乳猪体内糖类的消化和吸收研究     

刘文宗  李鑫  闫长亮  包海生  甘潇  李军 《四川畜牧兽医》2004,31(12):31-32

乳猪小肠内葡萄糖的分布状况既与饲料的碳水化合物种类和数量相关，也与小肠的不同部位相关，十二指肠段和空肠段分别是乳糖、淀粉类碳水化合物分解和葡萄糖吸收的主要部位血糖浓度离肝脏越远含量越低，而与饲料中糖类含量的多少无关。  相似文献   

Roles of spermine in modulating the antioxidant status and Nrf2 signalling molecules expression in the thymus and spleen of suckling piglets—new insight          下载免费PDF全文

G. Jia  H. Zhao  X. Chen  C. Wu  J. Tang  J. Wang  J. Cai  G. Liu 《Journal of animal physiology and animal nutrition》2018,102(1):e183-e192

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参金止痢口服液对腹泻哺乳仔猪肠道微生物菌群影响的差异性分析     

涂腾  丁俊仁  李思聪  魏勇  李旭廷  梁歌  杨锐 《中国兽药杂志》2021,55(6):35-44

研究参金止痢口服液对腹泻哺乳仔猪肠道微生物菌群的影响。选取同等饲养条件下7~15日龄体重相近的“杜×长×大”三元杂交仔猪共计9头,其中有腹泻症状的6头将其随机平均分为腹泻空白组(FXK)和腹泻中药组(FZ),正常的3头为健康空白组(JK),中药组灌服参金止痢口服液,空白组灌服同等体积生理盐水,连续灌胃5d,在给药后的第8天对仔猪采血进行抗体及细胞因子水平测定,并采集结肠内容物提取样本中菌群总DNA进行16S rRNA高通量测序。结果显示：与FXK组相比,FZ组血清中IgG含量显著升高(P＜0.05),IgM含量显著降低(P＜0.05),炎性因子IL-1β、IL-6、TNF-α含量显著下调(P＜0.01)。α、β多样性分析结果表明,三个组结肠肠道菌群结构差异显著。在门分类水平上,FZ组仔猪肠道拟杆菌门相对丰度显著高于FXK组(P＜0.05),变形菌门相对丰度显著低于FXK组(P＜0.05),而FZ与JK组之间两个菌门差异不显著。属水平上,FZ组韦荣球菌属和乳杆菌属相对丰度显著低于FXK组(P＜0.05),普雷沃氏菌属相对丰度显著高于FXK组(P＜0.05),FZ组与JK组之间这三个菌属差异不显著。综上结果表明,参金止痢口服液可能通过调节免疫及炎性相关因子水平和调节肠道菌群结构从而发挥治疗哺乳仔猪腹泻的作用。  相似文献   

兔出血症病毒人工感染幼兔的病理学研究     

黄吉风  徐福南 《南京农业大学学报》1997,20(2):77-81

用兔出血症病毒（ＲＨＤＶ）分别感染５，１５和２５日龄家兔，接种后７２ｈ扑杀，对其肝，脾，肺，肾，胸腺，骨髓等作组织病理学和超微结构观察，发现幼兔感染ＲＨＤＶ与成年兔病毒性出血症不同，其主要病变是血管内皮细胞，网状细胞的增生和肝，肾等脏器实质细胞的变性，ＲＨＤＶ对一月龄以内幼兔抗亦具有感染性，并对其肝，肾等造成一定程度的病理损伤，病理损伤程度随年龄的增长而加重。  相似文献   

Analysis of the miRNA Expression Profiles in the Primary Neurons of Mice Infected with Japanese Encephalitis Virus     

DU Jiaru  CHEN Haonan  WANG Fangyu  ZHANG Yunshang  FAN Jianming 《畜牧兽医学报》1956,51(9):2319-2323

The interaction between JEV and the host at the miRNAs level was preliminarily explored by studying the miRNAs expression profiles of primary neurons in mice infected with JEV. Total RNA of JEV-infected and uninfected primary neurons of the suckling mice was extracted individually by Trizol and then analyzed miRNA expression profiles by high-throughput sequencing analysis. Significantly differentially expressed miRNAs were selected for verification by real-time quantitative PCR. Through bioinformatics analysis, 26 miRNAs with significant expression differences were screened out, among which 18 miRNAs were up-regulated and 8 miRNAs were down-regulated. The results of quantitative real-time PCR of the JEV-E gene indicated that the expressions of mir-21a-3p mir-223-5p mir-147-3p mir-155-5p and mir-146a-5p could promote the expression of JEV-E gene in neurons, while the expression of mir-301a was just on the contrast. The expression of miRNAs in primary neurons could affect the replication of JEV. This study provided the theoretical basis and direction for further studies on the regulatory function of miRNAs in the mechanism of neural dysfunction induced by JEV.  相似文献   

Maternal low-protein diet affects bone mass and mineral metabolism in suckling rats     

Fetoui H  Mahjoubi-Samet A  Guermazi F  Zeghal N 《Journal of animal physiology and animal nutrition》2008,92(4):448-455

This study was performed to analyse the effects of low-protein diet (7%) on bone mass and mineral metabolism in rat pups whose mothers were submitted to protein-restricted diet during late pregnancy and early post-natal periods. For this purpose, 12 pregnant Wistar rats were divided into two groups of six animals each: a control group with free access to standard diet (20% protein) and protein-restricted group (PR) fed with low-protein diet (7% protein) from the 14th day of pregnancy until day 14 after delivery. Body weight, femur bone mass, plasma thyroid hormones (FT₄ and FT₃), biochemical bone marker levels [alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (ACP)] and bone DNA content were recorded. In undernourished pups, a decrease in body weight (−47%, p < 0.001) in skeletal growth (−11%, p < 0.001) and in bone DNA content (−48%, p < 0.001) were observed. Plasma protein and albumin levels and thyroid status were also decreased in undernourished rat pups compared with those of control group. The circulating amino acid levels were decreased in pups. While in their mothers, some of them were increased and the others were decreased. A positive correlation was observed between bone mass and plasma thyroid hormone levels and ALP activity and plasma protein levels, and a negative correlation with ACP activity. Plasma ALP levels were decreased by 19%, whereas plasma tartrate-resistant ACP levels were increased by 33% indicating a hyperactivity of bone remodelling. These results showed that protein-restricted diet given to mothers during late pregnancy and early post-natal periods induced changes in body weight, skeletal growth and bone metabolism in their offspring.  相似文献   

哺乳期幼驼与成年母驼粪便菌群多样性分析     

王家豪  王静  袁鹏  李志伟  耿娟  王卉  李凤鸣  臧长江 《中国畜牧兽医》2023,50(1):97-105

【目的】探究准噶尔双峰驼哺乳期幼驼和成年母驼粪便菌群结构组成及多样性变化,为幼驼肠道健康发育提供重要依据。【方法】选取6～7岁体况相近的健康成年母驼12峰,以及出生日期相近(3月龄)的哺乳期母幼驼12峰,母驼与幼驼在相同饲养环境下饲养。采用直肠取粪法采集粪样,用于粪便内容物细菌16S rDNA的V3-V4区测序,并对测序数据进行相应分析。【结果】母驼粪便菌群Chao1和Shannon指数均显著高于幼驼(P<0.05);在门水平上,母驼和幼驼粪便中位于前十的菌均为厚壁菌门、拟杆菌门、疣微菌门、变形菌门、软壁菌门、螺旋体门、TM7、放线菌门、蓝藻菌门和纤维杆菌门,其中幼驼粪便中变形菌门和软壁菌门丰度均极显著高于母驼(P<0.01);在科水平上,母驼与幼驼粪便中位于前十的菌均为瘤胃球菌科、消化链球菌科、毛螺菌科、梭菌科、克里斯滕森菌科、理研菌科、疣微菌科、拟杆菌科、艰难杆菌科和普雷沃氏菌科,其中母驼粪便中消化链球菌科和梭菌科的丰度均极显著高于幼驼(P<0.01),而幼驼粪便中毛螺菌科的丰度极显著高于母驼(P<0.01);在属水平上,母驼与幼驼粪便中位于前十的菌属均为梭...  相似文献   

哺乳期灌服亚精胺或精胺对早期断奶大鼠生长及肠道发育的影响   总被引：1，自引：0，他引：1  

陶青燕  王康宁 《中国畜牧杂志》2009,45(9)

为考察断奶前多胺诱导的肠道成熟是否具有缓解早期断奶应激的作用,本研究选取同窝14d清洁级SD哺乳大鼠分别经胃管连续3d灌服5μmol亚精胺,5μmol精胺或生理盐水(对照组)。17d实施早期断奶,一半幼鼠断奶后立即致死,剩余一半独立饲养至20d后致死,测定幼鼠体重及肠道发育指标。结果表明:亚精胺和精胺促进了17d哺乳大鼠肠道成熟,表现为肠道重量、肠长、小肠蛋白质、蔗糖酶活性的升高。亚精胺和精胺可有效阻止早期断奶失重,并降低或消除了早期断奶肠道的应激反应,表现为肠道重量、肠长、肠道蛋白质、肠道DNA和蔗糖酶、乳糖酶活性在断奶前后不受影响。亚精胺和精胺对肠道杯状细胞类型有显著影响,断奶后酸性杯状细胞分别增加20.1%(P>0.05)和89.5%(P<0.05)。综上可得出,断奶前灌服亚精胺或精胺具有促进哺乳期肠道成熟,降低早期断奶应激和增强黏液屏障的作用。  相似文献   

EM菌液对乳猪黄痢的防治效果   总被引：2，自引：0，他引：2  

路彩霞  李军成  何尚君 《安徽农业科学》2009,37(22):10516-10517

[目的]研究EM菌液对乳猪黄痢的早期防治效果。[方法]将14头母猪中每2头母猪所产的22头乳猪分为1组（共6个试验组和1个对照组）,试验组乳猪出生后分别灌胃EM原露3、5my头,发生黄痢后灌服EM原露5、10、20mL／头,对照组乳猪出生后不灌服EM原露,发病后灌服痢菌净;在饲料中掺入20mlEM原露饲喂母猪,观察所产乳猪的发病情况。[结果]灌胃试验中,试验组乳猪总发病率为6．06％,对照组乳猪发病率为40．91％,发病后灌服5mlEM原露有1头死亡、灌服10、20mlEM原露无死亡现象;在饲料中掺入20mlEM原露饲喂母猪后,所产乳猪3d内的总发病率为16．92％,对照组发病率为49．62％。[结论]EM菌液可有效防治乳猪早期黄痢。。  相似文献   

饲粮添加中药渣和发酵中药渣对母猪繁殖性能与子代发育的影响     

李华伟  黎智华  祝倩  吴灵英  印遇龙  孔祥峰 《动物营养学报》2017,29(1)

本试验旨在通过研究微生物发酵中药渣对围产期母猪繁殖性能和子代发育的影响,为中药渣的二次开发利用提供依据。试验选用2~4胎次、预产期相近的妊娠85 d的健康二元母猪60头,随机分为3组,每组20头。3组分别在基础饲粮中添加2 kg/t米糠(对照组)、2 kg/t中药渣制剂(由黄芪、当归、熟地黄和白芍等组成,中药渣组)和2 kg/t发酵中药渣制剂(发酵中药渣组)。从产前21 d开始饲喂,到产后21 d结束。分别于妊娠110 d、产后21 d时测量母猪背膘厚,记录母猪的产仔数、产活仔数、死胎数、木乃伊数和母猪产后发情间隔;记录仔猪初生窝重,7、14和21日龄窝增重,并计算平均日增重;每天观察仔猪腹泻情况,并计算腹泻率。结果表明:与对照组相比,发酵中药渣组仔猪断奶窝增重显著增加(P0.05),中药渣组和发酵中药渣组仔猪1~14日龄、1~21日龄腹泻率显著增加(P0.05);与中药渣组相比,发酵中药渣组仔猪7日龄窝增重显著增加(P0.05),平均日增重呈升高趋势(P=0.053)。由此可见,围产期母猪饲粮添加中药渣制剂虽然造成哺乳仔猪一定程度的腹泻,但可促进其生长发育,添加发酵中药渣制剂效果更好。  相似文献