| 应用单克隆抗体鉴别水稻白叶枯病菌血清型的研究 |
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| 引用本文： | 朱华,黄奔立,胡广淦,李清铣.应用单克隆抗体鉴别水稻白叶枯病菌血清型的研究[J].扬州大学学报(农业与生命科学版),1992(4). |
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| 作者姓名： | 朱华 黄奔立 胡广淦 李清铣 |
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| 作者单位： | ZHU Hua;HUANG Ben-li;HU Guang-gan;LI Qing-xian Dept. of Plant Protection，Jiangsu Agric. Coll，Yangzhou 225001 |
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| 摘 要： | 从已建立的15株稳定分泌抗水稻白叶枯病原细菌的单抗杂交瘤细胞株中筛选出4株,用其抗体采用琼脂双扩散和酶联免疫吸附试验检测了作者从采集的病叶分离和从各地收集的水稻白叶枯病菌菌株63株,将其分为4个血清型,其结果与常规多抗血清划分的血清型一致.并运用免疫电泳和琼脂双扩散试验比较了各血清型之间的差异,其结果表明,单抗较多抗特异性强,在鉴别血清型方面较优越.
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| 关 键 词： | 单克隆抗体 水稻 白叶枯病 血清型 |
STUDIES ON THE IDENTIFICATION OF SEROTYPES OF XANTHOMONAS CAMPESTRIS PV.ORYZAE USING THE MONOCLONAL ANTIBODIES |
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| ZHU Hua,HUANG Ben-li,HU Guang-gan,LI Qing-xian Dept. of Plant Protection,Jiangsu Agric. Coll,Yangzhou.STUDIES ON THE IDENTIFICATION OF SEROTYPES OF XANTHOMONAS CAMPESTRIS PV.ORYZAE USING THE MONOCLONAL ANTIBODIES[J].Journal of Yangzhou University:Agricultural and Life Science Edition,1992(4). |
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| Authors: | ZHU Hua HUANG Ben-li HU Guang-gan LI Qing-xian Dept of Plant Protection Jiangsu Agric Coll Yangzhou |
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| Institution: | ZHU Hua,HUANG Ben-li,HU Guang-gan,LI Qing-xian Dept. of Plant Protection,Jiangsu Agric. Coll,Yangzhou 225001 |
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| Abstract: | Among the 15 hybridoma cell lines secreting stably the monoclonal antibodies (MCAbs) against Xanthomonas campestris pv. oryzae, 4 cell lines were selected and were applied to react to with the 63 strains of X campestris pv. oryzae isolated from the country by means of Ouchterlony Double Diffu- sion Test (ODD) and Indirect Enzyme-linked Immunoassay (ELISA). According to their reaction, the 63 strains could be grouped into 4 serotypes. This result from the reaction among the McAbs and strains was same as that from the reaction among the polyclonal antibodies and the strains. The difference among the serotypes was compared by the ODD and Immunoelectrophoresis (IE), The result showed that the McAbs were higher specific and more effective than thc polyclonal antibodies in the identification of serotypes. |
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| Keywords: | Monoclonal antibodies (McAbs) Xanthomonas campestris pv oryzae Serotypes |
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