Gene constitution of South-East Asian native chickens, commercial chickens and jungle fowl using polymorphisms of four calpain genes |
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| Authors: | Fumihiko OKUMURA Takeshi SHIMOGIRI Kotaro KAWABE Shin OKAMOTO Masahide NISHIBORI Yoshio YAMAMOTO Yoshizane MAEDA |
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| Institution: | Faculty of Agriculture, Kagoshima University, Kagoshima-shi, and;Graduate School of Biosphere Sciences, Department of Bioresource Science and Technology, Hiroshima University, Higashi-Hiroshima-shi, Japan |
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| Abstract: | The gene constitution of polymorphisms of the four calpain genes (µ‐calpain, m‐calpain, p94, and µ/m‐calpain) were analyzed in South‐East Asian native chickens, White Leghorn and Broiler commercial chickens, and Red and Green jungle fowl. Polymorphisms were detected at all loci in chickens and Red jungle fowl, but only for CAPN1 (µ‐calpain gene) in Green jungle fowl. CAPN2 and CAPN1.5 are linked on chicken chromosome 3, and the genotype for these loci were treated as haplotype. Some combinations of calpain loci were tested using principal component analysis, and the best combination (CAPN1, CAPN3, and CAPN1.5) was determined. The proportion of polymorphic loci (Ppoly) and heterozygosity (H?) were 1.00 and 0.316–0.465 in domestic chickens and red jungle fowl, and 0.33 and 0.137 in Green jungle fowl, respectively. GST values suggested that the degree of subdivision among native chickens was relatively low except for Thailand, which was highest. Pair‐wise FST testing, dendrogram and principal component analysis from the results of calpain loci showed that the four South‐East Asian native and commercial chicken populations were close genetically. |
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| Keywords: | calpain native chicken PCR-RFLP PCR-SSLP Southeast Asia |
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