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1.
Peru Gopal BISWAS Yuma OHARI Uday Kumar MOHANTA Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):666
We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future. 相似文献
2.
长江水系中华绒螯蟹线粒体DNA的遗传多样性研究 总被引：3，自引：0，他引：3
本文对长江水系南京和江都地区中华绒螯蟹共61个个体的线粒体COI基因进行了限制性片段长度多态性(RFLP)分析。应用PCR技术扩增了中华绒螯蟹线粒体COI基因,选用8种能识别4碱基的限制性内切酶对该基因片段进行RFLP分析。在两个群体中共检测出8种复合单倍型,其中复合单倍型AAAAAAAA和BBBBBAAB为两个群体所共有,它的个体数所占的百分比在两个群体(南京和江都)中分别为87.9%、12.1%和50.0%、25.0%。在南京群体中,复合单倍型间的遗传距离为0.077,而在江都群体中,复合单倍型间的遗传距离为0.004～0.077；南京和江都群体的线粒体COI基因多态度(或称核苷酸多样性指数)π值分别为0.008和0.019。结果说明长江水系中华绒螯蟹具有一定的群体内遗传多样性。 相似文献
3.
通过对菱蜡蝉科Cixiidae菱蜡蝉亚科Cixiinae 9属17种昆虫的mt DNA COI基因序列进行研究,探讨DNA条形码在菱蜡蝉亚科昆虫中快速识别和准确鉴定的可行性.采用MEGA 5对序列进行比对和遗传距离分析,基于COI基因序列构建ML、MP、NJ、ME系统发育树.结果显示：属间平均遗传距离为0.133,介于0.102 ～0.147之间;属内种间平均遗传距离为0.047,介于0.025～ 0.079之间;地理种群间平均遗传距离为0.039;介于0.024～0.064之间.系统发育树显示：同属物种聚为一小支,分支置信度高达97％ ～ 100％;同一地理类群聚为一支,分支置信度高达98％ ～100％.结果表明应用基于COI基因片段的DNA条形码对菱蜡蝉亚科昆虫分类鉴定是可行的. 相似文献
4.
A. J. Cannone 《African Zoology》2013,48(2):179-190
The freshwater snails belonging to the genus Melanoides Olivier, 1804 are widespread across tropical regions of the world and endemic species have evolved in the African Lakes Malawi, Mweru and Tanganyika. The endemic Melanoides species of Lake Malawi have been investigated several times during the last century, due to their large conchological variation, but no unambiguous answer regarding the number of species has been given. The phylogenetic relationship between morphs or genetic clones of Melanoides in Lake Malawi was inferred by phylogenetic analyses of DNA sequence data from the mitochondrial genes 16S and COI. Additional sequences from GenBank were included to investigate the relationship to other morphs from different parts of the world. For the first time, a putative secondary structure was developed for a partial region of 16S in this genus to identify the variability of the secondary structure in stems and loops. The molecular analyses indicated that several genetic clones exist in Lake Malawi and that M. tuberculata is a paraphyletic taxon. It is not clear from the results whether invasions or dispersals account for the complex situation in Lake Malawi. The basal position of M. admirabilis, endemic to Lake Tanganyika, in the inferred phylogeny indicates that Africa might be the origin of the genus. The results further indicate that three major clades of Melanoides, consisting of several genetic clones, are present in Lake Malawi; one clade consisting of invasive M. tuberculata, another of native M. tuberculata and a third consisting of the M. polymorpha- complex. It appears as if the unique development of morphs within the Melanoides genus in Lake Malawi has evolved primarily by divergence of genetic clones instead of species differentiation. 相似文献
5.
Bemisia tabaci(Gennadius) is a serious pest in many cropping systems worldwide and occurs in different biotypes. The mtDNA COI gene of the 12 Bemisia tabaci (Gennadius) populations from different regions and countries were analyzed.Based on mtDNA COI sequences, their biotypes were characterized and phylogenetic relationships among these populations were established with the method of UPGMA. The results indicated the genetic similarity between those populations from Beijing, Zhengzhou, Zaozhuang, Nanjing, Shanghai, Haikou, and the B-biotype populations from California, Texas, Arizona reached 99.8-100%, which meant the nation-wide infested populations of B. tabaci in China in recent years were B-biotypes. Another population collected from Kunming of Yunnan Province showed very high similarity with Q-biotype B. tabaci from Spain and Morocco, which meant the Kunming population was Q-biotype. This is the first report on the invasion of Q-biotype into China. 相似文献
6.
烟粉虱复合种不同地理种群的遗传分化 总被引：13，自引：0，他引：13
烟粉虱Bemisia tabaci(Gennadius) 是由具有遗传分化的不同地理种群组成的复合种。本文介绍了烟粉虱不同地理种群遗传分化的最新研究进展，并在世界各国对烟粉虱核糖体ITS1(rDNA ITS1)、线粒体COI（mtDNA COI）基因大量测序的基础上，进一步分析了烟粉虱不同地理种群的遗传分化。根据mtDNA COI和rDNA ITS1基因序列分析的结果，烟粉虱不同地理种群可分为5组，即亚洲组（Asia group）、美洲组（America group）、非洲组（Africa group）、澳洲组(Australia group)、B型/地中海/中东/北非/Ms型组(Biotype B/Mediterranean/Middle Eastern/Northern Africa/Biotype Ms group)；此外，还包括3个没有特定组的种群，即乌干达（Uganda）、科特迪瓦（Ivory Coast和台湾（Taiwan）种群。地理隔离可能是造成烟粉虱不同地理种群遗传分化的最重要驱动力。许多具有入侵性或生物学优势的烟粉虱种群随着人类活动而传入新的地区，造成了严重的经济损失。有必要加强烟粉虱生物型的监测，遏制已入侵烟粉虱种群的蔓延，防止新的具有潜在危险性的烟粉虱种群传入中国。 相似文献
7.
实蝇是世界重要的检疫性害虫之一,对果蔬生产及其国际贸易具有很大的影响。而以线粒体DNA细胞色素C氧化酶亚单位I(mtDNACOI)基因的部分序列作为实蝇的DNA条形码能减少对实蝇成虫形态特征的依赖,可检测其任何虫态的样品,有助于实蝇样品的快速鉴定。本研究采用DNA条形码技术,针对采自泰国四色菊市番石榴烂果中的5头实蝇幼虫进行COI扩增测序,与生命条形码数据库(BOLD)中的序列进行比对并利用PAUP4.0软件构建了其系统进化树。根据序列分析和系统进化关系分析的结果,将5头实蝇幼虫样品鉴定为番石榴实蝇(Bactrocera correctaBezzi),并将本研究获得的2条COI序列在GenBank中注册,GenBank的登录号为HM590450和HM590451。 相似文献
8.
Francesco Frati Ilaria Negri Pietro P. Fanciulli Marco Pellecchia Vincenzo De Paola Valerio Scali Romano Dallai 《Pedobiologia》2004,48(5-6):461-468
The Gram-negative -Proteobacterium Wolbachia pipientis has been described as an obligate endosymbiont in many arthropod species, where it induces a variety of reproductive alterations, including parthenogenesis. Recently, this microorganism has also been detected in the parthenogenetic collembolan Folsomia candida. Here, we confirm the occurrence of the endosymbiont also in two Italian parthenogenetic populations of F. candida using ultrastructural (electron microscopy) and molecular (PCR screening on two bacterial genes) evidence. The strain isolated in the Italian populations has almost-identical gene sequences compared to that previously isolated in other populations of F. candida. In addition, we discovered a population of Folsomia cf. candida, which showed the presence of both males and females. This population is not infected by Wolbachia. A screening of two mitochondrial genes (COI and COII) showed that the bisexual population has high levels of genetic divergence in comparison with the parthenogenetic ones, even suggesting the possibility that it belongs to a different species. Furthermore, the remarkably high levels of genetic divergence between the two parthenogenetic populations suggests a possible influence of Wolbachia on inducing such differentiation, and, in the long term, speciation. 相似文献
9.
伴随着中国水产养殖业的快速发展,原产美国的旧金山卤虫(Artemia franciscana)被引入至我国渤海湾地区.为研究旧金山卤虫被引入中国后其基因组遗传多样性改变情况,本研究利用COI线粒体分子标记,评估旧金山卤虫在非原生栖息地,即唐山曹妃甸南堡镇(渤海湾,河北省)定殖的旧金山卤虫的遗传多样性现状.研究分析发现:... 相似文献
10.
外来有害生物的快速准确鉴定有助于提高口岸检疫效率。红翅大小蠹(Dendroctonus rufipennis)和黄杉大小蠹(D. pseudotsugae)作为大小蠹属的非中国种是我国口岸原木检疫中频繁截获的检疫性有害昆虫。本研究以mtDNA COI基因5′端和3′端序列为标记对红翅大小蠹与黄杉大小蠹的11个样本开展分子鉴定有效性评价。结果显示,当以COI基因5′端和3′端为靶标时,两种大小蠹间的平均遗传距离分别为0.181和0.144,是种内遗传距离的60.3和48倍,且种内、种间遗传距离间无重叠现象,在系统发育树上都能聚为独立的分支,表明mtDNA COI基因5′端和3′端序列均可有效区分红翅大小蠹与黄杉大小蠹。 相似文献