| 猪轮状病毒GD1株VP7基因的克隆及序列分析 |
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| 引用本文： | 田小艳,孙华,邓雨修,苏润环,王东东,宋延华.猪轮状病毒GD1株VP7基因的克隆及序列分析[J].广东畜牧兽医科技,2010,35(1):29-31. |
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| 作者姓名： | 田小艳 孙华 邓雨修 苏润环 王东东 宋延华 |
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| 作者单位： | 广东省温氏集团研究院,广东,新兴,527400 |
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| 摘 要： | 参考GenBank中发表的PRV OSU毒株VP7基因核苷酸序列ORF两端保守区序列,设计1对特异引物,以PRV GD1株反转录cDNA为模板,通过PCR方法扩增出长约1 kb的基因片段,并克隆到pMD18-T载体中进行序列测定。测序结果表明:VP7基因全长1 062 bp,含有一个981 bp的开放阅读框,编码326个氨基酸。与国内外已知的13个毒株VP7全长基因的核苷酸及推导的氨基酸序列比较,相似性分别为77.4%～100%和78.6%～99.7%;核苷酸系统发育进化树结果表明,GD1毒株与轮状病毒A2,JP3-6毒株亲缘关系较近,为一个进化群。
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| 关 键 词： | 猪轮状病毒 VP7基因 克隆 序列分析 |
Clone and sequence analysis of VP7 gene of porcine rotavirus strain GD1 |
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| Tian Xiaoyan,Sun Hua,Deng Yuxiu,Su Runhuan,Wang Dongdong,Song Yanhua.Clone and sequence analysis of VP7 gene of porcine rotavirus strain GD1[J].Guangdong Journal of Animal and Veterinary Science,2010,35(1):29-31. |
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| Authors: | Tian Xiaoyan Sun Hua Deng Yuxiu Su Runhuan Wang Dongdong Song Yanhua |
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| Institution: | Guangdong Win's Group Academy;Xinxing 527400;China |
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| Abstract: | A pair of primers targeted the conserved region flanking the ORF of VP7 gene of porcine rotavirus(PRV) strain OSU were designed according to the published sequences.The PCR product of approximately 1kb long was amplified from cDNA of PRV strain GD1.The PCR product was cloned into pMD18-T vector and sequenced.The full-length of VP7 gene was 1062 bp long including a coding region of 981bp,which encoded a protein with 326 amino acids.The VP7 gene of the PRV strain GD1 shared 77.4% to 100% necleotide homology w... |
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| Keywords: | Porcine rotaviru （PRV） VP7 gene clone sequence analysis |
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