| H5亚型禽流感病毒血凝素单克隆抗体的制备及鉴定 |
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| 引用本文： | 但汉并,刘芳芳,黄红亮,金梅林.H5亚型禽流感病毒血凝素单克隆抗体的制备及鉴定[J].动物医学进展,2006,27(8):67-69. |
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| 作者姓名： | 但汉并 刘芳芳 黄红亮 金梅林 |
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| 作者单位： | 华中农业大学动物医学院动物病毒室,湖北武汉,430070 |
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| 摘 要： | 以禽流感病毒A/Chicken/Hubei/327/2004(H5N1)免疫Balb/c小鼠,将免疫鼠脾细胞与SP2/0骨髓瘤细胞融合,用血凝抑制试验筛选细胞培养上清,采用有限稀释法对阳性孔进行克隆,3次克隆后获得7株能稳定分泌抗H5亚型禽流感病毒血凝素单克隆抗体的杂交瘤细胞株,分别命名为1C4,1D4,1E12,2E11,4C12,4G2和5E12。细胞培养上清HI效价为24~27,腹水HI效价可达210~218。所有单抗与禽流感H7和H9亚型标准血凝抗原,新城疫病毒和鸡传染性支气管炎病毒无交叉反应。在细胞上的中和试验显示具有较高的中和效价,获得的单克隆抗体可在禽流感流行病学的监测中发挥重要作用。
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| 关 键 词： | 禽流感病毒 血凝素 单克隆抗体 |
| 文章编号： | 1007-5038（2006）08-0067-03 |
| 收稿时间： | 2006-05-13 |
| 修稿时间： | 2006年5月13日 |
Preparation and Identification of Monoclonal Antibodies Against Haemagglutinin of Subtype H5 Avian influenza virus |
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| DAN Han-bing,LIU Fang-fang,HUANG Hong-liang,JIN Mei-lin.Preparation and Identification of Monoclonal Antibodies Against Haemagglutinin of Subtype H5 Avian influenza virus[J].Progress In Veterinary Medicine,2006,27(8):67-69. |
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| Authors: | DAN Han-bing LIU Fang-fang HUANG Hong-liang JIN Mei-lin |
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| Institution: | Laboratory of Animal Virology, College of Veterinary Medicine, Hnazhong Agricultural University, Wuhan , Hubei, 430070, China |
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| Abstract: | Balb/c mice were immunized with the purified AIV antigens.Splenocytes from the immunized mice were fused with SP2/0 myeloma cells.Positive hybridoma clones were screened by hemagglutination inhibition(HI),and limited dilution method was performed to subclone the positive clones.After three cycles of subcloning,seven McAbs against the hemagglutinin of H5-AIV were obtained,designated as 1C4,1D4,1E12,2E11,4C12,4G2 and 5E12.The HI titers of culture supernatant and ascites were 2~(4)-2~(7) and 2~(10)-2~(18).The seven McAbs had good specificity to H5 subtype of avian influenza virus (H5-AIV).No cross-reactions were found when H7 amd H9 subtypes of avian influenza virus,Newcastle disease virus(NDV) and Avian infectious bronchitis virus(IBV) were tested.All McAbs had high neutralization titers. |
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| Keywords: | Aivan influenza virus(AIV) hemagglutinin monoclonal antibody(McAb) |
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