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Michaela Schiller Mette Lübeck Thomas Sundelin Luis Fernando Campos Meléndez Solveig Danielsen Dan Funck Jensen Kenneth Madriz Ordeñana 《European journal of plant pathology / European Foundation for Plant Pathology》2006,116(2):107-118
Strawberry, Fragaria × ananassa, and leatherleaf fern, Rumohra adiantiformis, are two important crops in Costa Rica. One of the most severe diseases affecting these crops is anthracnose, caused by members of the fungal genus, Colletotrichum (teleomorph; Glomerella). Eighty single-spore isolates from strawberry and leatherleaf fern were identified as Colletotrichum acutatum by species-specific PCR, and were further characterised by Universally Primed PCR (UP-PCR) fingerprinting analysis, and sequence analysis of the ribosomal internal transcribed spacer (ITS) region. Morphological differences, genotypic variation revealed by UP-PCR fingerprinting analysis, and a single sequence polymorphism within the ITS2 region were found between the isolates from strawberry and leatherleaf fern, respectively. The UPGMA cluster analysis of the fingerprints clearly separated the isolates derived from strawberry and leatherleaf fern into two different clusters. Pathogenicity assays on detached strawberry fruits confirmed the apparent difference between the two groups of isolates. It is therefore suggested that the pathogens responsible for strawberry anthracnose fruit rot and leatherleaf fern anthracnose in Costa Rica, belong to two distinct subpopulations of C. acutatum. 相似文献
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采用Universally Primed PCR(UP-PCR)技术对分离自辽宁省不同地区的西瓜、甜瓜、黄瓜枯萎病株的21株尖孢镰孢菌菌株进行遗传多样性分析。结果表明:6条通用引物共扩增出61条带,其中多态性条带58条,占总条带数的95.1%。通过聚类分析,在相似系数0.746处将21株菌株分为6个类群:FOG1类群包含6株甜瓜尖孢镰孢菌菌株,FOG2包含6株西瓜专化型尖孢镰孢菌菌株,FOG3包含6株黄瓜专化型尖孢镰孢菌菌株,FOG4包含1株西瓜专化型尖孢镰孢菌菌株,FOG5包含1株甜瓜尖孢镰孢菌菌株,FOG6包含1株黄瓜专化型尖孢镰孢菌菌株。 相似文献
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保护地土壤生防木霉菌种群多样性研究 总被引：2，自引：0，他引：2
采用传统的形态学特征和分子方法(ITS、TEF序列和UP-PCR)研究了蔬菜保护地土壤中木霉菌种群多样性及其影响因素.结果表明:提交genbank 11个木霉种的序列,分别为长枝木霉(Trichoderma longibrachiatum)、拟康氏木霉(Trichoderma pseudokoningii)、黄绿木霉(Trichoderma aureoviride)、棘孢木霉(Trichoderma asperellum)、深绿木霉(Trichoderma atroviride)、哈茨木霉(Trichoderma harzianum)、非钩木霉(Trichoderma inhamatum)、微孢木霉(Trichoderma minutisporum)、长孢木霉(Trichoderma longipile)和粘绿木霉(Trichoderma virens);木霉菌24个菌株经UP-PCR扩增,引物AS4、AS19、L45扩增出一条500 bp大小的木霉菌种的特征性谱带,其他谱带则为多态性谱带,多态性达93.5％;营养条件、杀菌剂及土壤因素对不同种木霉菌的影响不同,得到2株适应性较强的木霉菌株,有望成为生防菌株. 相似文献
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西南地区玉米纹枯病病菌融合群鉴定和UP-PCR分析 总被引：2，自引：1，他引：1
为明确西南地区玉米纹枯病病菌组成结构,从西南地区(四川、贵州、云南和重庆)玉米纹枯病样上分离获得285个疑似丝核菌菌株,根据培养性状、菌丝形态和细胞核染色对其进行鉴定,通过载玻片配对培养法对鉴定出的菌株进行菌丝融合群鉴定,并采用UP-PCR方法从各融合群中选取代表性菌株进行遗传变异分析。结果显示,供试疑似菌株中共鉴定出253个丝核菌,分别为224株立枯丝核菌Rhizoctonia solani和29株玉蜀黍丝核菌R.zeae。立枯丝核菌包括5个融合群,其中AG1-ⅠA共201株,出现频率为79.4%;AG1-ⅠB共2株,出现频率为0.8%;AG2-2ⅢB共3株,出现频率为1.2%;AG4-HGⅠ共10株,出现频率为3.9%;AG-5共8株,出现频率为3.2%。29株玉蜀黍丝核菌融合群均为WAG-Z,出现频率为11.5%。遗传变异分析中,当相似系数为0.78时,按照出现频率从立枯丝核菌和玉蜀黍丝核菌选取的不同融合群的20株菌株被划分为6个类群,与菌丝融合分析结果完全吻合,其中AG2-2ⅢB融合群首次从西南地区玉米上分离到。 相似文献
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应用UP-PCR进行玉米丝黑穗病菌遗传多样性研究 总被引：5，自引：0，他引：5
本研究探讨了UP PCR技术在玉米丝黑穗病菌遗传多样性分析中的利用可行性。在13个通用引物中,筛选出9个扩增多态性好且稳定的通用引物,共扩增出113条DNA条带,大小分布于250～2 000 bp之间,其中多态性条带为95条,为总条带数的84.07%。遗传距离为0.76处时,所有丝黑穗病菌菌株被聚为6个组。利用UP-PCR技术,可以充分展现玉米丝黑穗病菌菌株间的亲缘关系及差异性,可用于玉米丝黑穗病菌遗传多样性研究,为有效地开展玉米丝黑穗病菌的遗传进化甚至探讨病菌致病性的生理分化提供了一个新的技术方法。 相似文献
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Sari Paavanen-Huhtala Hanna Avikainen Tapani Yli-Mattila 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(2):187-198
The randomly amplified polymorphic DNA (RAPD) technique was used to develop strain-specific primers for Gliocladium catenulatum strain J1446, which is promising in biological control. One of the primer pairs developed proved to be strain-specific; strain J1446 was differentiated from 16 G. catenulatum strains and six other strains of two Gliocladium species, as well as from Trichoderma virens, and isolates of Nectria spp. and Fusarium spp. Specific primers were also tested with DNA isolated from cucumber leaves, treated or untreated with a solution made from Gliocladium powder. The expected amplification product was produced only from treated leaves. DNA isolated from Gliocladium-treated potato tubers and fungi grown in peat was also used in amplification reactions. Strain-specific primers detected strain J1446 when the amount of DNA was 5pg or more. Some variation between the Gliocladium strains was found by the random amplified microsatellites method (RAMS) and the universally primed polymerase chain reaction method (UP-PCR), but no clear fragments specific to strain J1446 were produced. Cross-blot hybridisation of UP-PCR products differentiated strain J1446 from T. virens, but not from the Gliocladium isolates. The 28S rDNA sequences and -tubulin sequences were identical or very similar in all Gliocladium strains. Thus, it is possible that the Gliocladium strains of the present study are conspecific, which means that a revision in the taxonomy of Gliocladium species may be necessary. 相似文献
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现代分子生物学技术在木霉鉴定及多样性分析中的应用 总被引：3，自引：0，他引：3
木霉是一类重要的生防真菌,具有很大的农业应用潜力。对分离培养的木霉准确鉴定,对其在农业生产中的应用至关重要。近年来,分子生物学技术在木霉鉴定方面得到了广泛应用,本文综述了rDNA-ITS序列分析、通用引物PCR(UP-PCR)、随机扩增多态性DNA(RAPD)、扩增片段长度多态性(AFLP)等分子技术在木霉鉴定与多样性分析中的应用。 相似文献
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Mette Lübeck 《浙江大学学报(农业与生命科学版)》2004,30(4):463-463
Universally Primed PCR (UP-PCR) is a PCR fingerprinting method that has demonstrated its applicability in different aspects of mycology. These applications constitute analysis of genome structures, identification of species, analysis of population and species diversity, revealing of genetic relatedness at infra-and inter-species level, and identification of UP-PCR markers at different taxonomic levels (strain, group and/or species) . A further development of the UP-PCR technique is an … 相似文献
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