| 副猪嗜血杆菌tbpA基因PCR—RFLP分型 |
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| 引用本文： | 李军星,姜平,王艳,李玉峰,陈闻,王先玮,李鹏.副猪嗜血杆菌tbpA基因PCR—RFLP分型[J].中国兽医寄生虫病,2009,17(1):45-50. |
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| 作者姓名： | 李军星 姜平 王艳 李玉峰 陈闻 王先玮 李鹏 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京,210095 |
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| 基金项目： | 浙江省重点科研农业项目，国家科技支撑计划子项目 |
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| 摘 要： | 副猪嗜血杆菌（Haemophilus parasuis，HPs）是目前影响世界养猪业的重要病原之一。本试验利用针对HPs转铁蛋白基因tbpA的PCR—RFLP分型方法，对2003-2008年分离自江苏、上海、广西、浙江、江西和安徽等6省市的57个HPs分离株及15个参考菌株进行PCR—RFLP分析。结果显示，15个血清型参考菌株分为9种基因型，57个HPs流行分离株分为15种基因型，其中在我国最为流行的基因型分别为DBN（38％），ABN（18％）与DBP（12％）。本研究表明副猪嗜血杆菌在我国猪群中普遍存在，并至少有15个RFLP基因亚型，从而为我国HPs病的防治提供了重要理论依据。
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| 关 键 词： | 副猪嗜血杆菌 tbpA基因 PCR-RFLP 基因型 |
CHARACTERIZATION OF HAEMOPHILUS PARASUIS FIELD ISOLATES FROM CHINA BY PCR-RFLP ANALYSIS OF THE TBPA GENE |
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| LI Jun-xing,JIANG Ping,WANG Yan,LI Yu-feng,CHEN Wen,WANG Xian-wei,LI Peng.CHARACTERIZATION OF HAEMOPHILUS PARASUIS FIELD ISOLATES FROM CHINA BY PCR-RFLP ANALYSIS OF THE TBPA GENE[J].Chinese Journal of Veterinary Parasitology,2009,17(1):45-50. |
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| Authors: | LI Jun-xing JIANG Ping WANG Yan LI Yu-feng CHEN Wen WANG Xian-wei LI Peng |
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| Institution: | (Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China) |
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| Abstract: | To study the genotype of Haemophilus parasuis and its distribution in China,57 Hearnophilus parasuis isolates along with 15 reference strains of all known serovars were subjected to be analyzed using PCR- FRLP （restriction fragment length polymorphism） based on tbpA gene. The analysis of the 1.9 - kb tbpA amplicon using Taq I, Ava I and Afa I endonucleases showed 9 RFLP patterns of the15 reference strains and 15 patterns of the 57 field isolates. Moreover, the first 3 genotypes of most prevalence in China were DBN （38%）, ABN （18%） and DBP （12%）. Our results confirmed that Haemophilus parasuis existed widely in China with numerous genotypes and also provided significant basis for the prevention and control of the disease in China. |
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| Keywords: | PCR-RFLP |
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