| 抑制大肠杆菌K88的益生菌体外筛选 |
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| 引用本文： | 王磊,吕阳,张越,谢佳倩,赵迪,晏利琼,梅慧敏,王蕾,易丹,陈洪波,丁斌鹰,侯永清,吴涛.抑制大肠杆菌K88的益生菌体外筛选[J].中国畜牧兽医,2016,43(5):1355-1360. |
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| 作者姓名： | 王磊 吕阳 张越 谢佳倩 赵迪 晏利琼 梅慧敏 王蕾 易丹 陈洪波 丁斌鹰 侯永清 吴涛 |
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| 作者单位： | 1. 武汉轻工大学动物科学与营养工程学院, 武汉 430023;2. 动物营养与饲料科学湖北省重点实验室, 武汉 430023 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(31302089);湖北省自然科学基金项目(2012FFBO4804) |
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| 摘 要： | 本研究以从断奶仔猪肠道分离的益生菌为试验菌株,将大肠杆菌K88和益生菌接种到体外培养的猪小肠上皮细胞(intestinal porcine epithelial cell-1,IPEC-1)中,测定培养2.5h上清液中的乳酸脱氢酶(lactate dehydrogenase,LDH)活性,同时将益生菌和大肠杆菌K88体外混合培养2.5h,进行平板计数,统计大肠杆菌K88菌数的变化,筛选出可以抑制大肠杆菌K88的益生菌。试验结果显示,干酪乳杆菌和凝结芽孢杆菌能显著降低IPEC-1培养上清液中的LDH活性(P<0.05),降低大肠杆菌K88对细胞的损伤;凝结芽孢杆菌能降低DMEM培养基中大肠杆菌K88的生长速度,结合模拟制粒过程及胃肠道环境的耐高温、耐酸及耐胆盐研究进行综合分析,该凝结芽胞杆菌对大肠杆菌K88有较好的抑制作用且具有良好的耐高温、耐酸及耐胆盐性能,具有作为微生态制剂菌株的应用潜力。本试验建立了能够抑制大肠杆菌K88的益生菌体外筛选技术模型。
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| 关 键 词： | 益生菌 干酪乳杆菌 凝结芽孢杆菌 IPEC-1细胞系 LDH活性 平板计数法 |
| 收稿时间： | 2015-11-24 |
Screening of Probiotics Inhibiting E. coli K88 in vitro |
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| WANG Lei,LV Yang,ZHANG Yue,XIE Jia-qian,ZHAO Di,YAN Li-qiong,MEI Hui-min,WANG Lei,YI Dan,CHEN Hong-bo,DING Bin-ying,HOU Yong-qing,WU Tao.Screening of Probiotics Inhibiting E. coli K88 in vitro[J].China Animal Husbandry & Veterinary Medicine,2016,43(5):1355-1360. |
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| Authors: | WANG Lei LV Yang ZHANG Yue XIE Jia-qian ZHAO Di YAN Li-qiong MEI Hui-min WANG Lei YI Dan CHEN Hong-bo DING Bin-ying HOU Yong-qing WU Tao |
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| Institution: | 1. School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China;2. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan 430023, China |
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| Abstract: | The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro. |
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| Keywords: | probiotics Lactobacillus casei Bacillus coagulans IPEC-1 cell line LDH activity plate count method |
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