| 山羊Callipyge基因型的检测 |
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| 引用本文： | 王海亮,李祥龙,周荣艳,李兰会,郭秀丽.山羊Callipyge基因型的检测[J].中国畜牧兽医,2007,34(11):49-51. |
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| 作者姓名： | 王海亮 李祥龙 周荣艳 李兰会 郭秀丽 |
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| 作者单位： | 河北农业大学动物科技学院，保定 071000 |
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| 摘 要： | 本试验扩增了波尔山羊、南江黄羊和承德无角山羊3个品种113个个体的Callipyge基因长度为493 bp的片段。 经测序，发现一个SNP位点，第184 bp处C→T。对Callipyge限制性酶切位点（Fok I）的PCR-RFLP的多态性进行分析，在波尔山羊和南江黄羊两个品种中表现多态性，得到CC、CT和TT 3种基因型，波尔山羊群体中基因型频率分别为0.6571、0.3143、0.0286，南江黄羊群体中基因型频率分别为0.8947、0.1053、0.0000，而承德无角山羊全部为CC基因型。研究结果提示184C→T可能与山羊双肌性状有关。
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| 关 键 词： | Callipyge基因 基因型 SNP PCR-RFLP |
| 文章编号： | 1671-7236（2007）11-0049-03 |
| 修稿时间： | 2007-04-19 |
Detection of Goat Callipyge Genotype |
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| WANG Hai-liang,LI Xiang-long,ZHOU Rong-yan,LI Lan-hui,GUO Xiu-li.Detection of Goat Callipyge Genotype[J].China Animal Husbandry & Veterinary Medicine,2007,34(11):49-51. |
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| Authors: | WANG Hai-liang LI Xiang-long ZHOU Rong-yan LI Lan-hui GUO Xiu-li |
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| Institution: | Agricultural University of Hebei,Baoding 071000,China |
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| Abstract: | The 493bp fragment of Callipyge gene of 113 individuals was amplified by PCR,of which 35 were from Boer goat,38 were from Nanjiang brown goat and 40 were from Chengde polled goat.There was a variation of C184T discovered by sequencing.Molecular genetic diversity of Callipyge gene with restriction endomuclease Fok I was studied by PCR-RFLP.The polymorphism was existed in Boer goat and Nanjiang brown goat.There were 3 genotypes,which was CC,CT and TT,respectively.The frequency of genotypes was 0.6571,0.3143 and 0.0286 respectively in Boer goat.The frequency of genotypes was 0.8947,0.1053 and 0.0000 respectively in Nanjiang brown goat.However,there is all CC genotype in Nanjiang brown goat.It suggested that there was a relationship of the variation of C184T to the trait of Callipyge in goat. |
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| Keywords: | SNP PCR-RFLP |
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