| 甲壳素生产废水提取虾青素及水解蛋白的工艺研究 |
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| 引用本文： | 刘汉文,王爱民,陈洪兴,张其林.甲壳素生产废水提取虾青素及水解蛋白的工艺研究[J].饲料工业,2011,32(14). |
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| 作者姓名： | 刘汉文 王爱民 陈洪兴 张其林 |
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| 作者单位： | 盐城工学院化学与生物工程学院,224051 |
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| 摘 要： | 以小龙虾壳为原料,按酸碱交替法生产的甲壳素,蛋白质和虾青素等物质流失于废水中,采用壳聚糖作絮凝剂,可将虾青素与蛋白质絮凝沉淀。试验结果表明:调节pH值6.0,每升废水中添加40 ml 1%壳聚糖醋酸水溶液,静置3 h,废水中溶液的透光率最低,絮凝物蛋白质回收率达88.6%。使用几种蛋白酶水解絮凝物,其中风味蛋白酶水解度最大,在初始pH值6.5,水解温度55℃,试验确定:选用风味蛋白酶1.5 g/kg絮凝物,固液比1:2时,保温酶解时间4 h,虾青素提取率可达4.49%。该条件下絮凝物蛋白质的水解度为23.8%。提取虾青素并将水解后的蛋白浓缩,喷雾干燥,制成水解动物蛋白,产品不苦,具有良好的风味。
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| 关 键 词： | 小龙虾壳 壳聚糖 絮凝 酶解 虾青素 水解蛋白 水解度 |
Research on the extraction of astaxanthin and HAP in wastewater after crayfish shell processing |
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| Liu Hanwen,Wang Aimin,Chen Hongxing,Zhang Qilin.Research on the extraction of astaxanthin and HAP in wastewater after crayfish shell processing[J].Feed Industry,2011,32(14). |
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| Authors: | Liu Hanwen Wang Aimin Chen Hongxing Zhang Qilin |
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| Institution: | Liu Hanwen,Wang Aimin,Chen Hongxing,Zhang Qilin |
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| Abstract: | The shortcoming for the acid-base alternative to produce chitin from the crayfish shell was that the protein and astaxanthin were lost in the wastewater.In the current study,the chitosan was used as flocculant and the astaxanthin and protein was coagulated and sedimentated easier.The results showed that the OD reached lowest and the recycled rate reached the maximum at 88.6% when the 40 ml 1% chitosan acetic acid solution was added into the solution(pH 6.0) and stood for 3 h.The enzyme activity of several e... |
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| Keywords: | crayfish shell chitosan flocculation enzymatic hydrolysis astaxanthin HAP DH |
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