Characterization of the receptor for gonadotropin-releasing hormone in the pituitary of the African catfish,Clarias gariepinus |
| |
| Authors: | R De Leeuw P M Conn C Van't Veer H J Th Goos P G W J Van Oordt |
| |
| Institution: | (1) Department of Experimental Zoology, Research group for Comparative Endocrinology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands;(2) Department of Pharmacology, University of Iowa, College of Medicine, 52242 Iowa City, Iowa, USA |
| |
| Abstract: | Receptors for gonadotropin-releasing hormone (GnRH) were characterized using a radioligand prepared from a superactive analog of salmon GnRH (sGnRH), D-Arg6-Pro9-sGnRH-NEt (sGnRHa). Binding of125I-sGnRHa to catfish pituitary membrane fractions reached equilibrium after 2 h incubation at 4°C. Displacement experiments with several GnRH analogs as well as other peptides, demonstrated the specificity of125I-sGnRHa binding. Specific binding was enhanced in the presence of the cation chelator ethylene bis (oxyethylenenitrilo) tetra-acetic acid (EGTA), indicating an inhibitory effect of cations on GnRH-receptor binding. The binding of125I-sGnRHa to pituitary membranes was found to be saturable at radioligand concentrations of 5 nM and above. A Scatchard analysis of the saturation data suggested the presence of a single class of high-affinity binding sites (Ka=0.901±0.06×109M–1, Bmax=1678±150 fmol/mg protein). A comparative study on125I-sGnRHa binding to pituitary membrane fractions of male and female catfish, indicated that there were no differences in binding affinity and binding capacity between both sexes. The results demonstrate the presence of specific, saturable GnRH receptors in the African catfish pituitary. |
| |
| Keywords: | catfish pituitary GnRH receptor |
| 本文献已被 SpringerLink 等数据库收录！ |
|
