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| 白头翁汤及其主要成分对LPS诱导内皮细胞分泌NO、E-selectin和IL-8的影响 | |
| 引用本文： | 胡屹屹,穆祥,胡元亮.白头翁汤及其主要成分对LPS诱导内皮细胞分泌NO、E-selectin和IL-8的影响[J].畜牧兽医学报,2012,43(1):145-151. |
| 作者姓名： | 胡屹屹 穆祥 胡元亮 |
| 作者单位： | 1. 江苏省农业科学院兽医研究所,南京,210014 2. 北京农学院兽医学中医药北京市重点实验室,北京,102206 3. 南京农业大学动物医学院,南京,210095 |
| 摘 要： | 拟探讨白头翁汤及其主要成分对细菌内毒素(LPS)导致的机体炎症等病理损伤的治疗作用.培养内皮细胞至单层融合状态,加入内毒素刺激,3h后加入高、中、低3种浓度的8种药物,继续培养21 h后收集细胞上清液,用ELISA方法测定NO、E-selectin和IL-8的含量.结果显示,白头翁汤高剂量组、白头翁素高剂量组和小檗碱高剂量组的NO含量极显著低于LPS对照组(P＜0.01),白头翁汤中剂量组、白头翁素中剂量组、小檗碱中剂量组和秦皮乙素高剂量组显著低于LPS对照组(P＜0.05)；白头翁汤高剂量组和药根碱高剂量组的E-selectin含量极显著低于LPS对照组(P＜0.01),白头翁皂苷B4高剂量组、小檗碱高剂量组和药根碱中剂量组显著低于LPS对照组(P＜0.05)；秦皮乙素高剂量组的IL-8含量极显著低于LPS对照组(P＜0.01),白头翁汤高剂量组、秦皮甲素高剂量组和中剂量组、秦皮乙素中剂量组显著低于LPS对照组(P＜0.05).结果提示白头翁汤及其主要成分具有明显的抗细菌内毒素作用,可在由NO、E-selectin和IL-8介导的机体炎症等病理损伤过程中发挥治疗作用. |
| 关 键 词： | 白头翁汤 内毒素 一氧化氮 E-选择素 白介素-8 |
Effects of Pulsatillae Decoction and Its Main Ingredients on LPS-induced NO,E-selectin and IL-8 Secretion of Endothelial Cells |
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| HU Yi-yi , MU Xiang , HU Yuan-liang.Effects of Pulsatillae Decoction and Its Main Ingredients on LPS-induced NO,E-selectin and IL-8 Secretion of Endothelial Cells[J].Acta Veterinaria et Zootechnica Sinica,2012,43(1):145-151. | |
| Authors: | HU Yi-yi MU Xiang HU Yuan-liang |
| Institution: | 1.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China;2.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture,Beijing 102206,China;3.College of Veterinary Medicine, Nanjing Agricultural University,Nanjing 210095,China) |
| Abstract: | The aim of the research was to investigate the antiendotoxin of Pulsatillae Decoction(PD),the levels of NO,E-selectin and IL-8 secreted by cultured rat intestinal microvascular endothelial cells were determined after treatment with its active ingredients,namely anemoside B4,anemonin,berberine,jatrorrhizine,palmatine,aesculin,and esculetin.Endothelial cell was challenged with 1 μg·mL-1 LPS for 3 h,then treated respectively with PD at three concentrations(1,5 and 10 mg·mL-1) and its seven ingredients at three concentrations(1,5 and 10 μg·mL-1).The cells were incubated at 37 ℃ in a cell incubator for 21 hours.The supernatants were collected and the content of NO,E-selectin and IL-8 were analyzed by ELISA kits.The results showed that as compared with LPS control group,the NO contents were significantly decreased in the middle-and high-dose groups of PD,the middle and high-dose groups of Anemonin,the middle-and high-dose groups of Berberine and the high-dose group of Esculetin(P<0.01 or P<0.05);The E-selectin contents were significantly decreased in the high-dose group of PD,the high-dose group of Anemoside B4,the high-dose group of Berberine and the middle-and high-dose groups of Jateorhizine(P<0.01 or P<0.05);The IL-8 contents were significantly decreased in high-dose group of PD,the middle-and high-dose groups of Aesculin and the middle-and high-dose groups of Esculetin(P<0.01 or P<0.05).It suggested that PD may exert beneficial therapeutic actions on systemic inflammatory response syndrome,multiple organ dysfunction syndrome and immune diseases mediated by NO,E-selectin and IL-8. |
| Keywords: | Pulsatillae Decoction LPS NO E-selectin IL-8 |
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