小麦矮腥黑粉菌g9890基因编码效应蛋白的生物信息学分析及亚细胞定位     

许停  刘太国  高利  陈万权 《植物保护学报》2023,50(4):866-872

为明确小麦矮腥黑粉菌Tilletia controversa g9890基因编码效应蛋白的生物学功能,根据小麦矮腥黑粉病菌转录组测序结果,筛选出效应蛋白g9890,通过PCR技术获得g9890基因cDNA的全长序列,并对其进行生物信息学以及亚细胞定位分析。多种生物信息学数据库分析表明,g9890基因全长为1 038 bp（包括终止密码子）,共编码345个氨基酸,相对分子质量为37 353.32,理论等电点为5.02。g9890效应蛋白不稳定系数为15.94,疏水性指数为-0.312,是一种亲水性且稳定的蛋白。将g9890基因与pbin-GFP载体重组,利用冻融法转化至根癌农杆菌Agrobacterium tumefaciens GV3101中,将其注入烟草进行瞬时表达分析,并通过共聚焦激光显微镜观测该基因的定位状况,结果显示,g9890定位在细胞膜和细胞核上。  相似文献   

Milk Protein Polymorphism in Kangayam Cattle     

Jeichitra V  Kandasamy N  Panneerselvam S 《Tropical animal health and production》2003,35(2):147-153

This paper reports the milk protein polymorphism, the allele frequencies of variants and the possible linkages among various combinations of milk protein phenotypes in the Kangayam cattle of south India. Milk samples from 156 Kangayam cows were typed by starch gel and polyacrylamide gel electrophoresis for caseins and whey proteins, respectively. All the four milk protein components studied, g src="/content/g9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">gt;s1gt;-casein, g src="/content/g9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-casein, g src="/content/g9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-lactoglobulin and g src="/content/g9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin, exhibited polymorphism with high allele frequencies of 0.9231±0.0151 for g src="/content/g9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">gt;s1gt;-casein C, 0.9263±0.0148 for g src="/content/g9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-casein A, 0.9135±0.0159 for g src="/content/g9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-lactoglobulin B and a relatively high frequency of 0.6218±0.0275 for g src="/content/g9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin A. The mean heterozygosity estimated over all the four milk protein loci was 0.2420. Genetic equilibrium was observed among all the loci studied, except g src="/content/g9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin. Linkage analysis confirmed the non-independence between g src="/content/g9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">gt;s1gt;- and g src="/content/g9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-caseins and between caseins and g src="/content/g9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin phenotypes.  相似文献   

Crop productivity under differently lopped canopies of multipurpose trees in Central Himalaya, India   总被引：3，自引：1，他引：3  

R.L. Semwal  R.K. Maikhuri  K.S. Rao  K. Singh  K.G. Saxena 《Agroforestry Systems》2002,56(1):57-63

gt;Ficusgt; species are multipurpose trees well known by rural populations in Sahelian and Sudanian zones of Africa. Their uses are well documented but their amenability for vegetative propagation has not been extensively studied. This paper compares the rooting ability of stem and aerial root cuttings from thirteen gt;Ficusgt; species found in West Africa. It highlights the differences between species belonging to the sub-genera gt;Sycomorusgt; and gt;Urostigmagt;. The former show no capacity to propagate from cuttings whereas the latter, with epiphytic development, can be propagated by cuttings, although this capacity varies among species. Thus, gt;F. thonningii, F. leprieuriigt; and gt;F. ovatagt; are easily propagated, while gt;F. platyphyllagt; and gt;F. elasticoidesgt; are propagated with difficulty. The rooting capacity also varies depending on the cutting material used. It decreases in the following order: long leafless hardwood cuttings (pole) > nodal cuttings > apical cuttings. Rooting potential increases when the cuttings are harvested towards the end of the dry season(March to May). Aerial root can be used for cuttings in all species of the sub-genus gt;Urostigmagt;. The capacity of root cuttings to regenerate is greatest when cuttings are collected at the beginning of the dry season (November). In this case, wound-induced adventitious roots arise at the basal end of the cutting while gt;de novogt; buds are developed from the cambium at the distal end. The subsequent morphological development is identical to that of a stem cutting. These results clarify and allow the optimal use of the knowledge and methods developed by the indigenous people of the Sahel and could assist and promote fig tree (gt;Ficusgt; sp.) domestication in the dry tropics.This revised version was published online in November 2005 with corrections to the Cover Date.  相似文献   

Hormonal changes during meiotic maturation and ovulation in the brook trout (Salvelinus fontinalis)     

Frederick W. Goetz  Alexis Y. Fostier  Bernard Breton  Bernard Jalabert 《Fish physiology and biochemistry》1987,3(4):203-211

The plasma levels of estradiol-17g src="/content/g516x10847181264/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0"> (E2), 17g src="/content/g516x10847181264/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">, 20g src="/content/g516x10847181264/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-dihydroxy-4-pregnen-3-one (17,20-P) and gonadotropin (GTH) were measured in brook trout (gt;Salvelinus fontinalisgt;) during the period from the end of vitellogenesis to postovulation. Blood samples were taken according to specific stages of maturation, including germinal vesicle breakdown (GVBD) and ovulation. E2 levels were quite high (g src="/content/g516x10847181264/xxlarge8764.gif" alt="sim" align="MIDDLE" BORDER="0">45 ng/ml) at the end of vitellogenesis (and prior to GVBD) and dropped precipitously by GVBD (g src="/content/g516x10847181264/xxlarge8764.gif" alt="sim" align="MIDDLE" BORDER="0">2 ng/ml). They remained low through ovulation and postovulation. 17,20-P levels were low prior to GVBD (g src="/content/g516x10847181264/xxlarge8764.gif" alt="sim" align="MIDDLE" BORDER="0">0.7 ng/ml) and increased dramatically at GVBD (g src="/content/g516x10847181264/xxlarge8764.gif" alt="sim" align="MIDDLE" BORDER="0">148 ng/ml). The levels of 17,20-P remained high at ovulation (g src="/content/g516x10847181264/xxlarge8764.gif" alt="sim" align="MIDDLE" BORDER="0">142 ng/ml) and then dropped significantly within 24 h to approximately half of the ovulatory values. They decreased even further by 7 days postovulation. GTH levels rose gradually through GVBD and ovulation from a postvitellogenic level of approximately 3 ng/ml to a 7 day postovulatory value of approximately 10 ng/ml. The overall results; 1) decrease in estradiol prior to GVBD, 2) increase in 17,20-P at GVBD and 3) gradual GTH rise through GVBD and ovulation, are similar to those reported for other salmonids.  相似文献   

Determination of primary structure of amberjack myosin heavy chain and its relationship with structural stability of various fish myosin rods   总被引：1，自引：0，他引：1  

Reina Kawabata  Nobuyuki Kanzawa  Masahiro Ogawa  Takahide Tsuchiya 《Fish physiology and biochemistry》2000,23(4):283-294

The structural stability of fish myosin depends upon species and temperatures of water in which fish live. Primary, secondary, and quaternary structures of myosin heavy chain (MyHC) from three species of fish living at different temperature ranges have been compared with those of rabbit MyHC in order to investigate the differences in stability. Primary structure of MyHC, although being accessible for warm-water and cold-water fish (carp and walleye pollack), was not available in previous for tropical-water fish literature; so in this study primary structure of MyHC of the tropical-water fish amberjack has been determined by cloning and sequencing its cDNA. The MyHC has 1938 amino acid residues (AA), which are almost as much as as those of carp and walleye pollack. The amberjack MyHC is 91–95% homologous with other fish and rabbit MyHCs. There is a discernible difference between animal species with stable myosin rod (amberjack, carp, and rabbit) and walleye pollack with unstable rod. Stable rod species have a high probability of forming coiled-coil around the COOH-terminal end of the rod, while the pollack has a low coiled-coil formation probability. In addition, the average scores of the coiled-coil for myosin rod were rabbit (1.738) > amberjack (1.691) > carp (1.680) > walleye pollack (1.674) which correlated exactly with the observed stability. The results suggest that coiled-coil forming ability, particularly around the COOH-terminal end, directs structural stability of fish myosin rod.  相似文献   

Temporal changes in plasma thyroid hormone,growth hormone and free fatty acid concentrations,and hepatic 5′-monodeiodinase activity,lipid and protein content during chronic fasting and re-feeding in rainbow trout (Oncorhynchus mykiss)     

K. J. Farbridge  J. F. Leatherland 《Fish physiology and biochemistry》1992,10(3):245-257

Temporal changes in growth, plasma thyroid hormone, cortisol, growth hormone (GH) and non-esterified fatty acid (NEFA) concentrations, hepatic Tgt;3gt; content and hepatic 5g src="/content/t316853g28687001/xxlarge8242.gif" alt="prime" align="BASELINE" BORDER="0">-monodeiodinase activity were measured in rainbow trout (gt;Oncorhynchus mykissgt;) subjected to a sustained fast for up to eight weeks, and during a four-week re-feeding period. The purpose of the study was to examine aspects of the endocrine control of energy partitioning processes characteristic of short-term (acute; fasting) and long-term (chronic; starvation) food-deprivation states in fish, and to explore the role of the thyroid hormones, cortisol and GH in the energy repartitioning that takes place during an acute anabolic (re-feeding) state following chronic food deprivation.Differences in growth rate between fed and fasted groups were evident after two weeks, but significant weight loss by the fasted groups was not evident until between four and six weeks into the fast. Hepatosomatic indices (HSIs) were significantly reduced in the fasted fish within seven days, and as early as two days in one study; recovery of the HSI in fasted fish was evident within three days of re-feeding. Liver protein content (expressed as % wet weight) was consistently depressed in the fasted fish in only one of the three studies. Liver total lipid content (expressed as % wet weight) was depressed in the fasted fish within two days of food deprivation. Because of the rapid and sustained decrease in the HSI of fasted fish, the hepatic gt;totalgt; protein and lipid reserves, when considered on a body weight basis, were markedly lowered within the first few days of the fast. Plasma GH concentrations exhibited a bi-modal pattern of change, with a transient fall in levels, followed by a sustained increase in fasted fish. The indicators of interrenal activity were suggestive of a depressed pituitary-interrenal axis in fasted animals; plasma cortisol levels were elevated to levels of fed animals within one day of re-feeding. The indicators of thyroid hormone economy (plasma thyroid hormone levels, liver triiodothyronine content, hepatic 5g src="/content/t316853g28687001/xxlarge8242.gif" alt="prime" align="BASELINE" BORDER="0">-monodeiodinase (MD) activity, thyroid epithelial cell height) were similarly indicative of a depressed pituitary-thyroid axis in fasted animals, with recovery to levels of the fed animals within one week. Despite the compensatory changes in accumulation of reserves (as indicated by a compensatory increase in HSI), there were no apparent compensatory changes in any of the endocrine parameters evident during the re-feeding period.  相似文献   

忆晋西一次修成水平梯田的产生和兴起     

刘足征 《中国水土保持》1994,(7):54-55

忆晋西一次修成水平梯田的产生和兴起刘足征（湖南省水电厅，长沙市４１０６０７）在晋西吕梁山区以至黄河中游广大山区，现在到处可以见到层层叠叠的一次修成的水平梯田。面对这一雄伟壮观，令人心情舒畅，不禁回想到４０年以前的景象。那时，在这片黄土高原广大山区，从...  相似文献   

Effect of molecular complexity and acidity of earthworm faeces humic fractions on glutamate dehydrogenase,glutamine synthetase,and phosphoenolpyruvate carboxylase in Daucus carota α II cells     

A. Muscolo  M. R. Panuccio  M. R. Abenavoli  G. Concheri  S. Nardi 《Biology and Fertility of Soils》1996,22(1-2):83-88

Carrot cells were grown in cultures supplemented with two hormones [2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (6BAP)] and two humic fractions extracted from earthworm faeces, one with high acidity and a low apparent molecular size (<3500) and the other with low acidity and a large molecular size. 2,4-D stimulated growth through an effect on cell enlargement, while the strongly acidic humic fraction (0.2 mg lgt;-1gt;) and the weakly acidic fraction (1 mg lgt;-1gt;) were both less effective. With 4–16 h of pre-incubation, the highly acid humic fraction, mainly alone, induced the best increase in protein content; the effect of the weakly acid humic fraction and the hormones was generally less important. The two humic fractions also differed in their influence on glutamate dehydrogenase activity. After 2 h of pretreatment, the highly acidic fraction increased glutamate dehydrogenase activity, while the other fraction did not affect it. After 4–16 h of pre-incubation, the activity of this enzyme was still not influenced by these humic fractions. The presence of the two hormones did not interfere with the humic matter effects. Glutamine synthetase activity was not affected by a pre-incubation of up to 4 h with the two humic fractions, but it was stimulated after 8–16 h of pre-incubation. A 2,4-D+6BAP mixture stimulated glutamine synthetase activity (from +12 to +50%). Again, the presence of the hormones did not interfere with the effects induced by the humic fractions. After 16 h of pre-incubation, phosphoenolpyruvate carboxylase activity was increased by the highly acidic humic fraction (+93%) and by both humic fractions together (+34%). An explanation of the different incubation times necessary for the humic fractions to exert stimulatory effects on these enzymes is proposed here. The regulatory properties of the strongly acidic humic fraction appeared to depend on the combination of high acidity (expecially carboxylic C) with low molecular size.  相似文献   

ANSYS在模拟桩土接触中的应用     

王先军  周文宇  蒋鑫 《森林工程》2006,22(3):49-51

建立了一个桩土接触面体系的有限元模型，并对其计算理论进行分析；结合某大直径钻孔灌注桩工程实例，分析了桩土模型的荷载-沉降曲线及荷载的传递规律：通过与桩基静载试验数据对比，得出有限元模拟结果与实测值相近。分析结果对以后桩土模拟及设计有重要参考价值。  相似文献   

Impact of let-7g on Proliferation and Lactation of Mouse Mammary Epithelial Cells     

Feng Li  Li Qing-zhang  Cui Wei  Ding Wei 《东北农业大学学报(英文版)》2012,19(3):67-71

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