| 猪粪中高温纤维素分解菌的分离、筛选和鉴定 |
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| 引用本文： | 郭艳,张进良.猪粪中高温纤维素分解菌的分离、筛选和鉴定[J].农业科学与技术,2010(5). |
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| 作者姓名： | 郭艳 张进良 |
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| 作者单位： | 商丘师范学院生命科学系,河南商丘,476000 |
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| 基金项目： | 河南省教育厅自然基金资助项目(2010B530001).Supported by Foundation of Henan Educational Committee |
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| 摘 要： | 目的]为研制高效生物降解菌剂奠定基础.方法]在60℃条件下对猪粪中纤维素分解菌进行了分离研究,对分离到的菌株进行羧甲基纤维素酶活(CMCase)和滤纸酶活(FPase)测定,并进行16S rRNA基因分析.结果]BC1和BC3号菌株CMCase、Fpase都较高,但相差不大,通过16S rDNA序列分析发现BC1和BC3号菌株分别与Pseudomonas sp.的相似性达到98%和99%.结论]该研究为研制高效生物增温剂奠定了基础.
Abstract：
Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums.Method]under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. Result] The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp.(98% and 99% similarities,respectively).Coaclusion]the experiment laid foundation for high-efficiency biological heating agent.
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| 关 键 词： | 猪粪 中高温 纤维素分解菌 分离研究 筛选 鉴定 Pig carboxymethyl cellulose Pseudomonas 16S rRNA 16S rDNA enzyme activity 菌株 filter paper sequence of 纤维素酶活 生物增温剂 experiment difference 研制 |
Isolation,Screening and Identification of High-temperature Cellulolytic Microbes in Pig Manure |
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| Abstract: | Pig manure High-temperature cellulolytic microbes 16S rRNA gene |
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| Keywords: | Pig manure High-temperature cellulolytic microbes 16S rRNA gene |
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