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1.
为降低蔬菜采后体内多环芳烃(PAHs)对人体的危害风险,以豇豆为材料,利用不同浓度洗洁精、食盐、米酒、米醋、植物油及清水分别对豇豆豆荚进行浸洗,通过高效液相色谱-质谱联用法检测豇豆采后体内PAHs的含量,并筛选出豇豆体内PAHs的最佳净化方法。结果表明,米酒和米醋处理对豇豆体内PAHs的去除效果基本一致,其中米酒处理组的∑PAHs(美国环保局公布的优先监测的16种多环芳烃的含量总和)降低68.92%,米醋处理组的∑PAHs降低73.88%,且对萘、二苯并(a,h)蒽和茚并(1,2,3-c,d)芘等均有明显的去除效果;清水浸洗可有效降低豇豆体内的茚并(1,2,3-c,d)芘含量,但会使菲的含量增加;食盐处理使∑PAHs增加了77.15%,主要表现在增加了2、3环PAHs在豇豆体内的积累;植物油处理可降低个别PAHs单体含量,但会引入其他PAHs单体,同时增加∑PAHs含量。毒性当量计算结果表明,米酒能有效降低豇豆体内的PAHs毒性,同时食盐处理也使豇豆体内的PAHs毒性当量降低。米酒和米醋能有效降低豇豆体内∑PAHs和二苯并(a,h)蒽的含量及其毒性当量。  相似文献   
2.
在300 mmol/L Na Cl胁迫条件下,研究了水杨酸对豇豆[Vigna unguiculata(L.)Walp]幼苗生长发育的影响。结果表明,经水杨酸处理的豇豆幼苗株高、茎粗明显提高,促进了幼苗的形态构建。并且幼苗的超氧化物歧化酶和过氧化物酶活性均显著高于对照,丙二醛含量和电解质渗透率显著或明显降低;水杨酸在适宜的施用浓度范围内能够缓解豇豆幼苗的盐害症状,提高抗盐能力,对豇豆幼苗的生长有一定的促进作用,当水杨酸的处理浓度为100~150 mg/L时,作用效果最明显。  相似文献   
3.
Productivity of maize ( Zea mays L.) legume intercrops is determined by soil, management, and environment. Planting sequence and time and N fertilization are easily controlled management factors but their effects on intercrop yields are not well understood. Maize grown in monoculture or intercropped with polebean ( Phaseolus vulgaris L.) or cowpea ( Vigna unguiculata [L.] Warp.) was studied for two growing seasons at Morgantown, WV. Crops were seeded in the following sequences: maize before legume, both at the same time, and legume before maize. Planting times were early May or mid June. Nitrogen was applied at 0 or 160 kg ha−1. Maize grain and forage, legume grain and forage, and total forage production were determined on a dry matter basis. Intercropping (average of all treatments) reduced maize grain and forage yields compared to maize in monoculture but had no effect on total forage production. However, total forage production was greatest when the seeding sequence was maize intercropped at the same time or before cowpea. Cowpea never produced grain, but forage production was almost double that of polebean. Maize produced most forage when seeded before the legumes, and the legumes produced most forage when seeded before maize. Early planting increased maize production and decreased legume production. Nitrogen increased maize grain, maize forage, and total forage yields but had not effect on legume forage production. It is concluded that maize/legume intercrops show promise for increasing forage production in temperate areas and more research on planting times and densities, weed control, harvesting and management is needed.  相似文献   
4.
Strains of Xanthomonas campestris pv. vignicola (Xcv), isolated from cowpea leaves with blight or minute pustules and collected from various geographic areas, were selected on the basis of pathological and physiological features. All strains were analyzed for genotypic markers by two methods: ribotyping with EcoRI endonuclease, and RFLP analysis with a plasmid probe (pthB) containing a gene required for pathogenicity from Xanthomonas campestris pv. manihotis. Ribotyping revealed a unique pattern for all the strains that corresponded to the previously described ribotype rRNA7. Based on polymorphism detected by pthB among Xcv strains, nine haplotypes were defined. The observed genetic variation was independent of the geographic origin of the strains and of pathogenic variation. Some haplotypes were widely distributed, whereas others were localized. In some cases, we could differentiate strains isolated from blight symptoms and pustules according to haplotypic composition. However, in most cases, no significant differences were observed. Our results and the previous pathogenic and biochemical characterizations suggest that the strains isolated from leaves with blight symptoms or minute pustules belong to the same pathovar. We provide information on pathogen diversity that can be used to identify and characterize resistant germplasm.  相似文献   
5.
【目的】豆象是危害豇豆最主要的仓储害虫。发掘豇豆抗豆象基因为抗性品种的选育,以及减少豆象对豇豆生产的危害奠定基础。【方法】以中豇1号(感)和Pant-lobia-1(抗)为亲本构建的包含282个株系的RIL群体为研究材料,利用人工接种法分别对282个株系接种绿豆象和四纹豆象,进行抗豆象表型鉴定,并利用两亲本对3 992个来源于绿豆、小豆和豇豆的SSR标记进行多态性筛选,然后利用筛选到的多态性标记对282个株系进行基因型分析,最后结合RIL群体各株系抗豆象表型鉴定数据和基因型分型数据,采用完备区间作图法(ICIM-ADD)进行抗豆象QTL定位分析,在此基础上构建遗传连锁图谱,并定位豇豆抗豆象基因。【结果】中豇1号和F1籽粒的被害率均为100%,Pant-lobia-1籽粒的被害率分别为22.5%和42.5%。推测Pant-lobia-1对绿豆象和四纹豆象的抗性均为隐性遗传;筛选到182个多态性标记,利用这些多态性标记构建了一个包含11个连锁群的豇豆遗传连锁图谱,图谱总长1 065.23 cM,相邻标记间平均遗传距离为5.85 cM;经2种豆象处理,分别在连锁群1和连锁群5上检测到2个稳定的QTL位点,暂定名为vubr1-1vubr5-1,其中vubr1-1位于标记XD11-44和HAAS_VR_2274之间,标记间的遗传距离为7.6 cM,在2种豆象处理中分别可以解释表型变异的7.16%和6.92%;vubr5-1位于标记XD1-14和CP185之间,标记间的遗传距离为2.90 cM,在2种豆象处理中分别可以解释表型变异的6.96%和6.37%。【结论】构建了一个包含11个连锁群、182个多态性标记的豇豆遗传连锁图谱,检测到2个与抗豆象相关的QTL位点。  相似文献   
6.
豇豆叶绿体微卫星标记的开发及其在近缘种的通用性研究   总被引:1,自引:0,他引:1  
潘磊  李依  郭瑞 《长江蔬菜》2014,(6):9-15
从公共数据库中下载豇豆叶绿体基因组,对其进行全序列分析,揭示豇豆cpSSR的分布特点和基序特征,进一步研究豇豆cpSSR在豆类植物中的通用性。研究结果表明,豇豆叶绿体基因组上共有52个微卫星,主要分布于LSC区,且以A/T为单碱基重复基序的微卫星为主;从豇豆的52个叶绿体微卫星中筛选出8对多态性豇豆cpSSR引物,在豌豆、利马豆和菜豆中均成功扩增,表明这些豇豆cpSSR引物在其豆类近缘种中具有较好的通用性。  相似文献   
7.
Abstract

Most of the major diseases of Hevea brasiliensis are of worldwide distribution (with the notable exception of South American leaf blight, against which strict quarantine regulations are enforced by rubber-growing countries outside the Americas to prevent the unauthorised import of Hevea) but their local severity and importance vary from one region to another. Root diseases are a serious problem nearly everywhere and regular rounds of inspection and treatment are essential while the trees are still young in order to prevent serious losses. The recently introduced prophylactic collar protectant dressings specific to each of the three main diseases are valuable aids to control.

Diseases of the tapping panel may prevent tapping or hinder bark regeneration; black stripe in particular (Phytophthora palmivora) can be very persistent and is often eradicated only by repeated therapeutic fungicidal treatment of the bark. Stem diseases, chiefly pink disease (Corticium salmonicolor), are important in certain areas in wet weather, and can lead to severe damage and even dieback in the absence of effective treatment.

Four major leaf diseases can have a debilitating effect on the tree. Abnormal leaf fall (Phytophthora spp.) is severe in India, causing the abscission of mature leaves during the monsoon rains; treatment necessitates the application of a pre-monsoon prophylactic copper spray. Secondary leaf fall, caused both by Oidium heveae and Colletotrichum gloeosporioides which infect the flushes of new leaves produced after the annual leaf change (wintering), varies greatly in severity according to local weather and cultivar. It can be controlled by repeated rounds of an appropriate prophylactic fungicide or, under certain conditions, by artificially hastening the onset of wintering (and thus of refoliation) by removing the old leaves with a contact herbicide. By far the most important leaf disease is South American leaf blight (Microcyclus ulei); it is largely responsible for the lack of a vigorous rubber industry in South America, the home of the rubber tree. It can only be effectively countered by the use of resistant cultivars.

The economic importance of the above diseases is discussed and current control measures described.  相似文献   
8.
The F2 progenies of crosses between several cowpea (V. unguiculata) lines were investigated for variation of eye pattern and seed coat colour. It was found that three (W, H, O) and five (R, P, B, M, N) major genes control eye pattern and seed coat colour, respectively. The recessive gene (GO) for restricted eye pattern enables the underlying basic white or cream seed coat colour to be observed. A similar effect is obtained with the recessive gene (rr) for colour expression. The expression of mottling (V), possibly a seed coat pattern, may for be observed when it is combined with the genes for certain eye patterns. The significance of these findings in breeding for consumer preference for specific seed coat colour is discussed.  相似文献   
9.
以豇豆种子为受体材料,研究5种落叶提取液对豇豆种子的化感作用.结果表明:不同浓度的落叶提取液对豇豆种子发芽和幼苗的生长有不同的影响,落叶提取液对种子萌发表现出高浓度抑制、低浓度促进的现象.对幼苗的生长有一定的促进作用,小叶榕、黄桷兰落叶提取液不同程度地抑制豇豆的干物质重,且随着浓度的增加,抑制作用逐渐增强;桂花落叶提取液对豇干物质重的影响呈现出低浓度促进、高浓度抑制现象.  相似文献   
10.
Drought is an important yield-reducing factor for corn and soya bean which are the two major crops in the Delaware, Maryland and Virginia (Delmarva) region of the United States. Cowpea (Vigna unguiculata L. Walp.) is primarily grown in drier regions of the world where it is one of the most drought-resistant food legumes. Field experiments were conducted in which 10 genetically diverse cowpea genotypes were evaluated for adaptability to the Delmarva area. The cowpea genotypes were grown in rain-out shelters under non-water-stressed and water-stressed conditions. The results showed that under non-water-stressed conditions cowpea genotypes California Blackeye 5, Champion and Mississippi Silver gave higher seed yields, while genotypes White Acre, Six Week Browneye and Texas Cream 8 provided lower seed yields. Genotypes California Blackeye 5 and Champion gave comparatively better seed yields under water-stressed conditions. California Blackeye 5 was the highest seed-yielding genotype under both water-stressed and non-water-stressed conditions. The highest biological yield under non-water-stressed conditions was given by genotypes Two Crop Brown, White Acre and Elite, whereas under the water-stressed condition genotypes Texas Cream 8, California Blackeye 5, and Mississippi Silver gave higher biological yield. Genotypes Quickpick Pinkeye and Elite were identified as early maturing genotypes. The harvest index (HI) varied significantly among genotypes, with Texas Cream 8 having the lowest HI. Cowpea genotypes which gave higher seed yield under water-stressed conditions could play an important role in sustaining crop production in the Delmarva region.  相似文献   
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