| 番茄叶霉病拮抗链霉菌BPS2的筛选及鉴定 |
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| 引用本文： | 曹广丽,陈立梅,徐文静,董英山,李启云.番茄叶霉病拮抗链霉菌BPS2的筛选及鉴定[J].东北农业大学学报,2007,38(3):343-347. |
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| 作者姓名： | 曹广丽 陈立梅 徐文静 董英山 李启云 |
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| 作者单位： | 吉林省农业科学院,吉林,公主岭,136100;东北农业大学资源与环境学院,哈尔滨,150030;吉林省农业科学院,吉林,公主岭,136100;吉林农业大学农学院,长春,130118;吉林省农业科学院,吉林,公主岭,136100 |
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| 基金项目： | 吉林省农业科学院基金，国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 从江苏昆山、吉林图门等地采集的土壤样品中分离到1株对番茄叶霉病菌有强烈抑制作用的BPS2菌株。研究其拮抗性表明,对革兰氏阳性细菌、革兰氏阴性细菌以及多种病原真菌均有强烈的抑制作用。根据形态特征、培养特征、生理生化特性及16S rDNA序列等方面的多项分类研究,确定其属于链霉菌属,并被命名为Streptomyces tumenensis BPS2。该菌株在大多数培养基上生长良好,基内菌丝无横隔,不断裂,气生菌丝体形成孢子丝,孢子丝呈波曲或直形。
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| 关 键 词： | 番茄叶霉病 16S rDNA 链霉菌 |
| 文章编号： | 1005-9369（2007）03-0343-05 |
| 修稿时间： | 2006-01-04 |
Isolation and identification of Streptomyces tumenensis BPS2 antagonistic to tomato leaf mould |
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| CAO Guangli,CHEN Limei,XU Wenjing,DONG Yingshan,LI Qiyun.Isolation and identification of Streptomyces tumenensis BPS2 antagonistic to tomato leaf mould[J].Journal of Northeast Agricultural University,2007,38(3):343-347. |
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| Authors: | CAO Guangli CHEN Limei XU Wenjing DONG Yingshan LI Qiyun |
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| Institution: | 1. Jilin Academy of Agricultural Sciences, Gongzhuling Jilin 136100, China; 2. Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China; 3. Agricultural College, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Streptomyces tumenensis BPS2 was isolated from soil samples collected from different areas such as Kunshan, Tumen which was antagonistic to tomato leaf mould. It had distinctively inhibitive effect on gram-positive baterium,gram-negative bacterium and some kinds of pathogenic fungi. Based on morphological, cultural,physio-biochemical characteristics and 16S rDNA sequence analysis, it was proposed as Stretomyces and was designated as Streptomyces tumenensis BPS2. This strain grew well on most of the tested culture media. The substrate mycelia have no branches, the aerial mycelia produce the spore chain and the spore chain are curved or straight. |
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| Keywords: | 16S rDNA |
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