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| 超低温冷冻对日本鳗鲡精子酶活性的影响 | |
| 引用本文： | 黄晓荣,章龙珍,庄平,江琪,刘鉴毅,姚志峰,张涛,冯广朋,赵峰.超低温冷冻对日本鳗鲡精子酶活性的影响[J].海洋渔业,2008,30(4). |
| 作者姓名： | 黄晓荣 章龙珍 庄平 江琪 刘鉴毅 姚志峰 张涛 冯广朋 赵峰 |
| 作者单位： | 1. 中国水产科学研究院东海水产研究所,上海,200090;上海海洋大学生命科学与技术学院,上海,200090 2. 中国水产科学研究院东海水产研究所,上海,200090;上海海洋大学生命科学与技术学院,上海,200090;上海市高校水产养殖学E-研究院,上海,200090 3. 中国水产科学研究院东海水产研究所,上海,200090 |
| 基金项目： | 国家科技基础条件平台项目 ， 中央级公益性科研院所基本科研业务费专项资金资助项目 ， 上海市高校水产养殖学E-研究院建设项目 |
| 摘 要： | 研究超低温冷冻保存(-196℃)对日本鳗鲡精子内总ATP酶、肌酸激酶(CK)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)等酶活性的影响。运用试剂盒分别测定了冷冻前后日本鳗鲡精子内酶活性的变化。结果表明,经过超低温冷冻保存后,日本鳗鲡精子的活力下降,精子内GR活性显著升高(P<0.05),酶活性从冻前的358.52±45.65 U/L上升到646.30±70.30 U/L;其它几种酶的活性均显著下降(P<0.05),总ATP酶、CK和SDH的活性分别从冻前的3.14±0.61 U/ml、17.10±3.51 U/ml和32±5.94 U/ml下降到1.83±0.43 U/ml、7.33±1.74 U/ml和21±1.41 U/ml,LDH、SOD和CAT活性分别从冻前的2 266.67±313.25 U/L、220.47±32.94 U/ml和48.51±5.94U/ml下降到1 195.91±198.51 U/L、84.16±22.11 U/ml和21.8±4.14 U/ml。超低温冷冻对日本鳗鲡精子酶活性和精子活力均有较大影响。 |
| 关 键 词： | 超低温冷冻 日本鳗鲡 精子 酶活性 |
Cryopreservational effects on the spermatic enzyme's activities of Japanese ell (Anguilla japonica) |
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| HUANG Xiao-rong,ZHANG Long-zhen,ZHUANG Ping,JIANG Qi,LIU Jian-yi,YAO Zhi-feng,ZHANG Tao,FENG Guang-peng,ZHAO Feng.Cryopreservational effects on the spermatic enzyme''s activities of Japanese ell (Anguilla japonica)[J].Marine Fisheries,2008,30(4). | |
| Authors: | HUANG Xiao-rong ZHANG Long-zhen ZHUANG Ping JIANG Qi LIU Jian-yi YAO Zhi-feng ZHANG Tao FENG Guang-peng ZHAO Feng |
| Abstract: | Present paper dealed with the study concerning the effects of cryopreservation(-196 ℃) on the enzyme's activities(including total ATP,SDH,LDH,SOD,CAT and GR) in spermatozoa of Japanese eel(Anguilla japonica).By means of reagent box,the changes of enzyme's activities of spermatozoa mentioned above have been respectively analysed.The results showed: the activities of spermatozoa dropped after cryopreservation,GR activities increased significantly(P<0.05),the activities added from 358.52±45.65 U/L to 646.30±70.30 U/L,other enzyme's activities descended significantly(P<0.05).The activities of total ATP,CK,SDH dropped from 3.14±0.61 U/ml,17.10±3.51 U/ml and 32±5.94 U/ml to 1.83±0.43 U/ml,7.33±1.74 U/ml and 21±1.41 U/ml respectively,the activities of LDH,SOD and CAT declined from 2266.67±313.25 U/L,42.65±1.56 U/ml and 48.51±5.94 U/ml to 1195.91±198.51 U/L,84.16±22.11 U/ml and 21.8±4.14 U/ml respectively.Cryopreservation had a great effect on the enzyme's activities and spermatozoa's energy of Anguilla japonica. |
| Keywords: | Cryopreservation Anguilla japonica spermatozoa enzyme's activities |
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