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Francisco Linhares Arruda Ferreira Gomes Fabíola Fernandes Heredia Priscilla Barbeta e Silva Olivardo Facó Francisco de Assis de Paiva Campos 《Scientia Horticulturae》2006
We present here a method for the regeneration of the prickly-pear (Opuntia ficus-indica (L.) Mill.) through somatic embryogenesis (SE). Direct SE was induced by cultivating shoot apices devoid of leaf primordia on semisolid Murashige and Skoog (MS) basal medium supplemented with 4-amino 3,5,6-trichloropicolinic acid (picloram) at 4 mg l−1. Somatic embryogenesis was influenced by the type, age, physiological and developmental stage of the explants, and also by light conditions, wounding, and the sucrose concentration in the induction medium. A histological analysis confirmed SE by revealing the presence of a closed vascular system in the developing embryos and the absence of a vascular connection between the somatic embryos and the explant. 相似文献
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Picloram,ABA和TDZ对香蕉体细胞胚胎发生的影响 总被引：5，自引：1，他引：5
研究Picloram、ABA和TDZ对贡蕉未成熟花序来源的胚性细胞悬浮系体细胞胚胎发生的影响。结果表明, 8.28 μmol/L Picloram替代2, 4-D可获得15.56%的胚性愈伤组织诱导率; 在体细胞胚诱导初期加入ABA则抑制体细胞胚的发生, 并造成其萌发时严重愈伤化; TDZ可有效地改善体胚萌发率和植株转换率, 其中0.2 μmol/L TDZ处理的体胚萌发率和植株转换率分别从对照的17.28%、16.49%提高到72.77%和67.05%。 相似文献
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