| 患病与健康虾夷马粪海胆(Strongylocentrotus intermedius)体腔液菌群的PCR-DGGE分析比较 |
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| 引用本文： | 王轶南,刘艳萍,常亚青.患病与健康虾夷马粪海胆(Strongylocentrotus intermedius)体腔液菌群的PCR-DGGE分析比较[J].中国农业科技导报,2011,13(1):111-116. |
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| 作者姓名： | 王轶南 刘艳萍 常亚青 |
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| 作者单位： | 大连海洋大学,农业部海洋水产增养殖学重点开放实验室,辽宁大连,116023 |
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| 基金项目： | 国家高863计划项目(2006AA10A411);辽宁省重大科技攻关项目(2006203003);国家自然科学基金(309722697)资助。 |
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| 摘 要： | 分析比较患病与健康虾夷马粪海胆体腔液菌群的PCR-DGGE指纹图谱.并通过切胶、克隆测序等步骤分析患病虾夷马粪海胆体腔液中的特异菌群.结果显示,健康海胆体腔液中菌群的PCR-DGGE指纹图谱具7个条带,其中有5个与患病海胆相同,2个为不同条带,表明健康海胆体腔液中至少含7种细菌,其中2种以上细菌随机体环境变化而消亡;患...
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| 关 键 词： | 虾夷马粪海胆 体腔液 16S rDNA PCR-DGGE 疾病 |
| 收稿时间： | 2010-09-03 |
| 修稿时间： | 2010-09-27 |
Comparative Analysis of Bacterial Community Composition in Coelomic Fluid between Sick and Healthy Strongylocentrotus intermedius by PCR-DGGE |
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| WANG Yi-nan,LIU Yan-ping,CHANG Ya-qing.Comparative Analysis of Bacterial Community Composition in Coelomic Fluid between Sick and Healthy Strongylocentrotus intermedius by PCR-DGGE[J].Journal of Agricultural Science and Technology,2011,13(1):111-116. |
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| Authors: | WANG Yi-nan LIU Yan-ping CHANG Ya-qing |
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| Institution: | (Dalian Ocean University, Key Laboratory of Mari-culture, Ministry of Agriculture, Liaoning Dalian 116023, China) |
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| Abstract: | The bacterial community composition in the coelomic fluid of sick and healthy S. intermedius was analyzed using 16S rDNA PCR-DGGE fingerprint, and the special bacterium was analyzed by excising gel cloning and sequencing. The result indicated that 7 bands were observed in the PCR-DGGE fingerprint of coelomic fluid of healthy sea urchin, in which 5 bands were the same as the sick sea urchin, 2 were different bands. This suggested that there were at least 7 different bacteria in coelomic fluid of healthy sea urchin, and more than 2 bacteria disappeared with the internal environment change. 11 bands were found in sick sea urchin, including 6 special bands (Band 1~6) corresponding with each single bacterium, among which one was Sphingomonas sp., 3 were Vibrio sp., and the other 2 bands were not identified. Three vibrio strains had similarity with V. splendidus, V. tasmaniensis and V. cyclitrophicus, and were very likely to be the pathogen bacteria of S. intermedius. |
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| Keywords: | Strongylocentrotus intermedius coelomic fluid 16S rDNA PCR-DGGE disease |
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