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| 马传染性贫血病毒p26蛋白单克隆抗体的制备 | |
| 引用本文： | 韩秀娥,张萍,王雪峰,李萌,魏萍,周建华,尹杰超.马传染性贫血病毒p26蛋白单克隆抗体的制备[J].中国预防兽医学报,2011,33(7). |
| 作者姓名： | 韩秀娥 张萍 王雪峰 李萌 魏萍 周建华 尹杰超 |
| 作者单位： | 1. 东北农业大学 黑龙江省乳品工业技术开发中心,黑龙江哈尔滨,150030 2. 东北农业大学 动物医学院,黑龙江哈尔滨,150030 3. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/大动物病研究室,黑龙江哈尔滨,150001 4. 东北农业大学 生命科学院,黑龙江哈尔滨,150030 |
| 基金项目： | 国家自然科学基金，十一五重大传染病专项，省青年技术专项 |
| 摘 要： | 为制备马传染性贫血病毒(EIAV)的单克隆抗体(MAb),本研究以原核表达并纯化的EIAV p26蛋白作为免疫原免疫8周龄的BALB/c小鼠,利用淋巴细胞杂交瘤技术,建立抗EIAV衣壳蛋白p26抗体的杂交瘤细胞.应用重组p26蛋白为抗原建立ELISA筛选方法,获得两株能够稳定分泌特异性MAb的杂交瘤细胞株,分别命名为E11和F8.经试验证明,这些抗p26MAb均能够与感染驴胎皮肤细胞的EIAV和经SDS-PAGE分离的EIAV总蛋白中的p26蛋白结合.这两株p26蛋白MAb的获得将为EIAV的检测及鉴别诊断奠定基础. |
| 关 键 词： | 马传染性贫血病毒 p26蛋白 单克隆抗体 |
Preparation of monoclonal antibody to nucleocapsid protein of equine infectious anemia virus |
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| HAN Xiu-e,ZHANG Ping,WANG Xue-feng,LI Meng,WEI Ping,ZHOU Jian-hua,YIN Jie-chao.Preparation of monoclonal antibody to nucleocapsid protein of equine infectious anemia virus[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(7). | |
| Authors: | HAN Xiu-e ZHANG Ping WANG Xue-feng LI Meng WEI Ping ZHOU Jian-hua YIN Jie-chao |
| Institution: | HAN Xiu-e1#,ZHANG Ping2#,WANG Xue-feng3,LI Meng1,WEI Ping2,ZHOU Jian-hua3,YIN Jie-chao4 (1. Heilongjiang Dairy Industry Technical Development Center,Northeast Agricultural University,Harbin 150030,China,2. College of Veterinary Medicine,3. State Key Laboratory of Veterinary Biotechnology,Division of Livestock Diseases,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,4. College of Life S... |
| Abstract: | To prepare the monoclonal antibody(MAb) against equine infectious anemia virus (EIAV), Eight-week-old female BALB/c mice were immunized with purified recombinant p26 protein. Two hybridoma cell lines of E11 and F8 were produced by using hybridoma technique and the MAbs were identifined and analyzed by indirect ELISA and western blot. The results showed that the MAbs were high specificity to both recombinant p26 and donkey leucocyte attenuated EIAV. |
| Keywords: | EIAV p26 Protein monoclonal antibody |
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