用台盼兰—姬姆萨染色检测家畜精子顶导反应的研究   总被引：1，自引：0，他引：1  

张嘉保  董伟 《黑龙江畜牧兽医》1992,(1):6-10

本文探讨了用台盼兰—姬姆萨染色检测家畜精子顶体反应的可行性。用肝素或钙离子载体诱发精子顶体反应。根据染色结果将精子分为四类:a)核后帽部不着色或淡青色,顶体不着色或部分紫红色(有顶体反应活精子);b)核后帽部暗青色.顶体部不着色或部分暗红色(有顶体反应死精子);c)核后帽部不着色或淡青色,顶体部紫红色(无顶体反应活精子);d)核后帽部暗青色,顶体部暗紫红色(无顶体反应死精子)。有顶体反应活精子百分率与仓鼠卵穿透率呈强正相关。从而证明台盼兰—姬姆萨染色是检测家畜精子顶体反应的有效手段,并能预测获能处理后精子的受精能力。  相似文献   

影响精液冷冻和人工授精效果的因素     

吴结革  茆达干 《动物医学进展》2003,24(5):48-50

通过对精液冷冻保存的细胞反应原理的阐述 ,指出冷冻保护剂甘油对精液保存具有利弊效应 ,提出精子膜脂组成的差异使得不同品种的精子对冷冻损伤的易感性不同。雌性生殖道解剖结构的品种差异 ,精子形态 ,精子运行机制的细微差异 ,人工授精时间及精子的运行能力 ,采精方式等因素对精液冷冻保存和人工授精的成功有决定性作用。研究精子质膜的生物学特性可解决低活力精子的问题 ,然而这并不能解决冷冻后精子质量的个体差异。对精细胞基因组的研究可以找出这些个体的遗传差异。因此 ,冷冻精子和精原细胞 (用于细胞外注射 )的差异已经成为完整基因组问题。  相似文献   

维生素E对绵羊鲜精及冻精精液品质的影响   总被引：5，自引：0，他引：5  

罗海玲  贾志海  朱士恩  丁金芝 《中国草食动物》2004,24(5):14-16

日粮中添加维生素E可以提高绵羊鲜精的活率 ,对照组和试验组的活率分别为 0 72± 0 0 7和 0 78± 0 0 6(P <0 0 5 ) ,显著改善鲜精精液品质。采用两步稀释法在绵羊冷冻精液稀释液中添加维生素E ,可以极显著降低冷冻对精子顶体的冷刺激损害程度 ,试验组的活率 ( 0 43 5± 0 0 2 0 )极显著高于对照组 ( 0 3 65± 0 0 2 6) (P <0 0 1) ,精子顶体的总异常率试验组 ( 3 3 72 % )极显著低于对照组 ( 4 4 3 5 % ) (P <0 0 1) ,其中试验组顶体膨胀率和脱落率与对照组分别为 1 2 8%±0 5 8%、2 3 0 8%± 1 45 %与 2 68%± 0 5 9%、2 8 96%± 3 14 %。冷冻精液品质显著改善。  相似文献   

水牛附睾尾精子的体外受精     

谢英  单天锡  李跃民  张家骅 《中国兽医学报》2004,24(2):190-192

采用肝素诱导获能 ,比较了 TAL P液和 BO液处理水牛附睾尾精子进行体外受精和细管冷冻精液体外受精的效果。结果表明 ,用 BO液和 TAL P液分别处理水牛附睾尾精子 ,受精后的卵裂率分别为 4 6 .6 7%和 5 3.73% ,发育率分别为 2 1.6 7%和 2 6 .87% ,其受精效果差异不显著。综合 2种方法 ,水牛附睾尾精子的受精率为 5 7.14 % ,受精后的卵裂率为 5 0 .39% ;发育率相对于培养卵为 2 4 .4 1% ,相对于卵裂卵为 4 8.4 4 % ;与细管冷冻精液 (5 6 .0 0 % ,5 4 .31% ,2 6 .72 % ,4 9.2 1% )相比 ,差异不显著。形态学观察还表明 ,用保温干储的方法可获得活率好、存活时间长的附睾尾精子。试验结果说明水牛附睾尾精子用于体外受精可以得到与细管冷冻精液相当的效果。  相似文献   

Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats          下载免费PDF全文

M Narasimhaiah  A Arunachalam  S Sellappan  VK Mayasula  PR Guvvala  SK Ghosh  V Chandra  J Ghosh  H Kumar 《Reproduction in domestic animals》2018,53(3):644-654

Trace minerals feeding had significant effects on sperm production and fertility with better absorption and proper utilization within the body for optimum reproductive function. Several studies have shown that more influenced trace elements in the diets of animals are copper (Cu) and zinc (Zn). Bucks showing deficiency of this mineral might affect the quality of semen production which in turn would affect the fertility. This experiment was thus designed to test the effects of organic Cu and Zn supplementation on antioxidants enzyme activities and sperm functional attributes in fresh semen of bucks. Forty bucks (n = 40, Aged 5 months) were assigned to ten groups of four animals in each group, supplemented (for a period of 8 months) with different levels of organic Zn: 20 mg (T2), 40 mg (T3) and 60 mg (T4), organic Cu: 12.5 mg (T5), 25 mg (T6), 37.5 mg (T7) and combined organic Zn and Cu: 20 + 12.5 mg (T8), 40 + 25 mg (T9), 60 + 37.5 mg (T10), respectively, per kg dry matter and no additional mineral diet (control; T1). One hundred and sixty semen samples were collected through electro‐ejaculator and analysed for sperm quantity, quality, acrosome intactness and plasma membrane integrity and correlated with the catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase enzyme activities in seminal plasma. The results indicated organic Cu and zinc supplemented bucks produced more sperm cells, had higher sperm concentrations, maintained higher (p < .01) sperm livability, plasma membrane and acrosome integrities, more motility and velocity. The increased antioxidant enzyme activities, reduced oxidative stress and lowered lipid peroxidation were positively correlated (p < .05) with the sperm functional attributes. In conclusion, organic Cu and Zn supplement to male goats showed protective roles against oxidative damage and maintained better fresh semen characteristics.  相似文献   

Single layer centrifugation of stallion spermatozoa consistently selects the most robust spermatozoa from the rest of the ejaculate in a large sample size     

J. M. MORRELL  H. RODRIGUEZ‐MARTINEZ  A. JOHANNISSON 《Equine veterinary journal》2010,42(7):579-585

Reasons for performing study: An improvement in sperm quality after single layer centrifugation (SLC) has been seen in previous studies using small sample sizes (for example, n = 10 stallions). There is a need to investigate whether this improvement is repeatable over several breeding seasons with a larger number of stallions (n ≥ 30 stallions). Objective: To make a retrospective analysis of the results of SLC performed on more than 250 sperm samples (176 ejaculates) from 31 stallions in 3 consecutive breeding seasons. Methods: Sperm quality (motility, proportion of morphologically normal spermatozoa and the proportion of spermatozoa with undamaged chromatin) was assessed before and after SLC. Results: All parameters of sperm quality examined were significantly better in sperm samples after SLC than in their unselected counterparts (P<0.001 for each parameter). The yield of spermatozoa obtained after SLC was influenced by the type of extender used and also by the concentration of spermatozoa in the original ejaculate, with fewer spermatozoa being recovered when the loading dose contained a high concentration of spermatozoa. The optimal concentration was approximately 100 × 10⁶/ml. Sperm concentration in the samples loaded on to the colloid influenced the sperm yield while the type of semen extender affected sperm quality and survival. Furthermore, the scaled‐up SLC method was found to be suitable for use with a range of ejaculates, with similar sperm kinematics being observed for standard and scaled‐up preparations. Conclusions: SLC consistently improved the quality of stallion sperm samples from a large number of ejaculates. The method could be scaled‐up, allowing larger volumes of ejaculate to be processed easily from a wide range of stallions.  相似文献   

New technique of sex preselection for increasing female ratio in boar sperm model     

Yoo-Jin Park  Ki-Jin Kwon  Won-Hee Song  Won-Ki Pang  Do-Yeal Ryu  Md Saidur Rahman  Myung-Geol Pang 《Reproduction in domestic animals》2021,56(2):333-341

In this study, we tried to optimize the porcine semen extender conditions to maximize the differences between live X chromosome-bearing (X) spermatozoa and to Y chromosome-bearing (Y) spermatozoa without a decline in the fertility rate at different pH conditions during storage. We observed the viability of X and Y boar spermatozoa in acidic (pH 6.2), original (pH 7.2), and alkaline condition (pH 8.2) for 5 days to investigate the effect of storage conditions on the X to Y spermatozoa ratio. The functional parameters of spermatozoa were also examined to evaluate sperm quality. Sperm motility was preserved at pH 7.2 and pH 6.2 for 3 days, while sperm motility at pH 8.2 decreased significantly after 2 days. Non-capacitated spermatozoa increased while capacitated spermatozoa decreased during storage. Sperm viability decreased significantly duration-dependent under all pH conditions, but there was no significant difference during storage at pH 6.2 and 7.2. The X: Y ratio of live spermatozoa in acidic condition was maximized (1.2:1) without affecting the sperm function and fertility-related protein expression after 2 days compared to original conditions. Moreover, insemination of sows using acidic extender increased the number of female pups on days 1 and 2 of preservation. These results indicate that the production of female offspring may increase when acidic BTS is used for 2 days without affecting the success rate of AI. Above all, this method is simple and economical compared to other methods.  相似文献   

Influence of Boar Seminal Plasma on the Distribution of Spermatozoa in the Genital Tract of Gilts     

Sven Viring  Stig Einarsson 《Acta veterinaria Scandinavica》1980,21(4):598

The main purpose of the present study was to investigate whether boar seminal plasma affects the transport of spermatozoa in the genital tract of oestrous pigs or not, with special reference to the sperm transport into the oviducts. Altogether 17 gilts were used in three experiments.Experiment I. In nine gilts one uterine horn was injected surgically with 10¹⁰ spermatozoa suspended in seminal plasma and the other uterine horn with 10¹⁰ spermatozoa suspended in TESNaK-glucose buffer solution. The sperm deposition was performed under general anaesthesia. The gilts were slaughtered 1–2 or 4–6 h after insemination. The genital tract was removed and the numbers of spermatozoa determined in oviducts and in uterine horns.Experiment II. The insemination doses were prepared exactly as in Experiment I. Approx. 24 h before insemination Polyvinylchloride cannulas were inserted into the uterine lumen of the horns, drawn via the midventral incision at linea alba subcutaneously to cutaneous incisions ventral to the vulva opening. One cannula was placed in each uterine horn. At standing heat the insemination doses were slowly injected through the cannulas. The gilts were slaughtered 1 h after insemination and the numbers of spermatozoa within the genital tract were counted.Experiment III. In three gilts under general anaesthesia the uterine horns were ligated 10 cm from the uterotubal junction. The semen doses (containing 2 × 10⁹ spermatozoa), prepared as in Experiment I, were deposited into the uterine horns anterior to the ligatures through a cannula. The gilts were slaughtered 1 h after insemination, and the numbers of spermatozoa within the oviducts and ligated part of the uterine horns were counted.In all three experiments more spermatozoa were, on average, recovered in the oviducts connected to uterine horns inseminated with spermatozoa suspended in seminal plasma. In Experiments I andII this was the case for 10 of 14 gilts and in Experiment III for all the three gilts. It is therefore suggested that boar seminal plasma pro¬motes sperm transport into the oviduct of oestrous pigs. The back¬ground mechanism for this is discussed.  相似文献   

黑龙江茴鱼(Thymallus arcticus grubei Dybowski)精子活力的观察   总被引：5，自引：2，他引：3  

韩英  张澜澜  徐革峰  牟振波  赵吉伟 《水产学杂志》2008,21(1):9-14

用BSS和水两种激活剂对黑龙江茴鱼精子活力进行测定,并在4℃和14℃(室温)条件下,对精原液和用ASP稀释的精液分别进行保存试验.试验结果显示,用BSS和水均可激活黑龙江茴鱼精子,其精子活率分别可达100%和98%.14℃条件下,用BSS激活黑龙江茴鱼精子平均寿命为72.1s,A级(快速)运动时间平均为14.1s;用水激活精子平均寿命为67.2s,A级运动时间平均为12.5s.不同温度保存的精子寿命和快速运动时间也有显著差异:精原液4℃保存2h后精子失去A级运动,其激活率在70%以上,保存6h后激活率小于20%;精原液14℃保存1.5h后精子失去A级运动,其激活率大于70%,4h后激活率小于20%.经ASP(pH分别为7.0、7.8、8.0、8.5、9.0)稀释的精液,在4℃和14℃条件下,BSS和water对精子的激活率均小于20%,且精子均呈现C、D级运动,1.5h后各保存组激活率为0.试验结果表明,BSS对黑龙江茴鱼的精子有良好的激活效果;精原液4℃保存效果好于14℃,在4℃保存条件下2h以内使用,可以得到理想的结果;精液不宜用ASP稀释保存.  相似文献   

超低温冷冻对日本鳗鲡精子酶活性的影响   总被引：2，自引：1，他引：1  

黄晓荣  章龙珍  庄平  江琪  刘鉴毅  姚志峰  张涛  冯广朋  赵峰 《海洋渔业》2008,30(4)

研究超低温冷冻保存(-196℃)对日本鳗鲡精子内总ATP酶、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)等酶活性的影响。运用试剂盒分别测定了冷冻前后日本鳗鲡精子内酶活性的变化。结果表明,经过超低温冷冻保存后,日本鳗鲡精子的活力下降,精子内GR活性显著升高(P<0.05),酶活性从冻前的358.52±45.65 U/L上升到646.30±70.30 U/L;其它几种酶的活性均显著下降(P<0.05),总ATP酶、CK和SDH的活性分别从冻前的3.14±0.61 U/ml、17.10±3.51 U/ml和32±5.94 U/ml下降到1.83±0.43 U/ml、7.33±1.74 U/ml和21±1.41 U/ml,LDH、SOD和CAT活性分别从冻前的2 266.67±313.25 U/L、220.47±32.94 U/ml和48.51±5.94U/ml下降到1 195.91±198.51 U/L、84.16±22.11 U/ml和21.8±4.14 U/ml。超低温冷冻对日本鳗鲡精子酶活性和精子活力均有较大影响。  相似文献