| 中国南方地区荷斯坦奶牛BoLA-DRB3.2的RFLP分析 |
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| 引用本文： | 李树春,杨章平,武秀香,王小龙.中国南方地区荷斯坦奶牛BoLA-DRB3.2的RFLP分析[J].中国牛业科学,2006,33(1):5-8. |
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| 作者姓名： | 李树春 杨章平 武秀香 王小龙 |
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| 作者单位： | 扬州大学,动物科学与技术学院,江苏,扬州,225009 |
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| 基金项目： | 江苏省科技厅推广项目;江苏省科技厅推广项目 |
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| 摘 要： | 用PCR-RFLP方法检测中国南方地区奶牛BoLA-DRB3.2的RFLP多态性。选用RsaI、HaeIII和Bst YI酶切PCR扩增产物,14%PAGE分离酶切片段。HaeIII位点检测到6种等位基因,RsaI位点检测到11种等位基因,而在BstYI位点仅检测到4种等位基因。结果表明BoLA-DRB3.2的多态性极其丰富。PCR-RFLP是BoLA-DRB3分型的一种快速而有效的方法。
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| 关 键 词： | 荷斯坦奶牛 遗传多态性 |
| 文章编号： | 1001-9111（2006）01-0005-04 |
| 收稿时间： | 2005-09-25 |
| 修稿时间： | 2005年9月25日 |
Restriction Fragment Length Polymorphism (RFLP) in the Exon 2 of the BoLA-DRB3 Gene in China Holstein Cows of the Southern China |
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| LI Shu-chun,YANG Zhang-ping,WU Xiu-xiang,WANG Xiao-long.Restriction Fragment Length Polymorphism (RFLP) in the Exon 2 of the BoLA-DRB3 Gene in China Holstein Cows of the Southern China[J].China Cattle Science,2006,33(1):5-8. |
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| Authors: | LI Shu-chun YANG Zhang-ping WU Xiu-xiang WANG Xiao-long |
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| Institution: | College of Animal Science and Technology, Yangzhou University, Yangzhou Jiangsu 225009, China |
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| Abstract: | The genetic diversity of the exon2 of BoLA-DRB3(BoLA-DRB3.2) in China Holstein cows of the South China was investigated by polymerase chain reactionrestriction fragment length polymorphism(PCR-RFLP).After digestion with restriction endonucleases,products were separated with 14%PAGE.6,4 and 11 RFLP patterns were found with enzymes HaeIII,BstYI and RsaI respectively.The result demonstrated that the BoLA-DRB3.2 locus was highly polymorphic in China dairy cattle.PCR-RFLP may therefore be a rapid and effective method for DRB3 typing in dairy cattle. |
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| Keywords: | PCR-RFLP BoLA-DRB3 2 |
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