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Five horses presented with unilateral pink, smooth, nonulcerated conjunctival masses with histologic features characteristic of inflammatory pseudotumors, i.e. proliferative inflammatory lesions clinically resembling true neoplasia. Although causes for the inflammatory lesions were not determined, based on the presence histologically of mononuclear (predominantly lymphocytic) inflammatory cell infiltrates and the absence of infectious agents, parasites or foreign bodies, an immune-mediated pathogenesis was suspected. Affected horses ranged from 5 to 8 years of age with no apparent breed or sex predilection. Conjunctival lesions were nodular in two cases and relatively flat and more diffuse in three cases. Third eyelid lesions were present in three cases and two affected eyes had corneal involvement. Based on findings from these five cases, the prognosis for equine conjunctival pseudotumors appears to be good when lesions are treated by partial or complete surgical excision, local administration of anti-inflammatory agents, or a combination of surgery and anti-inflammatory therapy. 相似文献
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旨在比较ALV-J-SD1005毒株和ALV-J-NX0101毒株感染鸡只致病性、诱发先天性免疫因子和致肿瘤因子表达的差异,用感染剂量为103TCID50的两株病毒分别颈部皮下接种75只1日龄海兰褐鸡,感染后7、14、21 d检测体重、肿瘤病变、死亡率、血液和皮下纤维组织中病毒含量以及肝中鸡TRIM25、MDA5、IRF7、IFN-α/β、14-3-3σ、P53、STAT1等免疫因子或肿瘤因子的mRNA表达量。结果显示,雏鸡感染ALV-J-SD1005毒株后最早于第10天出现纤维组织增生,14 d致纤维组织增生率为100%(18/18),死亡率为5.2%(1/19);随着感染日龄的增加,增生组织指数和死亡率不断升高,21 d时分别达34.4%(74.5/209.5)和58.3%(7/12)。血液和皮下纤维组织的病毒载量显著升高;同时,显著上调鸡肝中MDA5、IRF7、P53等基因的表达量,下调IFN-α/β和14-3-3σ基因的表达量;而鸡TRIM25基因呈现感染早期(7 d)表达显著下调,后期(14~21 d)表达显著上调。ALV-J-NX0101毒株感染后21 d未检测到肿瘤发生,也没有鸡只死亡,但见鸡血液等组织中病毒载量显著增多,鸡TRIM25、MDA5、IRF7、IFN-β、IFN-α基因表达显著下降,STAT1基因表达显著上调。上述结果可以看出,ALV-J-SD1005毒株与ALV-J-NX0101毒株在感染鸡体内诱发不同的抗病毒反应和抗肿瘤反应,导致产生明显不同的病毒增殖和致病特点。本研究为深入理解两株ALV-J病毒致肿瘤机制、探索新诊断标识提供重要科学依据。 相似文献
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Jie Liu Rumi Fujita Michiko Sato Kuniyoshi Shimizu Fumiko Konishi Kiyoshi Noda Shoichiro Kumamoto Chie Ueda Hisatoshi Tajiri Shuhei Kaneko Yoshitaro Suimi Ryuichiro Kondo 《Journal of Wood Science》2005,51(2):189-192
The inhibitory effects of 102 methanol extracts of 40 mycelia, 9 culture fluids, and 53 fruiting bodies of 40 strains of Ganoderma lucidum on 5-reductase were investigated. The methanol extract of the fruiting body of each strain was found to show the strongest 5-reductase inhibitory activity among the extracts tested. 相似文献
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Amoebic gill disease (AGD) has been attributed to infection by Neoparamoeba sp. The causal mechanisms for AGD lesion development and the primary pathogenic role of Neoparamoeba sp. require elucidation. Three groups of Atlantic salmon were exposed to viable gill isolated amoebae, to sonicated amoebae, or to sea water containing viable amoebae without direct contact with gill epithelia. Fish were removed 8 days post-exposure and the gills assessed histologically for AGD. AGD occurred only when fish were exposed to viable trophozoites. Consequently, in an accompanying experiment, infection was evaluated histologically at 12, 24 and 48 h post-exposure in three groups of salmon, one group being mechanically injured 12 h prior to exposure. A progressive host response and significant increase (P < 0.001) in the numbers of attached amoebae was apparent over the 48-h duration in undamaged hemibranchs in both treatment groups. There were no significant differences to mucous cell populations. Attachment of Neoparamoeba sp. to damaged gill filaments was significantly reduced (P < 0.05) by 48 h post-exposure. These data further confirm and describe the primary pathogenic role of Neoparamoeba sp. and the early host response in AGD. Preliminary evidence suggests that lesions resulting from physical gill damage are not preferentially colonized by Neoparamoeba sp. 相似文献
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Amoebic gill disease (AGD) affects the marine culture phase of Atlantic salmon, Salmo salar L., in Tasmania. Here, we describe histopathological observations of AGD from smolts, sampled weekly, following transfer to estuarine/marine sites. AGD was initially detected histologically at week 13 post-transfer while gross signs were not observed for a further week post-transfer. Significant increases (P < 0.001) in the proportion of affected gill filaments occurred at weeks 18 and 19 post-transfer coinciding with the cessation of a halocline and increased water temperature at the cage sites. The progression of AGD histopathology, during the sampling period, was characterized by three phases. (1) Primary attachment/interaction associated with extremely localized host cellular alterations, juxtaposed to amoebae, including epithelial desquamation and oedema. (2) Innate immune response activation and initial focal hyperplasia of undifferentiated epithelial cells. (3) Finally, lesion expansion, squamation-stratification of epithelia at lesion surfaces and variable recruitment of mucous cells to these regions. A pattern of preferential colonization of amoebae at lesion margins was apparent during stage 3 of disease development. Together, these data suggest that AGD progression was linked to retraction of the estuarine halocline and increases in water temperature. The host response to gill infection with Neoparamoeba sp. is characterized by a focal fortification strategy concurrent with a migration of immunoregulatory cells to lesion-affected regions. 相似文献
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Erik R. Wisner D V M Dominique Penninck DVM David S. Biller DVM Edward C. Feldman DVM Christiana Drake PhD Thomas G. Nyland DVM 《Veterinary radiology & ultrasound》1997,38(6):462-466
The purpose of this study was to assess the diagnostic utility of parathyroid ultrasonography to differentiate causes of hypercalcemia in dogs. We analyzed qualitative and quantitative ultrasound imaging findings and clinical pathology data from 33 dogs that underwent parathyroid ultrasound examination as part of the diagnostic evaluation for hypercalcemia. Diagnoses of the diseases causing hypercalcemia included parathyroid carcinoma (n = S), parathyroid adenoma (n = 15), parathyroid adenomatous hyperplasia (n = 6), chronic renal insufficiency (n = 3), and hypercalcemia of malignancy (n = 4). All parathyroid lesions were round or oval and hypoechoic compared with surrounding thyroid parenchyma. Adenomatous and adenocarcinomatous glands were 4 mm or larger in longest linear measurement on US examination and were statistically significantly larger than hyperplastic glands. (p < 0.001) Linear measurements of parathyroid glands acquired at the time of ultrasound examination correlated well with direct siz determination after surgical excision. (r2 = 0.9, p < 0.0001) Parathyroid lesions <4 mm are highly suspicious for parathyroid adenoma or carcinoma, while US lesions <4 mm most likely represent primary adenomatous hyperplasia or secondary parathyroid hyperplasia. Parathyroid size estimation from ultrasound examination is an accurate predictor of true size. 相似文献
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观察四种联合止血法摘除前列腺的止血效果。采用术前静滴5-FU,术中在3,5,7,9点缝扎前列腺动脉,腺体内注射无水酒精,气囊压迫四种联合止血。结果;31例主出血量为50-300ml,平均为75ml。结论:联合止血法摘除前列腺止血效果良好。 相似文献