| PCV2感染对仔猪NO-NOS系统的动态影响 |
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| 引用本文： | 陈耿,刘浩飞,王兆飞,张亚群,陈鸿娟,郭昊,张书霞.PCV2感染对仔猪NO-NOS系统的动态影响[J].中国兽医学报,2011,31(4). |
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| 作者姓名： | 陈耿 刘浩飞 王兆飞 张亚群 陈鸿娟 郭昊 张书霞 |
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| 作者单位： | 南京农业大学动物医学院,江苏南京,210095 |
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| 基金项目： | 国家自然科学基金资助项目(30871848) |
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| 摘 要： | 将19头PCV2和PRRSV血清抗体阴性的普通断奶仔猪分为对照组(4头)和攻毒组(15头),攻毒组每头仔猪滴鼻接种PCV2病毒悬液4mL(5×105 TCID50/mL)并用KLH刺激,分别在攻毒后14、21、35d各扑杀5头仔猪采集血清和脾脏、淋巴结、胸腺(对照组4头在攻毒当天扑杀)。检测各种组织中NO、TNOS和iNOS的含量。结果显示,攻毒猪各组织中NO含量均在攻毒后14d显著升高(P<0.05),然后缓慢下降;血清和3种免疫器官中NOS(包括TNOS和iNOS)活性的变化趋势比较一致,均表现出先升高然后再逐渐降低的特点。此外,iNOS活性变化与其相应的组织中NO含量呈显著正相关(P<0.01)。结果表明,PCV2在感染早期(1～14d)可激活仔猪多种组织中NO-NOS系统,中后期逐渐恢复,这种变化与PCV2所致的组织损伤密切相关,因此,NO-NOS系统可能是PCV2相关疾病发生发展的重要信号。
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| 关 键 词： | 猪圆环病毒Ⅱ型 仔猪 一氧化氮 一氧化氮合酶 |
Dynamic effect of PCV2 infection on NO-NOS system of piglets |
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| CHEN Geng,LIU Hao-fei,WANG Zhao-fei,ZHANG Ya-qun,CHEN Hong-juan,GUO Hao,ZHANG Shu-xia.Dynamic effect of PCV2 infection on NO-NOS system of piglets[J].Chinese Journal of Veterinary Science,2011,31(4). |
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| Authors: | CHEN Geng LIU Hao-fei WANG Zhao-fei ZHANG Ya-qun CHEN Hong-juan GUO Hao ZHANG Shu-xia |
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| Institution: | CHEN Geng,LIU Hao-fei,WANG Zhao-fei,ZHANG Ya-qun,CHEN Hong-juan,GUO Hao,ZHANG Shu-xia*(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | The interaction between porcine circovirus type 2(PCV2) and the host immune system has been suggested to be a determinant event for the pathogenesis of post-weaning multisystemic wasting syndrome(PMWS).To gain insight into the mechanism of immune suppression caused by PCV2 in piglets,nineteen conventional post-weaning piglets without PCV2 and PRRSV antibody were randomly distributed in two groups: the control group(n=4),the experiment group(n=15),Every piglet in experiment group was inoculated with PCV2 and... |
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