NO对大鼠中脑腹侧被盖区DA神经元的调节     

孙碧英  张文慧 《动物医学进展》2003,24(4):66-68

实验观察了微量注射 NOS抑制剂 L -NAME及微量联合注射 NO的前体 L-精氨酸( L-Arg) L-NAME于大鼠中脑腹侧被盖区( VTA)对该部位多巴胺神经元的调节。发现VTA注射 L -NAME( 1 mg/ 5μL )后 ,伏隔核( Acb)多巴胺 ( DA )代谢产物—双羟苯乙酸( DOPAC)水平升高到注射前的 1 2 2 .5% ( P <0 .0 0 1 ) ,小剂量注射 L-NAME( 0 .2 mg/ 5μL)对伏隔核 DOPAC水平无明显影响 ;同样方法联合注射 L-Arg( 3 0 0 μg/ 5μL) L-NAME( 1 mg/5μL)后 ,伏隔核 DOPAC水平无明显变化。结论 :VTA微量注射 L-NAME兴奋了该部位的DA神经元 ,而 L -Arg L -NAME联合注射 ,却不能影响 DA神经元的活动 ,说明 NO可以通过L-Arg-NOS-NO途径参与 VTA多巴胺神经元的调节  相似文献   

北京鸭心迷走节前神经元的定位——HRP法研究     

陈耀星 《中国农业大学学报》1991,(3):118-121

本研究用 HRP 逆行追踪法对北京鸭心脏各区的迷走节前神经元胞体进行定位。标记细胞呈双侧性,集中在疑核和迷走背核,以疑核占优势(占68.4%)。在疑核,标记细胞多位于闩的嘴侧(峰值在+1000～+1800 μm水平),为较大型(φ20～30 μm)、多突起的星形细胞。在迷走背核,标记细胞呈梭形(φ18～20μm),大部分位于闩嘴懊I平面(+100～+1800μm)的腹侧部。左心室主要接受疑核支配。发自迷走背核的心迷走神经节前纤维主要分布在冠状沟。  相似文献   

Ginkgo biloba extract enhances c-jun expression and attenuates motoneuron death induced by root avulsion     

LI Fang-lan  ZHOU Li-hua  YUAN Qun-fang  YAO Zhi-bin  WU Wu-tian 《园艺学报》2004,20(10):1783-1785

AIM: To investigate the influence of Ginkgo biloba extract (EGb761) on c-jun expressions and motoneurons survival following root avulsion. METHODS: One hundred and eighty adult Sprague-Dawley female rats were randomly divided into control and EGb761 groups. Immediately after avulsion of C5-T1 nerve roots, the rats were injected ip with either 1 mL of EGb761 25 mg·kg^-1·d^-1 or the same volume of normal saline, and the treatment repeated everyday. At 4 h to 6 weeks following avulsion, the C7 spinal segments of all rats were collected and prepared for c-jun immunocytochemistry and neutral red stain. The numbers of c-jun positive and survival motoneurons were counted and compared between two groups at each time point. RESULTS: In control rats following avulsion, c-jun positive motoneurons appeared at 4 h, reached its maximum at 1 d and declined to 2 weeks. Avulsion-induced motoneurons death started at 2 weeks, climbed to its maximum at 4 weeks-6 weeks. In EGb761 treated rats, both numbers of c-jun positive and survival motoneurons were more than that in control group at each time point. CONCLUSION: EGb761 attenuates avulsion-induced motoneurons death, and this effect may be related to up-regulation of c-jun gene in avulsed motoneurons.  相似文献   

大脑皮质神经元培养中胰蛋白酶消化分离技术的探讨   总被引：1，自引：0，他引：1  

白瑜  赵德明 《中国畜牧兽医》2007,34(6):105-107

为探讨大脑皮质神经元培养中胰蛋白酶消化分离法的最佳条件, 取新生大鼠大脑皮质组织，在不同的胰蛋白酶消化条件下分离培养神经元，通过细胞形态观察以及染色计数比较不同消化条件对神经元活性的影响。结果表明，0.25％胰蛋白酶，37℃，5 min消化分离的神经元活性较好，细胞产率也较高。在新生大鼠大脑皮质神经元培养时，采用0.25％胰蛋白酶，37℃，5 min可消化分离到单层、生长活性较好的神经元。  相似文献   

Influences of coculture with bone marrow mesenchymal stem cells on dopaminergic neurons in brain slice treated with 1-methyl-4-phenylpytidinium     

JIN Li-rong  HONG Zhen  ZHONG Chun-jiu  WANG Yang 《园艺学报》2007,23(4):729-733

AIM: To study the protective effects of bone marrow-derived mesenchymal stem cells on damaged dopaminergic neurons induced by 1-methyl-4-phenylpytidinium(MPP+).METHODS: The parkinson disease(PD) models were established in newborn rats. Bone marrow mesenchymal stem cells(MSCs) obtained from adult bone marrow were cultured, isolated and purified. MSCs were co-cultured with brain slice and the immunohistochemical technique, electron microscopy, propidium iodide staining were used to observe the changes of neurons. RESULTS: In the MPP+ treatment group, the neurites grew slowly and sparsely, dead cells were found in all regions. In the co-culture group, the neuritis grew densely, only a few cells were dead, the number of tyrosine hydroxylase(TH)-stained neurons increased and the structure of organellae was normal. CONCLUSION: MSCs may protect dopaminergic neurons against damage induced by MPP+. These results provide some data for cell transplantation therapy to Parkinsons disease.  相似文献   

Vasonatrin peptide attenuates injuries of dopaminergic neurons induced by 1-methyl-4-phenylpyridinium     

YU Jun  CHEN Bao-ying  ZHAO Ge  ZHANG Xue-ce  ZHAO Hai-kang 《园艺学报》2014,30(5):832-836

AIM：To investigate the neuroprotective effects of vasonatrin peptide (VNP) on the injury of dopaminergic neurons induced by 1-methyl-4-phenylpyridinium (MPP+). METHODS：Cultured dopaminergic neurons from the mouse ventral mesencephalon were exposed to MPP+, and the effects of VNP on the neurotoxicity of MPP+ were eva-luated by cell viability analysis and immunofluorescence staining. Various kinds of agonists and antagonists were used to clarify the mechanism underlying the effects of VNP. RESULTS：MPP+ caused injuries in the dopaminergic neurons. VNP significantly increased the viability, axon number and axon length of the dopaminergic neurons. The MPP+-induced depolymerization of β-tubulin Ⅲ was also attenuated by the treatment with VNP. In addition, VNP significantly increased the intracellular levels of cGMP. These effects of VNP were mimicked by 8-Br-cGMP (a cell-permeable analog of cGMP), whereas inhibited by HS-142-1 ［the antagonist of the particulate guanylyl cyclase-coupled natriuretic peptide receptors (NPR)］, or KT-5823 ［a cGMP-dependent protein kinase (PKG) inhibitor］. CONCLUSION：VNP attenuates the neurotoxicity of MPP+ via guanylyl cyclase-coupled NPR/cGMP/PKG pathway, indicating that VNP might be a new effective reagent in the treatment of neural degeneration of dopaminergic neurons in Parkinson disease.  相似文献   

Comparison of arylalkylamine N-acetyltransferase and melatonin receptor type 1B immunoreactivity between young adult and aged canine spinal cord     

Ji Hyeon Ahn  Joon Ha Park  In Hye Kim  Jae-Chul Lee  Bing Chun Yan  Min Sik Yong  Choong Hyun Lee  Jung Hoon Choi  Ki-Yeon Yoo  In Koo Hwang  Seung Myung Moon  Hyung-Cheul Shin  Moo-Ho Won 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):335-342

Melatonin affects diverse physiological functions through its receptor and plays an important role in the central nervous system. In the present study, we compared immunoreactivity patterns of arylalkylamine N-acetyltransferase (AANAT), an enzyme essential for melatonin synthesis, and melatonin receptor type 1B (MT2) in the spinal cord of young adult (2~3 years) and aged (10~12 years) beagle dogs using immunohistochemistry and Western blotting. AANAT-specific immunoreactivity was observed in the nuclei of spinal neurons, and was significantly increased in aged dog spinal neurons compared to young adult spinal neurons. MT2-specific immunoreactivity was found in the cytoplasm of spinal neurons, and was predominantly increased in the margin of the neuron cytoplasm in aged spinal cord compared to that in the young adult dogs. These increased levels of AANAT and MT2 immunoreactivity in aged spinal cord might be a feature of normal aging and associated with a feedback mechanism that compensates for decreased production of melatonin during aging.  相似文献   

北京鸭脑内蓝斑的解剖     

林大诚 《中国农业大学学报》1994,(1):97-99

在光镜下观察北京鸭脑的连续切片。并将 HRP 注入食管、胃、心脏和脊髓,逆向追踪迷走神经背核传出神经元在核内的定位。北京鸭迷走神经背核位于延髓呈纵向细胞柱,可明显地分为背侧部和腹侧部。腹侧部含淡染小细胞,多为梭形,细胞直径18～20 μm。背侧部内含中小型细胞,混合排列,直径20～35 μm;在闩前的后段还出现大细胞,直径35～40 μm。在二部的外侧有一小部为外侧部。本文提出按部位划分亚核的原则以利应用。共分为尾侧亚核、吻侧亚核、闩前背侧后核、闩前腹侧后核、闩前外侧后核、闩前背侧前核、闩前腹侧前核、闩前外侧前核共8个亚核。用 HRP 法追踪投射到食管各段、腺胃和肌胃薄肌与厚肌、心脏以及脊髓的神经元,肯定了北京鸭迷走神经背核投射至以上各外周器官的神经元在核内有一定的定位区域。  相似文献   

支配猪左心室侧壁的交感神经节后神经元的定位          下载免费PDF全文

雷治海  刘家因 《西北农林科技大学学报(自然科学版)》1988,(1):22-27

用HRP法研究了猪左心室外侧壁的交感神经节后神经元的分布,结果表明:标记细胞位于左侧颈中神经节、右侧椎神经节、双侧颈胸神经节及第二至第四胸交感神经节。标记细胞以颈胸节最多,占59.4％。左侧出现的标记细胞数明显多于右侧。标记细胞为多突起的星形运动神经元,以直径20～30μ为主,占67％。颈胸神经节内的标记细胞,大部分位于节的前腹侧部。颈前神经节内未发现标记细胞。  相似文献   

鸡胆囊的交感传入神经元定位研究   总被引：3，自引：0，他引：3  

剡海阔  刘英 《甘肃农业大学学报》2004,39(3):270-272

选用体重1．5～2．5kg的成年母鸡6只，将CT—HRP溶液注入胆囊壁，动物存活3～4d后，经左心室灌流固定，取胸、腰和荐段各脊神经节，制成50gm的连续冰冻切片，TMB法呈色反应，置明视野显微镜下观片统计。结果发现：支配鸡胆囊的交感传入神经元胞体位于T2～T7脊神经节，其峰值位于T6脊神经节。  相似文献