| 抗旋毛虫循环抗原单克隆抗体的制备及初步应用 |
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| 引用本文： | 贾世玉,刁有祥.抗旋毛虫循环抗原单克隆抗体的制备及初步应用[J].中国兽医学报,1995,15(1):70-73. |
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| 作者姓名： | 贾世玉 刁有祥 |
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| 作者单位： | 济南军区军事医学研究所 |
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| 摘 要： | 为评价单克隆抗体用于检测旋毛虫循环抗原（ＣＡ）的应用价值，作者利用淋巴细胞杂交瘤技术制备了４个抗旋毛虫ＣＡ的杂交瘤细胞系，建立了以单克隆抗体双抗体夹心ＥＬＩＳＡ检测ＣＡ的方法，并初步应用于动物血清中ＣＡ的检测。应用该法检测旋毛虫病猪血清的阳性率为７２．１％（３１／４３）。６０头健康猪、３０头感染猪囊虫和３０头感染弓形虫的猪均为阴性。对流行地区河南邓县３４头和湖北囊樊１３４头屠宰猪进行流行病学调查，
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| 关 键 词： | 单克隆抗体 循环抗原 旋毛虫 双抗体夹心 ELISA |
Preparation of Monoclonal Antibodies against CirculatingAntigen of Trichinella spiralis and Its Preliminary Appli- cation |
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| Jia Shiyu ,Diao Youxiang ,Fu Xinglun ,Liu Ruitian.Preparation of Monoclonal Antibodies against CirculatingAntigen of Trichinella spiralis and Its Preliminary Appli- cation[J].Chinese Journal of Veterinary Science,1995,15(1):70-73. |
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| Authors: | Jia Shiyu Diao Youxiang Fu Xinglun Liu Ruitian |
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| Abstract: | In the present study,a trial was made to evaluate the monoclonal anti-body(McAb ) produced as tool in the detection of circulating antigen(CA)ofTrichinella spiralis. Four hybridomas secreting McAbs against the CA of T. spiraliswere established. The McAbs secreted by F1 , F2 and G5 were IgG1, and that by H8 wasIgM. The titers of F1, F2 and G5 ascites were 1 :160 000,and H8 was 1 :1 000. TheMcAbs secreted by F2and G5were specific to T. spiralis ,while secreted by both F1 andH8 could react with Cysticercus cellulosae. The McAb secreted by F2 was labelled withhorseradish peroxidase(HRP). The labelled McAb was used to detect CA by sandwich ELISA method. Sera of the infected pigs with T. Spiralis(confirmed by musclebiopsy ) were examined for CA of this parasite. The positive rate was 72.1%(31/43). Control serum specimens obtained from 60 healthy swine were all negative. In anothergroup of 60 swine sera, 30 with cysticercosis and 30 with toxoplasmosis, were all nega-tive too. A survey was made on slaughtered pigs naturally infected with T. spiralis inDengzhou and Xiangfan. A total of 168 pigs were examined and the average prevalencerate by CA was 9. 52%(16/168); and 68.8/(11/16) of them was also anti-trichinellaantibody positive. Thirty mice were orally infected with T. spiralis larvae. Their serawere collected at different periods,i.e.4,7,11,18 and 28 days post infection for thedetection of CA which was done by the sandwich ELISA, CA could be detected on day 4post infection, the positive rate was 60%( 18/30);while on day 7 and 11 it was allclearly identified(30/30);on day 18 it could be detected but at a lesser OD reading;however on the 28th day the positive rate dropped to 13.3/(4/30).This study con-firmed that McAb may be a valuable tool in the detection of CA,especially in the earlydiagnosis of trichinosis,and it is convenient for epidemiological survey in field. |
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| Keywords: | monoclonal antibody circulating antigen Trichinella spiralis doubleantibody sandwich ELISA |
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