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A. Tullu L. Buchwaldt M. Lulsdorf S. Banniza B. Barlow A. E. Slinkard A. Sarker B. Tar’an T. Warkentin A. Vandenberg 《Genetic Resources and Crop Evolution》2006,53(1):111-119
Lentil anthracnose (Colletotrichum truncatum (Schwein.) Andrus et W.D. Moore is a potential threat in many lentil (Lens culinaris Medik.) production regions of North America. In the lentil germplasm maintained in Germany and North America, 16 lines were
reported to have resistance to race Ct1, but none has resistance reported to race Ct0. The objective of this study was to
examine accessions of wild Lens species for their resistance to races Ct1 and Ct0 of lentil anthracnose. Five hundred and seventy-four wild accessions of
six species and control lines were screened in two replications under both field and greenhouse conditions using a 1–9 scoring
scale (1, highly resistant; 2–3, resistant; 4–5, moderately resistant; 6–7, susceptible; and 8–9, highly susceptible). Indianhead
and PI 320937 were resistant while Eston and Pardina were susceptible to race Ct1 as expected. However, none of the check
lines were resistant to race Ct0. Among the six Lens wild species tested, accessions of Lens ervoides (Brign.) Grande had the highest level of resistance, 3–5 to race Ct1 and Ct0 followed by L. lamottei Czefr. in the field and greenhouse. Lens orientalis (Boiss.), L. odemensis L., L. nigricans (M. Bieb.) Godron and L. tomentosus L. were highly susceptible, 8–9 to race Ct0 in the greenhouse. The highest frequency of resistance, especially in L. ervoides (Brign.) Grande, was found in accessions originating from Syria and Turkey. The usefulness of these L. ervoides (Brign.) Grande accessions as sources of resistance to the more virulent race of anthracnose in a lentil breeding program
is discussed. 相似文献
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Complete removal of roots from soil samples is a prerequisite for most of the chemical and biological analyses. A simple electrostatic method of separating roots from sieved, largely mineral soil substrates was optimized and examined by the addition of 14C labeled fine roots to sandy, silt loamy and clay loamy samples. Depending on soil texture, between 40% and 50% of fine roots can be removed from 100 g of sieved soil in less than 10 minutes. The root‐free soil substrate and the extracted roots can be used for analyzes or experiments immediately after the separation. The proportion of the mineral particles remaining in the root fraction depends on duration of separation, distance between the charged plate and the sample, and soil texture. The proportion of separated mineral particles is about 90%—95% (w/w) in sandy and 70%—85% in silt loamy and clay loamy substrates. The electrostatic method of root separation may take place before the analysis of Ct and Nt contents, and is suitable for soil samples preparation for incubation experiments. 相似文献
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采用测定辐照产品箱中吸收剂量不均匀度的方法对5种常用辐照产品的3种辐照加工工艺质量进行了研究。结果如下:1)上下对调并前后翻转的双面辐照工艺,其产品箱中的吸收剂量不均匀度U范围为1.36-1.67,在辐照加工质量控制标准(U<2)以内,可用于生产。2)单面辐照方式其剂量不均匀度为2.67,超过了质量控制标准,不宜采用。3)后排单面辐照方式其剂量不均匀度(2种产品)分别为3.25和3.69,远大于U<2的标准,不宜作为主要辐照方式,但可以作为辅助辐照方式以提高^60Co源的利用率。 相似文献
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【目的】研究亚洲韧皮杆菌(CLas)在纽荷尔脐橙病树的分布情况,为柑橘黄龙病(HLB)的防控提供参考依据。【方法】以1~3个枝条表现斑驳黄化叶片症状的纽荷尔脐橙病树为研究对象,对主干发出的5个主枝(A、B、C为斑驳黄化枝条,D、E为无症状枝条)的叶片分别取样,运用PCR和实时荧光定量PCR(qPCR)分别检测,用2-ΔΔCt法分析树体各部分黄龙病菌含量。对于果树上表现典型HLB病果但叶片无症状的结果枝,分别检测典型病果和相应叶片的感病情况。【结果】PCR对A、B、C、D、E的阳性检出率分别为88.57%、61.11%、54.17%、6.67%和0,qPCR对A、B、C的阳性检出率均为100.00%,对D、E的阳性检出率分别为40.00%和13.33%;2-ΔΔCt法分析发现,斑驳黄化叶片中病原菌含量更高,病果的阳性检出率高于相应的无症状叶片样品。【结论】CLas在表现典型斑驳黄化症状的叶片和果实中的含量及阳性检出率均较高,病原菌在病树中分布不均匀。在进行HLB检测时,应选取典型或疑似症状样品,并运用qPCR进行检测,一旦发现树体局部发病,应当整株销毁。 相似文献
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实时荧光定量PCR在植物病害中的应用 总被引:5,自引:1,他引:4
实时荧光定量PCR技术作为一项新兴技术,越来越受到人们的重视。它以快速、准确定量、便捷等优点广泛应用于科学研究各个领域,近年来, 实时荧光定量PCR技术在植物病害研究上不断深入,大大提高了植物病害的检测效率和监测防治水平。本文重点就对实时荧光定量PCR技术在由真菌、细菌、病毒、线虫引发的植物病害的检测与应用;并结合了最近几年来应用于检测和鉴定植物病原病害的实时荧光定量PCR过程中引物与探针的设计、反应过程、结果评价情况做综合论述。 相似文献
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在室内半静水的试验条件下,应用实时荧光定量PCR技术研究了不同质量浓度(0μg·L-1、5μg·L-1、10μg·L-1、50μg·L-1和100μg·L-1)的0。柴油水溶液(WSF)胁迫15d和清洁海水恢复7d中翡翠贻贝(Pernaviridis)外套膜与内脏团组织中CYP4基因的相对表达水平变化。2-△△Ct法分析结果表明,CYPd基因在翡翠贻贝外套膜和内脏团中均有表达,WSF胁迫对其表达水平有明显的诱导作用,且表达水平具有组织差异性;内脏团表达水平明显高于外套膜,两组织CYP4基因相对表达水平随着WSF胁迫时间的延长,整体呈现出先诱导后抑制再诱导的波动变化趋势,其中50μg·L-1浓度组表现最为显著(P〈0.01)。WSF胁迫解除后外套膜与内脏团CYP4基因相对表达水平迅速下降,部分浓度组逐渐恢复到正常水平。 相似文献
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