| 草甘膦高抗菌株的分离、鉴定及生理生化特性研究 |
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| 引用本文： | 李海红,宛煜嵩,金芜军,王英典,林敏.草甘膦高抗菌株的分离、鉴定及生理生化特性研究[J].中国农业科技导报,2009,11(2):69-72. |
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| 作者姓名： | 李海红 宛煜嵩 金芜军 王英典 林敏 |
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| 作者单位： | 1.北京师范大学生命科学学院, 北京 100875,2.中国农业科学院生物技术研究所, 北京 100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 从采自新疆的土壤中分离到一株草甘膦抗性菌株P23，该菌株能够耐受200 mmol/L的草甘膦,可在最高含350 mmol/L草甘膦的培养基中生长。在草甘膦浓度为100～200 mmol/L之间时，菌株生长迅速，最适pH为7.0,最适生长温度为37℃，具有氨苄青霉素、卡那霉素抗性。用16S rDNA通用引物，经PCR扩增、测序得到P23的16S rDNA序列，该序列在GenBank的登录号为FJ374126。在NCBI中经BLAST后发现与苍白杆菌属(Ochrobactrum)的16S rDNA序列相似性达到99%。结合各项生理生化指标鉴定结果将菌株命名为人苍白杆菌P23（Ochrobactrum anthropi P23）。
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| 关 键 词： | 草甘膦 筛选 鉴定 16S rDNA 人苍白杆菌 |
| 收稿时间： | 2009-02-18 |
| 修稿时间： | 2009-03-19 |
Isolation, Identification and Characterization of a Glyphosate-tolerant Bacterial Strain |
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| LI Hai-hong,WAN Yu-song,JIN Wu-jun,WANG Ying-dian,LIN Min.Isolation, Identification and Characterization of a Glyphosate-tolerant Bacterial Strain[J].Journal of Agricultural Science and Technology,2009,11(2):69-72. |
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| Authors: | LI Hai-hong WAN Yu-song JIN Wu-jun WANG Ying-dian LIN Min |
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| Institution: | 1.College of Life Sciences,Beijing Normal University, Beijing 100875|2.Biotechnology Research Institute, Chinses Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | A bacterial strain P23, which can tolerate 200 mmol/L glyphosate and grow in highest concentration of glyphosate 350 mmol/ L in the medium, was isolated from soil of Xinjiang. The cell grows well in the medium with 100~200 mmol/L glyphosate, the optimal pH is 7.0 and the optimal growth temperature is 37℃. It has resistance to ampicillin, kanamycin.Using the universal primers, the 16S rDNA of P23 was amplified by PCR and sequenced. Compared with the published nucleotide sequence of 16S rDNA in NCBI, the 16S rDNA of P23 showed 99% homology with Ochrobactrum sp. According to the morphological, physiological and biochemical characteristics, the strain is identified as Ochrobactrum anthropi P23. |
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| Keywords: | 16SrDNA |
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