EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion     

Yun Wang  Ge Liu  Rui Liu  Maosheng Wei  Jinxiang Zhang  Chaomin Sun 《Marine drugs》2021,19(3)

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.  相似文献   

互补野油菜黄单胞菌野油菜致病型T113突变体胞外多糖产生的DNA片段的克隆     

韦珂  唐纪良  冯家勋 《广西农业生物科学》1999,18(1)

以从野油菜黄单胞菌野油菜致病型（Ｘａｎｔｈｏｍｏｎａｓｃａｍｐｅｓｔｒｉｓｐｖ．ｃａｍｐｅｓｔｒｉｓ）胞外多糖突变体Ｔ１１３中克隆的含转座子Ｔｎ５ｇｕｓＡ５及其两侧相邻序列的１２．３ｋｂＥｃｏＲＩＤＮＡ片段为探针,从野生型菌株８００４中克隆到与突变位点相对应的６．５ｋｂＥｃｏＲＩ片段,克隆于载体ｐＬＡＦＲ３上的该片段能反式互补突变株Ｔ１１３的胞外多糖产生,说明该片段上含有至少一个与胞外多糖产生有关的基因。  相似文献   

产胞外多糖的黑鲷肠道乳酸菌的分离鉴定及其生物学特性     

戴贤君  章蔚  刘明启  黄晓林 《浙江大学学报(农业与生命科学版)》2011,37(2)

通过常规鉴定和16S rDNA序列分析,从黑鲷(Sparus macrocephalus)肠道中分离鉴定出1株明串珠菌属(Leuconostoc)乳酸菌菌株;该分离株对酸、胆盐、蛋白酶具有良好耐受性,但对温度耐受能力较差;分离株培养上清液对哈维氏弧菌(Vibrio harveyi)、溶藻弧菌(Vibrio algnolyticus)、副溶血弧菌(Vibrio parahaemolyticus)的抑菌圈直径为1.12～1.22 cm,不同培养时间的抑菌效果差异无统计学意义(P＞0.05);分离株在培养24、32、40、48和56 h时的胞外多糖质量浓度为167.24～184.45 mg·L-1,不同取样时间的胞外多糖质量浓度差异无统计学意义(P＞0.05);灌服胞外多糖20 d后黑鲷血液的红细胞、白细胞数量和血红素浓度、淋巴细胞比率均显著升高(P＜0.05).综上,该分离株为产胞外多糖的明串珠属乳酸菌,可作为开发海水鱼用微生态制剂的候选菌株.  相似文献   

一株葡糖杆菌胞外多糖的体外抗氧化活性及其组成研究   总被引：2，自引：0，他引：2  

郭丹钊  黄为一 《南京农业大学学报》2005,28(2):50-54

葡糖杆菌属菌株JS1(Gluconobactersp.)所产胞外多糖(EPS)经乙醇沉淀、Sevage法脱蛋白、透析、冷冻干燥等方法分离和纯化后得到精制多糖。该多糖呈现明显的体外抗氧化活性,0.2g·L-1的添加量就可显著抑制饱和脂质的氧化,至第9天抑制率可达93.7%,效果优于柠檬酸对照处理(87.7%);对·OH有良好的清除能力,效果与维生素C对照相当,1.0g·L-1的添加量对·OH的清除率可达87.12%。通过柱层析法对精制多糖进一步分离得到P1、P2两个单一组分,并经聚丙烯酰胺凝胶电泳鉴定其纯度。通过气相色谱和核磁共振分析确定,P1由阿拉伯糖、木糖和半乳糖组成,三者的含量比为1.83∶0.36∶1.00,含α型和β型糖苷键,以β型为主;P2由鼠李糖和甘露糖组成,两者含量比为1.49∶1.00,仅含β型糖苷键。  相似文献   

嗜水气单胞菌双组分系统FlrBC的功能探究     

马倩倩  周洋  张永安 《华中农业大学学报》2022,41(3):221-228

为明确双组分系统FlrBC在嗜水气单胞菌极生鞭毛合成、生物被膜形成及致病机制中的作用,通过同源重组法,以嗜水气单胞菌ZYAH72为野生菌株构建双组分系统FlrBC缺失株ΔflrB和ΔflrC,比较不同菌株鞭毛合成及游动性、生物被膜形成、胞外多糖分泌、细胞黏附以及抗全血杀伤能力差异。结果显示:与野生株相比,ΔflrB和ΔflrC仍能形成极生鞭毛,细菌游动能力无显著差异;而结晶紫染色发现ΔflrB和ΔflrC相较于野生株的生物被膜形成能力分别下降了27.2%和22.3%;刚果红检测结果显示,与野生株相比,ΔflrB和ΔflrC的胞外多糖分泌分别下降了18.4%和14.2%;实时荧光定量PCR的结果显示,flrB和flrC的缺失不同程度抑制了鞭毛合成、生物被膜相关通路基因的表达;与草鱼肾细胞共孵育后,ΔflrB和ΔflrC的细胞黏附率与野生株相比分别下降了23.2%和18.2%;全血杀伤实验结果显示,ΔflrB和ΔflrC抗全血杀伤能力均显著减弱。以上结果表明,双组分系统FlrBC不是嗜水气单胞菌极生鞭毛形成所必需,但在细菌鞭毛形成组装和生物被膜形成中发挥调控作用,并且影响嗜水气单胞菌致病性...  相似文献   

C源和N源对周毛德克斯氏菌产生胞外多糖的影响     

林志勇  吴文礼  陈汉清 《福建农林大学学报(自然科学版)》2001,30(2):217-222

周毛德克斯氏菌产生胞外多糖的培养液需用净化自来水配制 ;该菌株产生多糖不要求高通气量 ,150 r· min-1下以 50 0 m L三角瓶装 10 0 - 150 m L培养液为宜 ;以玉米糖、麦芽汁、糖蜜、酒糟等为 C源替代甘露醇的结果表明 ,玉米糖的效果显著优于其他 C源 ,其发酵液多糖产量为 19.35g· L-1,C源利用率为 82 .4 % ;以Na NO3、NH4 Cl、( NH4 ) 2 SO4 等 3种无机 N和蛋白胨、鱼粉、玉米浆、黄豆粉等 4种有机 N作 N源的结果表明 ,玉米浆的作用最显著 ,能有效地提高多糖产量及发酵液的粘度  相似文献   

Use of Natural Antimicrobial Peptides and Bacterial Biopolymers for Cultured Pearl Production     

Christelle Simon-Colin  Yannick Gueguen  Evelyne Bachere  Achraf Kouzayha  Denis Saulnier  Nicolas Gayet  Jean Guezennec 《Marine drugs》2015,13(6):3732-3744

Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with two marine bacterial exopolymers as alternative treatment agents. In field studies, the combination treatment resulted in a significant reduction in graft failures vs. untreated controls. The combination of tachyplesin (73 mg/L) with two bacterial exopolysaccharides (0.5% w/w) acting as filming agents, reduces graft-associated bacterial contamination. The survival data were similar to that reported for antibiotic treatments. These data suggest that non-antibiotic treatments of pearl oysters may provide an effective means of improving oyster survival following grafting procedures.  相似文献   

ACC deaminase-producing bacteria mediated drought and salt tolerance in Capsicum annuum     

Ann Maxton  Poonam Singh  Sam A. Masih 《Journal of plant nutrition》2018,41(5):574-583

Influence of drought and salt stress on different morphological and physiological growth parameters in Capsicum annuum inoculated with our isolates was estimated during the present study. Bulkhorderia cepacia was reported to possess the maximal, whereas Citrobacter feurendii the least plant growth promoting efficacy under salt and drought stress. ACC Deaminase activity of purified B. cepacia, C. feurendii and Serratia marcescens was 12.8 ± 0.44, 12.3 ± 0.56 and 11.7 ± 0.53 μM αKB mg^?1 min^?1 respectively. Under drought stress, B. cepacia showed maximum tolerance as it produced 4.893 ± 0.06 mg/mg protein of exopolysaccharide followed by C. feurendii and S. marcescens that produced 4.23 ± 0.03 and 3.46 ± 0.05 mg/mg protein, respectively. Chlorophyll “a” concentration was recorded 5.7 gm L^?1 in B. cepacia inoculated plant (without stress) and was sustained till 2.9 gm L^?1 even under the highest tested drought period. Chlorophyll “a” concentration in the B. cepacia inoculated plant under the highest tested NaCl concentration was 3.2 gm L^?1. Thus, bacterial inoculation mitigates the effects of salinity by the proliferation of root system, increasing plant biomass proving to be potential bioinoculum for alleviating abiotic stress.  相似文献   

十字花科黑腐病菌中一个假定蛋白基因XC_3605与致病相关     

王凛  徐勇  韦载娲  阳丽艳  杨丽超  姜伯乐 《植物病理学报》2016,46(2):258-264

十字花科黑腐病菌(Xanthomonas campestris pv.campestris,Xcc)是全球范围内能引起十字花科植物黑腐病的重要植物病原细菌,也是研究植物病原细菌与植物互作机制的模式细菌。利用自杀质粒pK18mobsacB诱变十字花科黑腐病菌Xcc 8004的XC_3605基因,获得缺失突变体D3605。表型分析发现D3605胞外多糖合成能力、游动能力及致病力显著降低,积聚形成生物被膜的能力增强。用带有全长XC_3605基因序列的pLAFRJ对D3605进行功能互补,其胞外多糖产量、游动性、致病力和生物被膜均得到恢复。研究结果表明,XC_3605基因在十字花科黑腐病菌致病过程中发挥作用。  相似文献   

Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation,Biofilm Formation,and Breast Cancer     

Nesrine Gargouch  Fatma Elleuch  Ines Karkouch  Olfa Tabbene  Chantal Pichon  Christine Gardarin  Christophe Rihouey  Luc Picton  Slim Abdelkafi  Imen Fendri  Cline Laroche 《Marine drugs》2021,19(2)

Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs. However, lot of them remain to be discovered and tested. In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells. Results have shown that all EPS samples present some biological activity. For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain. For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration. Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%. Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.  相似文献