Complete mitochondrial DNA sequence of Atlantic horse mackerel Trachurus trachurus and molecular identification of two commercially important species T. trachurus and T. japonicus using PCR-RFLP |
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| Authors: | Yasuharu Takashima Takami Morita Michiaki Yamashita |
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| Institution: | National Research Institute of Fisheries Science, Fukuura, Yokohama 236-8648, and;Center for Food Quality, Labeling and Consumer Services Headquarter, Shintoshin, Saitama 330-9731, Japan |
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| Abstract: | ABSTRACT: To characterize and identify mitochondrial DNA (mtDNA) nucleotide sequence variation in two commercially important Trachurus species, Trachurus trachurus and T. japonicus , the complete mtDNA sequence of T. trachurus was determined. The T. trachurus mtDNA consists of 16 559 bp, containing 22 transfer RNA (tRNA) genes, two rRNA genes, and 13 protein-coding genes. Comparing the mtDNA nucleotide sequences of the Trachurus species, a polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) method was developed to differentiate these two commercially important species. The primer pair Lt1-ND5 and Ht1-ND5, corresponding to ND5 , was designed to amplify a 360-bp fragment. Following digestion with Eco RI, the PCR product for T. japonicus resulted in 93- and 267-bp fragments, while T. trachurus lacked a restriction site for Eco RI. In contrast, after digestion with Hin fI, the T. trachurus PCR product yielded 44-, 84-, and 232-bp fragments, while the T. japonicus product was not digested. The PCR-RFLP analysis established in the present study was useful for identifying T. trachurus and T. japonicus . |
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| Keywords: | Atlantic horse mackerel food labeling mitochondrial DNA RFLP species identification Trachurus trachurus |
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