Bacteriocin Activity of Enterococci from Rabbits |
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| Authors: | M Simonová A Lauková |
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| Institution: | (1) Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovakia |
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| Abstract: | The antimicrobial spectrum of bacteriocin-producing enterococcal isolates from rabbits faeces, the biochemical characterization
of bacteriocins, and their molecular mass and the presence of structural genes for bacteriocin production were investigated.
Among enterococci selected from rabbit faeces, six strains of Enterococcus faecium (EF2019, EF1819, EF2119, EF1839, EF529, EF24/10) showed inhibitory activity against the indicators E. avium EA5, Listeria innocua LMG13568 and Listeria
monocytogenes CCM4699 and against other enterococci and staphylococci tested. The molecular mass of bacteriocin-like substances ranged
from 3 to 10 kDa. The presence of the structural genes for enterocins (ent) A, P and L50B was detected in all enterococci
tested. However, no strain possessed the gene for ent B. E.
faecium EF2019 showed the broadest inhibitory activity. A proteinaceous substance produced by the EF2019 strain was partially purified.
This is a thermostable substance that is stable at pH 4.0, 7.0 and 9.0. Its production starts in the early logarithmic growth
phase and culminates in the late logarithmic growth phase of the EF2019 strain. Partially purified bacteriocin (PPB) EF2019
added to the growing strain L. innocua LMG13568 (after 4 h) inhibited its growth as early as 1 h after addition with a decrease of 1.5 log cycles (5 h of cultivation).
This effect was extended up to 24 h. The bacteriocinogenic E. faecium EF2019 strain and/or bacteriocin EF2019 could be utilized against contaminant bacteria, e.g. in rabbitries. More detailed
studies under in vitro and in vivo conditions are in progress. |
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| Keywords: | antimicrobial activity bacteriocin characterization enterococci rabbit |
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