| 一株烤烟根际解磷细菌的鉴定及其在烤烟生产中的应用 |
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| 引用本文： | 李晓举,吴风光,王豹祥,席淑雅,张朝辉,邱立友.一株烤烟根际解磷细菌的鉴定及其在烤烟生产中的应用[J].河南农业科学,2011,40(6):66-70. |
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| 作者姓名： | 李晓举 吴风光 王豹祥 席淑雅 张朝辉 邱立友 |
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| 作者单位： | 1. 河南农业大学生命科学学院,河南郑州,450002
2. 湖北中烟工业有限责任公司,湖北武汉,430051 |
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| 基金项目： | 湖北中烟工业有限责任公司项目 |
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| 摘 要： | 对分离自烤烟根际的解磷菌P04进行鉴定,并通过小区试验探讨其在烤烟生产中的作用.应用16S rDNA序列构建系统发育树,结合生理指标、生化反应,对解磷菌P04进行鉴定,结果表明,该菌株属于肠杆菌属(Enterobacter),初步鉴定为阴沟肠杆菌(Enterobacter cloacae).将解磷菌P04制作菌肥(代号...
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| 关 键 词： | 烤烟 解磷菌 16S rDNA 阴沟肠杆菌 |
Identification and Application of a Phosphate Solubilizing Bacterium from Flue-cured Tobacco Rhizosphere |
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| LI Xiao-ju,WU Feng-guang,WANG Bao-xiang,XI Shu-ya,ZHANG Chao-hui,QIU Li-you.Identification and Application of a Phosphate Solubilizing Bacterium from Flue-cured Tobacco Rhizosphere[J].Journal of Henan Agricultural Sciences,2011,40(6):66-70. |
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| Authors: | LI Xiao-ju WU Feng-guang WANG Bao-xiang XI Shu-ya ZHANG Chao-hui QIU Li-you |
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| Institution: | 1(1.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China;2.China Tobacco Hubei Industrial Co.,Ltd.,Wuhan 430051,China) |
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| Abstract: | Phosphate solubilizing bacteria P04 isolated from flue-cured tobacco rhizosphere was identified,and applied to reduce dosage of chemical P fertilizer in flue-cured tobacco production.Phosphate solubilizing bacteria P04 was identified as Enterobacter sp.,maybe Enterobacter cloacae by phylogenetic tree based on 16S rDNA sequences,the physiological and biochemical reaction.Phosphate solubilizing bacteria fertilizer prepared using P04 was used to flue-cured tobacco production.In this study,phosphate solubilizing bacteria fertilizer was employed by 30kg/ha when flue-cured tobacco transplanted in plots with minus 20% P dosage of full fertilizer.Results showed that employing phosphate solubilizing bacteria fertilizer increased the average amount of phosphate solubilizing bacteria in tobacco rhizosphere by 28.19%.The average contents of P in tobacco leaves at different growth stages significantly increased by 19.13%.Accordingly the yield and production value with phosphate solubilizing bacteria fertilizer were slightly improved compared to control.The results suggested that phosphate solubilizing bacteria fertilizer allowed reduced application rates of chemical P fertilizers.To our knowledge,this is the first report on phosphate solubilizing bacteria Enterobacter cloacae in flue-cured tobacco rhizosphere and employing of it could enhance significantly the account of phosphate solubilizing bacteria in flue-cured tobacco rhizosphere and the yield and quality of tobacco leaves. |
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| Keywords: | Flue-cured tobacco Phosphate solubilizing bacteria 16S rDNA Enterobacter cloacae |
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