生长抑素(SS)与硫氧还原蛋白(Thioredoxin)相融合生成的2种基因工程化融合蛋白SS-N/X和SS-N/S的表达特性   总被引：1，自引：0，他引：1  

刘永庆  陈溥言  杜念兴  赵国屏 《中国兽医学报》2003,23(2):172-176

对基因工程化生长抑素（somatostatin,SS)-硫氧还原蛋白（Thioredoxin)融合蛋白SS－N／X和SS－N／S的表达产量、水溶性、Triton X-100对SS融合蛋白水溶性的影响、包涵体的溶解和复性特点以及SS的抗原性与免疫原性等特性进行了研究。结果显示，在30℃的低温下用IPTG诱导表达，SS－N／X融合蛋白主要以可溶性形式存在，占75．8％，色涵体仅占24．2％；而SS－N／S融合蛋白则主要以包涵体的形式存在，占81．1％，可溶性蛋白仅占18．9％；32C载体（pEG-32C)蛋白则居二者之间，可溶性蛋白与包涵体大约等比例出现。SS－N／S在低温下诱导可超量表达SS融合蛋白，其表达量约占菌体总蛋白的40．94％。0．5％ Triton X-100对融合蛋白的不溶性有非常显著的影响，几乎可使所有的SS－N／X融合蛋白和大部分SS－N／S融合蛋白及32C硫氧还原蛋白变成水溶性的。32C硫氧还原蛋白包涵体在尿素中的溶解度明显高于SS－N／S包涵体，2mol/L尿素可使近一半的32C硫氧还原蛋白包涵体溶解，而SS－N／S包涵体只有27．5％溶解；但在5mol/L尿素时，两者均可基本全部变为可溶性的。在SS融合蛋白包涵体溶解时加入还原剂二硫苏糖醇，可明显降低SS的抗原性。SS融合蛋白（水溶性融合蛋白和复性后的包涵体）具有良好的SS抗原性和免疫原性。由SS融合蛋白与弗氏不完全佐剂制成试验用疫苗，免疫BALB／c小鼠、仔猪和羔羊等动物，可显著提高免疫动物的增重。  相似文献   

兔出血症-巴氏杆菌病铝胶二联灭活苗的研制     

李元新  刘来利  王必成 《畜牧兽医杂志》2011,30(5):12-15

筛选了具有良好免疫原性的兔瘟强毒株GMH881和兔巴氏杆菌C51-17株,研制出了兔出血症-巴氏杆菌二联铝胶灭活苗,对疫苗进行了免疫剂量、免疫产生期、兔出血症抗体测定、免疫保护期、保存期等试验;确定出二联铝胶苗免疫剂量1.0 mL;二联铝胶苗在免疫后7 d对兔病毒性出血症强毒的保护率达到100%,二联苗免疫后10～14 d对兔巴氏杆菌的保护率均达到75%以上;二联铝胶苗的兔病毒出血症抗体测定结果表明：二联铝胶苗免疫家兔后7 d,HI抗体均可达25.0,15 d明显上升,二联苗HI效价60～90 d达到最高峰,最高可达210,二联苗在120～180 d仍可维持在27.0～29.5水平。二联铝胶苗分别在免疫后4个月、6个月用兔出血症强毒攻击均产生100%保护,在6个月用2个MLD的兔巴氏杆菌攻击保护率可达70%以上,二联铝胶苗4～8℃条件下保护期暂定为1年,25℃条件下暂定为半年。  相似文献   

新型国产白油佐剂与进口Marcol-52白油佐剂配制疫苗的比较试验     

尹秀凤  丁美娟  黄显明 《中国畜牧兽医》2014,41(3):245-247

The Newcastle disease inactivated vaccines were prepared with oil adjuvants respectively from domestic and import.The experiments were done in order to compare the new domestic oil and Marcol-52 according to a series of testing indexes. The results showed that the quality of the new domestic oil was better than Marcol-52, except the absorptive quality.  相似文献   

猪圆环病毒2型灭活疫苗中病毒抗原含量实时荧光定量PCR检测方法的建立     

李雪峰  康斌  赵炳武  武玉梅  戴伶俐  董鹏  张建春  张春阳  斯琴高娃 《畜牧与饲料科学》2021,42(1):8-13

依据GenBank公布的猪圆环病毒2型Cap基因序列,在保守区域设计特异性引物和TaqMan探针,优化反应体系,建立评价猪圆环病毒2型灭活疫苗中病毒含量的实时荧光定量PCR检测方法,对方法的特异性、敏感性和重复性进行试验,并验证灭活剂用量和灭活时间对检测结果的影响。结果显示：该方法只对猪圆环病毒2型基因有特异性扩增,其他3种对照病毒基因的扩增结果均为阴性;检测灵敏度达到10^2.0 TCID₅₀/mL,比普通PCR方法高100倍;方法的重复性好,对同一样品进行10次检测,变异系数为2.28%;不同灭活剂用量和灭活时间对结果的影响较小,不会因各厂家使用的灭活剂用量和灭活时间不同,影响疫苗对比实验的公平性。该研究成功建立了一种评价猪圆环病毒2型灭活疫苗中病毒含量的实时荧光定量PCR检测方法,用于猪圆环病毒2型灭活疫苗样品中病毒抗原含量的定量,其结果可以反映不同猪圆环病毒2型灭活疫苗样品中抗原含量差异,为研究猪圆环病毒2型灭活疫苗病毒抗原含量评估方法提供了新的思路。  相似文献   

五种市售鸡传染性鼻炎灭活疫苗免疫效力比较     

孙惠玲  苗得园  陈晓峰  张培君  龚玉梅  唐桂运  Anne-Lise  Saint-Ger 《中国预防兽医学报》2005,27(6):544-548

本研究对国内市场上几个主要鸡传染性鼻炎灭活疫苗生产厂家生产的灭活疫苗免疫鸡只进行血清HI抗体水平测定和攻毒,比较不同公司所产疫苗效力的效力差异,并评估A、C型二价灭活疫苗两次免疫鸡群对国内B型分离株:DL-1株和最近从免疫失败鸡场分离到的SD-1株的交叉保护作用,分析免疫失败的原因.结果表明:A、D、E公司的疫苗免疫两次后,A型HI抗体阳性率分别为92.5%、100%和95%,滴度分别为33.9、55.1和59.9,攻毒保护率都是100%.C型HI抗体阳性率分别为72.5%、38.5%和77.5%,滴度分别为11.4、2.7和27,攻毒保护率分别为80%、70%和80%.而B和C公司的疫苗免疫两次后A型HI抗体阳性率分别为59.4%、77.1%,抗体滴度分别为5.4和21.8,攻毒保护率分别为50%和66.7%;C型HI抗体阳性率分别为54.1%和51.4%,抗体滴度分别为6.1和6.8,攻毒保护率分别为38.3%和50%.在五个疫苗产品中,以A、D、E的保护效力较好,B、C产品效力较差.另外,A、C型二价灭活疫苗免疫后不能对B型菌的攻击提供保护,其A、C型HI抗体阳性率、抗体滴度与对B型菌攻毒保护率无相关性.  相似文献   

Anticoccidial Vaccination of Broiler Chickens in Various Management Programmes: Relationship between Oocyst Accumulation in Litter and the Development of Protective Immunity     

Williams RB  Johnson JD  Andrews SJ 《Veterinary research communications》2000,24(5):309-325

Paracox anticoccidial vaccine was administered to a 7-day-old flock of commercial broiler breeder stock subsequently reared to point-of-lay in the same house. For comparison, three subgroups of another flock of broiler breeders were also vaccinated with Paracox at 7 days of age, reared to 42 days and then transferred to new litter on another farm until point-of-lay. The first subgroup received no further treatment, but the second and third each received a second vaccination with Paracox, either immediately after transfer to the new litter or 42 days after transfer. Using an Eimeria necatrix model, protective immunity was demonstrated by virulent challenge of samples of birds from all groups by the age of 37–40 days (30–33 days after the first vaccination), and was maintained to at least 122–125 days of age, whether the birds remained on the same litter or were transferred to another farm, and whether they received one or two anticoccidial vaccinations. Therefore, there is no disadvantage in transferring birds onto new litter 35 days after a single Paracox vaccination, nor is there any advantage in giving a second vaccination after such a transfer. Vaccinated birds seeded the new litter with oocysts, despite being clinically immune to coccidiosis. A supplementary laboratory experiment showed that birds vaccinated at 8 days of age passed almost no oocysts after a second vaccination at 43 days of age. This indicated that they were not only protected against clinical coccidiosis, but were almost solidly immune to a homologous infection 5 weeks after a single vaccination. Nevertheless, oocysts appeared in the litter of all four groups of commercial breeders throughout the trial, showing that wild-type heterologous infections occurred whether the birds were transferred to new litter or not, but these did not overwhelm the acquired protective immunity and cause clinical coccidiosis.  相似文献   

不同鸡新城疫疫苗免疫鸡血清HI抗体的测定   总被引：3，自引：0，他引：3  

杨百亮  吕慧强 《中国畜牧兽医》2006,33(5):53-55

将试验鸡分成3个试验组和1个对照组。A组鸡接种鸡新城疫系疫苗,B组鸡接种油乳剂灭活苗,C组鸡接种鸡新城疫系疫苗,并在接种疫苗后第3、4、5、6、7、9、11、13、15、20、25d采取各组鸡血并分离血清,检测HI抗体。结果表明,接种系疫苗的组,HI抗体效价均值从4.67log2上升到10log2,接种后第5d开始上升,接种后第11d达到峰值,持续6d保持高滴度抗体水平。接种系疫苗的组,HI抗体效价均值从4.67log2上升到7log2,接种后第4d开始上升,接种后第9d达到峰值。接种油乳剂灭活苗的组,HI抗体效价均值从4.67log2上升到9.33log2,接种后第5d开始上升,接种后第11d达到峰值,持续16d保持高滴度抗体水平。系疫苗HI抗体效价上升快,效价高,较适合于紧急接种,油乳剂灭活苗HI抗体效价可在高水平维持较长时间,较适合于预防接种。  相似文献   

日本血吸虫候选疫苗免疫保护力研究进展     

黄复深  伍小松  陈尔曼 《中国兽医寄生虫病》2007,15(4):39-44

血吸虫病是危害严重的寄生虫病之一,其疫苗的研究是血吸虫病防治的一项工作重点也是一大难题。本文综述了日本血吸虫疫苗侯选分子筛选、模拟表位疫苗和多价疫苗的研究现状,并对血吸虫疫苗的深入研究进行了分析。  相似文献   

Etiology and immunoprophylaxy problems in sheep foot-rot (author's transl)]     

R V Katitch 《Comparative immunology, microbiology and infectious diseases》1979,2(1):55-59

The different aspects of the foot-rot were considered. The author described the foot-rot as a secondary polymicrobic infection due to pre-existent lesions of the hoof.The role of the different pathogenic germs was surveyed in terms of their relationship to Sphaerophorus necrophorus.Vaccination trials with different types of vaccines were analysed.In conclusion, polyvalent vaccines prepared with strains identified in the field should be preferred.  相似文献   

Assessment of the potential relationship between egg quality and infectious bronchitis virus infection in Australian layer flocks     

KA Hewson  T Robertson  PA Steer  JM Devlin  AH Noormohammadi  J Ignjatovic 《Australian veterinary journal》2014,92(4):132-138

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