首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   88篇
  免费   26篇
  国内免费   2篇
林业   1篇
农学   1篇
  1篇
综合类   5篇
农作物   1篇
水产渔业   10篇
畜牧兽医   66篇
植物保护   31篇
  2023年   1篇
  2022年   12篇
  2021年   6篇
  2020年   10篇
  2019年   4篇
  2018年   11篇
  2017年   6篇
  2016年   5篇
  2015年   10篇
  2014年   14篇
  2013年   8篇
  2012年   5篇
  2011年   2篇
  2010年   5篇
  2009年   4篇
  2008年   4篇
  2007年   3篇
  2006年   2篇
  2004年   1篇
  2001年   1篇
  2000年   1篇
  1998年   1篇
排序方式: 共有116条查询结果,搜索用时 31 毫秒
1.
疫病成为制约我国畜牧业发展的主要因素,要较好控制疫病必须推行生物安全措施。本文从生物安全概念、推行生物安全必要性、生物安全范围、生物安全难点及养鸡场生物安全措施进行了论述。  相似文献   
2.
3.
BackgroundDiarrheagenic E. coli (DEC) strains are a major cause of diarrheal diseases in both developed and developing countries. Healthy asymptomatic animals may be reservoirs of zoonotic DEC, which may enter the food chain via the weak points in hygiene practices.AimWe investigated the prevalence of DEC along the pig production continuum from farm-to-fork.MethodsA total of 417 samples were collected from specific points along the pig production system, that is, farm, transport, abattoir and food. E. coli was isolated and enumerated using Colilert. Ten isolates from each Quanti-tray were selected randomly and phenotypically identified using eosin methylene blue agar selective media. Real-time polymerase chain reaction (PCR) was used to confirm the species and to classify them into the various diarrheagenic pathotypes. Antimicrobial susceptibility was determined against a panel of 20 antibiotics using the Kirby-Bauer disk diffusion method and EUCAST guideline.ResultsThe final sample size consisted of 1044 isolates, of which 45.40% (474/1044) were DEC and 73% (762/1044) were multidrug-resistant. Enteroinvasive E. coli (EIEC) was the most predominant DEC at all the sampling sites.ConclusionThe presence of DEC in food animal production environments and food of animal origin could serve as reservoirs for transmitting these bacteria to humans, especially in occupationally exposed workers and via food. Adherence to good hygienic practices along the pig production continuum is essential for mitigating the risk of transmission and infection, and ensuring food safety.  相似文献   
4.
In recent years several interspecific hybrids have been reported in the plant pathogenic oomycete genus Phytophthora. Due to the large genotypic and phenotypic changes, these hybrids might have broader or more limited host ranges compared with their parental species. It is crucial to understand the host range of Phytophthora hybrids to minimize the economic losses caused by their infection. The potential host range of four hybrids belonging to Clade 8a of the Phytophthora phylogenetic tree was investigated in this study. Thirty species of herbaceous plants as well as eight species of woody plants were inoculated and monitored for any symptom of infection. In addition, the detached twigs of 32 tree species, fruits of six plant species, tubers of potato, and roots of carrot and sugar beet were investigated for susceptibility to these hybrids. Almost all hybrids caused severe rot on all tested fruits, tubers, and roots, although different isolates showed different pathogenicity on detached tree twigs. All hybrids tested had a different host range compared with their parental species: they were able to infect plants outside the host range of their parents, infect hosts of both parental species, although these parents did not have overlapping hosts, or, in some cases, they were not able to infect hosts infected by the parents.  相似文献   
5.
土壤病原微生物检测技术研究进展   总被引:1,自引:0,他引:1  
病原微生物如细菌、病毒、真菌等广泛存在于土壤环境中,其严重威胁着全球公共卫生安全。发展病原微生物快速检测技术,对于减少病原菌扩散传播、防控传染性疾病、维护生物安全具有重要意义。本文对病原微生物检测技术的最新研究进展进行了综述,包括显色培养技术、基质辅助激光解吸电离飞行时间质谱、分子生物学技术、拉曼光谱技术、流式细胞技术和生物传感器等,并对其原理、应用及优缺点进行了全面比较。最后,对病原微生物检测技术的未来发展提出展望,旨在促进土壤环境病原微生物的快速检测和生物风险防控。  相似文献   
6.
Closed recirculating aquaculture systems (RAS) offer advantages over traditional culture methods including enhanced biosecurity, the possibility of indoor, inland culture of marine species year‐round and potential marketing opportunities for fresh, never‐frozen seafood. Questions still remain regarding what type of aquaculture system may be best suited for the closed‐system culture of marine shrimp. In this study, shrimp (Litopenaeus vannamei) were grown in clear‐water RAS and in biofloc‐based systems. Comparisons were made between the system types with respect to water quality, shrimp production and stable isotope dynamics used to determine the biofloc contribution to shrimp nutrition. Ammonia and nitrite concentrations were higher, and shrimp survival was lower in the biofloc systems. Although stable isotope levels indicated that biofloc material may have contributed 28% of the carbon and 59% of the nitrogen in shrimp tissues, this did not correspond with improved shrimp production. Overall, the water column microbial communities in biofloc systems may be more difficult to manage than clear‐water RAS which have external filters to control water quality. Biofloc does seem to offer some nutritional contributions, but exactly how to take advantage of that and ensure improved production remains unclear.  相似文献   
7.
Citrus black spot was recorded as present in New Zealand in international databases on the basis of one isolate (ICMP 8336) identified by morphological features as Guignardia citricarpa . This isolate was from a soft rot, not a typical symptom of citrus black spot, on a Seminole tangerine fruit grown in the northern region of New Zealand. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1, 5·8S and ITS2) showed that this isolate was 99% identical to the ITS region of G. mangiferae , a closely related saprotroph. Despite climatic conditions being suitable for this disease, citrus black spot symptoms have never been seen on citrus fruits grown in New Zealand. Thus the absence of symptoms on citrus in New Zealand is probably because G. citricarpa is not present. On the basis of these results, the record of citrus black spot occurring in New Zealand should be re-examined.  相似文献   
8.
Poultry transportation coops are rarely washed and have been demonstrated to be a point of cross-contamination of broiler carcasses. Foaming disinfectants and cleaners, commonly used within processing plants, may be used to clean and disinfect poultry transportation coops. In this study, homogenized fecal material was evenly applied to the floors of precleaned broiler transportation coops and allowed to dry. Treatments consisted of a water rinse, a foam additive alone, foaming cleaner, and a disinfectant plus a foam additive. All foaming treatments were applied using a compressed air foam system (2,271 L/min; 600 gal/min), similar to what firefighters would use. A high-pressure water rinse (HPWR) was added prior to or following the treatments to determine whether rinsing prior to product application or rinsing after product application would improve efficacy. Based on our data, a compressed air foam system may be used in combination with a commercially available disinfectant or foam cleaner to reduce aerobic bacteria on the surfaces of commercial broiler transportation coops. Furthermore, the addition of a HPWR did not further reduce the level of aerobic bacteria on broiler transportation coop floors.  相似文献   
9.
10.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号