glycoprotein recognized by MAbs was about 53 kD. Isotyping test revealed that two MAbs (A5C1 and A4C8) were IgG1 isotypes. These MAbs can be used for diagnosis and epidemiology of ALV‐A.  相似文献   

Assessing the potential impacts of climate change and their conservation implications in Japan: A case study of conifers     

Yuko Ogawa-Onishi  Pam M. Berry 《Biological conservation》2010,143(7):1728-1736

The Japanese archipelago is a biodiversity hotspot with a unique regional climate influenced by the Asian monsoon circulation, surrounding seas, and complex topography. Japan has numerous mountains and islands, which are potentially vulnerable to climate change. This study evaluated the potential impact of climate change on species diversity in Japan, using 25 conifer tree species as a case study. We applied ensemble models based on generalised additive models, artificial neural networks, generalised boostedmodels, and random forests to species’ locality records at 1 km² resolution. The results indicated a substantial impact, such that 80% of the species may lose over half of their current climatically suitable areas by 2100. The lower altitudinal range limits were projected to shift upwards by 293 m on average, suggesting that alpine/sub-alpine and sub-boreal species may face widespread local extinctions. The impacts on sub-alpine species may be moderated by assisted migration to the northern island where they currently do not occur. However, climatically suitable areas for these species and sub-boreal species that occur on the island overlapped significantly, indicating that assisted migration may entail the risk of introducing biotic competition or interbreeding. Thus, rugged topography and dispersal barriers between islands are likely to amplify the future impacts of climate change in Japan. Limited areas in the central mountain region were identified as potential bioclimatic refugia, which should be conserved as a priority.  相似文献   

Is the decline of soil microbial biomass in late winter coupled to changes in the physical state of cold soils?     

Robert L. Jefferies  Kate A. Edwards  Jack Dainty 《Soil biology & biochemistry》2010,42(2):129-135

During winter when the active layer of Arctic and alpine soils is below 0 °C, soil microbes are alive but metabolizing slowly, presumably in contact with unfrozen water. This unfrozen water is at the same negative chemical potential as the ice. While both the hydrostatic and the osmotic components of the chemical potential will contribute to this negative value, we argue that the osmotic component (osmotic potential) is the significant contributor. Hence, the soil microorganisms need to be at least halotolerant and psychrotolerant to survive in seasonally frozen soils. The low osmotic potential of unfrozen soil water will lead to the withdrawal of cell water, unless balanced by accumulation of compatible solutes. Many microbes appear to survive this dehydration, since microbial biomass in some situations is high, and rising, in winter. In late winter however, before the soil temperature rises above zero, there can be a considerable decline in soil microbial biomass due to the loss of compatible solutes from viable cells or to cell rupture. This decline may be caused by changes in the physical state of the system, specifically by sudden fluxes of melt water down channels in frozen soil, rapidly raising the chemical potential. The dehydrated cells may be unable to accommodate a rapid rise in osmotic potential so that cell membranes rupture and cells lyse. The exhaustion of soluble substrates released from senescing plant and microbial tissues in autumn and winter may also limit microbial growth, while in addition the rising temperatures may terminate a winter bloom of psychrophiles.Climate change is predicted to cause a decline in plant production in these northern soils, due to summer drought and to an increase in freeze-thaw cycles. Both of these may be expected to reduce soil microbial biomass in late winter. After lysis of microbial cells this biomass provides nutrients for plant growth in early spring. These feedbacks, in turn, could affect herbivory and production at higher trophic levels.  相似文献   

基于Translog生产函数的北京市西瓜最优种植规模研究     

高运安  胡宝贵  赵雪阳 《中国蔬菜》2017,1(5):67-71

农业适度规模经营是未来农业的发展方向,本文以规模经济理论为基础,通过建立Translog生产函数模型和DEA包络分析法,最终得出以农户为经营主体的北京市西瓜最优种植规模为6670m2(10亩)。除此之外,研究还发现种植户年龄及文化程度对西瓜种植具有显著影响,但随着农村人口老龄化日趋严峻,培养新型职业农民可以衔接西瓜种植的"断层"。  相似文献