Correlation between hydrolytic enzyme activities measured in bean seedlings afterTrichoderma koningii treatment combined with pregermination and the protective effect againstPythium splendens     

Alba Marina Cotes  Philippe Lepoivre  Jean Semal 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(5):497-506

An experimental protocol consisting in the colonisation of pregerminated bean seeds dressed withTrichoderma sp. was used in order to study the mechanisms correlated with the protective effect againstPythium splendens. Seed dressed with TH-11 (T. koningii) for 24 h presented a higher protective effect and a higher level of seed colonisation as compared to those dressed with TH-13 (T. longibranchiatum). The levels of seed coat colonisation by TH-11 and TH-13 was shown to be correlated with the carboxymethylcellulase activity, as measured in the seed coats retreived from germinating dressed bean seeds. The seed coat colonisation was also associated with an increased activities of endo-1,3--glucanase and endochitinase measured in seed extracts, and an inhibitory effect of seed extracts onPythium sporangia germination. Pretreatment of TH-13-dressed seeds with a commercial cellulase improved all parameters mentioned above, thus suggesting a role of cellulase activity in the colonisation process and the linked protective effect. The possible role of hydrolytic enzymes in the protective effects is discussed.  相似文献   

温室集雨与集雨水高效利用研究   总被引：5，自引：0，他引：5  

魏兴琥  谢忠奎  李小雁  王亚军  张志山 《干旱地区农业研究》2003,21(1):11-16

在半干旱偏旱区设置了清除土表杂草、红沙覆盖、废膜覆盖和日光温室棚面4种不同的集流面处理,结合日光温室秋冬茬黄瓜、番茄不同灌水量试验,通过水的转化效率探讨不同集流面的使用价值和提高水转化效益的适宜灌水量.结果表明:温室棚面的年产流率最高为85.4%,其次为废膜集流面45.4%,清除土表杂草集流面的年产流率仅有8.2%.从单方水集流费用看,清除土表杂草>红沙集流面>废膜集流面>日光温室棚面,平均集流费用为2.18元/m3,单座温室棚面(450 m2)集流面加上温室间隔区废膜集雨(600 m2),集雨水总量可达到197 m3,能够满足温室一茬黄瓜和一茬番茄的需水量.  相似文献   

毒死蜱和甲氰菊酯对家蚕毒性与安全评价研究   总被引：24，自引：3，他引：24  

吴声敢  王强  赵学平  吴长兴  陈丽萍 《农药科学与管理》2003,24(9):11-14

通过食下毒叶法和接触法测定了毒死蜱和甲氰菊酯对家蚕的毒性。结果表明:食下毒叶法对2龄起蚕的LC50(48h)分别为:毒死蜱2.3322mg/kg桑叶和甲氰菊酯0.1522mg/kg桑叶,其浸渍桑叶的药液浓度分别为4.6622mg/L和0.3044mg/L。接触法中毒死蜱和甲氰菊酯对2龄起蚕的LC50值(24h)分别为:6.32μg/cm2和0.92μg/cm2。毒死蜱和甲氰菊酯的毒性比都<1,对家蚕的毒性为高毒。  相似文献   

拟垫跗螋生物学特性的研究     

王宗典  帅先福  龚秧初  王大智  王浩毅  周文豹 《中国生物防治》1994,(2)

拟垫跗螋是为害造纸原料荻和芦的害虫（蚜虫、蓟马、螨等）的重要捕食性天敌。在湖南洞庭湖区一年发生２代，以成虫和若虫在残杆、渣叶和土缝中越冬。成虫寿命第１代约４个月，第２代连越冬达１０个月。雌雄性比约５４。雌雄均可多次交尾，有的雌虫产卵后又可交尾。每头雌虫的产卵量平均３９．３粒。卵期平均６．５天。若虫期第１代近４个月，第２代连越冬约９个月。大田消长主要受地势高低、洪水、农药、食物等因素影响。并对保护利用跗螋控制害虫的措施进行了讨论  相似文献   

Sequences of the 3′ Halves of the Genomes of Barley Yellow Dwarf Virus-PAV cpA Isolates that Vary in Symptom Severity     

Jamila Mastari  Hervé Lapierre 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(8):801-811

Barley yellow dwarf virus (BYDV)-PAV isolates from USA have been separated into two distinct clusters (Chay et al. (1996) Virology 219: 57–65; Chay et al. (1996) Phytopathology 86: 370–377). Following this finding we have shown that BYDV-PAV is divided into two groups cpA and cpB based on their coat protein gene sequence, and distinct host preferences (Mastari et al. (1998) Phytopathology 88: 818–821). We have sequenced the complete 3 half of the genomes of two lethal and two mild cpA isolates and compared them with those of several known PAV cpA isolates to assess variability and locate potential determinants of severity. Open reading frames (ORFs) 3, 4, 5, 6 and the 3 untranslated regions had different percent homologies between isolates: ORF5 (92–97%), ORF3 (88–98%) 3-translational enhancer (87–100%) ORF4 (85–99%), 3 untranslated region (72–97%) and ORF6 (61–99%). In contrast to the mild isolates, the field-lethal isolates (FHv1 and FHv2) fell into the same cluster, regardless of the genomic region analysed. The isolates FHv1 and FHv2 differed from mild isolates by eight amino acid substitutions in ORFs 3 and 4, and insertions in ORF5. Four amino acid substitutions in the 17-kDa protein encoded by ORF4 caused a change in local net charge in the field-lethal isolates. Two insertions of four amino acids were identified in the C-terminal half of ORF5 of the field-lethal isolates, but were not present systematically in all lethal isolates analysed. The potential relationships of these differences in predicted amino acid sequences to disease severity are discussed.  相似文献   

Effects of herbicidal carbamates on mitochondria and chloroplasts     

D. Macherel  P. Ravanel  M. Tissut 《Pesticide biochemistry and physiology》1982,18(3):280-288

At concentrations near 2 × 10^?4M, barban, chlorpropham, and phenmedipham are inhibitors of the electron transfer in potato and mung bean mitochondria. The inhibition seems to be localized in the flavoprotein region. It affects preferentially the exogenous NADH dehydrogenation, in potato mitochondria (I₅₀, 10^?4M). Succinate dehydrogenation is less inhibited. At noninhibiting concentrations, the studied carbamates cannot uncouple the oxidative phosphorylations. Photosynthesis is completely inhibited by 2.10^?7M phenmedipham, 5 × 10^?5M barban, and 2 × 10^?4M chlorpropham. The inhibition takes place at the PS II level. Moreover, barban and chlorpropham are uncouplers of the photophosphorylations for concentrations between 5 × 10^?5 and 5 × 10^?4M. The effects observed on mitochondrial respiration can also be found on respiration of Acer cultured cells. The effects on isolated chloroplast photosynthesis are also observed for slightly higher concentrations on cultured Chlorella and on pea and oat leaf fragments.  相似文献   

贮藏期间板栗呼吸特性研究     

王雁萍  戴桂馥  吴健  李宗伟  秦广雍  苏明杰  武广  胡国驹 《果树学报》2000,17(4):282-285

对板栗贮藏期间呼吸速率及影响因素的研究发现：０－５℃贮藏能显著抑制板栗呼吸，能使板栗缓慢呼吸的稳定期延长至次年 ３月，但随贮期延长板栗组织愈加不耐受低 Ｏ２高 ＣＯ２环境；降低起始Ｏ２或提高起始 ＣＯ２浓度不能降低板栗的呼吸速率。  相似文献   

Synthesis of dihydroxyphenacyl glycosides for biological and medicinal study: β-oxoacteoside from Paulownia tomentosa     

Hidenori Tozuka  Michikazu Ota  Hisayoshi Kofujita  Kouetsu Takahashi 《Journal of Wood Science》2005,51(1):48-59

The protected structure of -oxoacteoside (tomentoside A), 2-oxo-2-(3,4-dihydroxyphenyl)ethyl 3-O-(2,3,4-tri-O-acetyl--l-rhamnopyranosyl)-4-O-caffeoyl--d-glucopyranoside 14 was synthesized in 14% overall yield in 11 steps, starting from d-glucose for biological and medicinal studies of phenylpropanoid glycosides. The first step was the preparation of a 3-O-rhamnopyranosyl disaccharide sugar core 2 from a suitably protected rhamnosyl trichloroacetimidate 10 and glucose derivative (diacetone-d-glucose 1) in 71% yield. To the glucose moiety of this sugar core, several protection/deprotection procedures were performed sequentially to obtain a fully acetylated sugar core 7 with a 4-OH group on the glucose moiety, in 57% yield in five steps. Thereafter, to the 4-OH group of the glucose moiety, selective 4-O-caffeoylation was achieved by proton-transfer esterification with 3,4-di-O-allylcaffeic acid 16 to give the caffeoyl disaccharide 11 in 97% yield. Then, it was converted to trichloroacetimidate 13 for a glycosylation donor in 90% in two steps. Finally, anomeric glycosylation was conducted with 2-oxo-2-(3,4-di-allyloxyphenyl)ethyl alcohol 19 with catalytic amounts of BF₃·Et₂O to give 2-oxo-2-(3,4-di-allyloxyphenyl)ethyl 2,6-di-O-acetyl-3-O-(2,3,4-tri-O-acetyl--l-rhamnopyranosyl)-4-O-(3,4-di-allyloxycaffeoyl)--d-glucopyranoside 14 in 60% yield. Deprotected intermediates of compounds 2, 11, 14, and 19 which were obtained in high yield would be useful for biological and medicinal studies of phenylpropanoid glycosides.Part of this study was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April, 2002  相似文献   

Salt distribution in response to optical stratification porosity and relative windspeed in a coastal forest in Niigata, Japan   总被引：3，自引：0，他引：3  

J.J. Zhu  Y. Gonda  T. Matsuzaki  M. Yamamoto 《Agroforestry Systems》2002,56(1):73-85

Wind velocity, vertical stand structure and seasalt distribution were measured at various heights inside a plantation of coastal pine forest, with thinning and unthinning, to assess whether any relationships exist between the wind profile, stand structure and seasalt distribution in the coastal pine forest. The vertical stand structure, i.e., optical stratification porosity (OSP), which is defined as vertical distribution of the proportion of sky hemisphere not obscured by tree elements inside a forest stand, was determined for each height by computer analysis of digital images taken with a hemispherical lens. The distribution of OSP in the coastal forest follows the Lambert-Beer's law with absorbency coefficient (). The relative windspeed within canopy can be described using the exponential form with the attenuation coefficient (). The sea-salt was collected using salt gauze (a surgical dressing of loosely woven cotton) both inside and outside the coastal forest, and the distribution of sea-salt within canopy was also in accordance with an exponential function. The relationships among windspeed, OSP and sea-salt indicate that the distributions of both sea-salt and windspeed within canopy were very closely correlated with the distribution of OSP. A linear relationship between OSP and sea-salt has been found. Additionally, linear regression between coefficients and has been obtained as well. Based on these relationships between OSP, wind and sea-salt in the coastal forest, the sea-salt distribution and wind profile within the canopy of the coastal forest can be predicted according to OSP. These results may therefore be useful in analyzing the effects of sea-salt on vegetation and evaluating the filter functions of coastal forests.This revised version was published online in November 2005 with corrections to the Cover Date.  相似文献   

Strategic Planning of Sustainable Development on the Estate Level – Contributions from Forest Growth and Yield Science     

Hans Pretzsch 《Forstwissenschaftliches Centralblatt》2003,122(4):231-249

Zusammenfassung:   Die Nachhaltigkeit richtete sich in der Gründerzeit der planmäßigen Forstwirtschaft auf die Holzproduktion (v. Carlowitz 1713, Cotta 1828, Hartig 1804, Hundeshagen 1826). In der Folgezeit wurde ein zunehmend breiteres Spektrum von Leistungen des Waldes in die Planung mit einbezogen (v. Hagen, 1867; Dieterich, 1957). Das heutige Verständnis von multifunktionaler Forstwirtschaft spiegelt sich in den sechs gesamteuropäischen Kriterien und Indikatoren für nachhaltige Forstwirtschaft wider (McPfe, 2000). Vorliegender Aufsatz umreißt, wie diese gesamteuropäischen Kriterien und Indikatoren für den Nachweis und die strategische Planung von Nachhaltigkeit auf Forstbetriebsebene eingesetzt werden können und was die Waldwachstumsforschung dazu beitragen kann. An einer Reihe von Beispielen wird ausgeführt, dass die Forstwirtschaft über eine solide Datenbasis für die Ableitung von Nachhaltigkeitskriterien und Indikatoren verfügt. Diese kann für das Monitoring der Waldentwicklung genutzt werden. Gestalterische Kraft entwickeln die Kriterien und Indikatoren aber erst, wenn sie auch in die strategische Planung einfließen. Für die strategische Planung stehen der Forstwirtschaft Wuchsmodelle, Betriebssimulatoren und Entscheidungsstützungssysteme zur Verfügung. Sofern diese die genannten Kriterien und Indikatoren berücksichtigen, ermöglichen sie Szenariorechungen auf Betriebsebene und eine umfassende Bewertung von Handlungsalternativen. Konzepte und Werkzeuge für den Übergang zu einer multifunktionalen strategischen Nachhaltsplanung sind weit entwickelt (v. Gadow 2003, Hanewinkel 2001, Pretzsch et al. 1998, Spellmann et al. 2001); ihre Einführung in die Forstplanung könnte einen beträchtlichen Innovationsstau beseitigen.  相似文献