| 葡萄糖氧化酶纳米粒的制备及其体内外抗菌效果 |
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| 引用本文： | 周名清,程天印,张嘉恒.葡萄糖氧化酶纳米粒的制备及其体内外抗菌效果[J].畜牧与兽医,2022(2). |
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| 作者姓名： | 周名清 程天印 张嘉恒 |
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| 作者单位： | 湖南农业大学动物医学院;哈尔滨工业大学（深圳）材料科学与工程学院 |
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| 基金项目： | 国家自然科学基金(21905069)。 |
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| 摘 要： | 为了使葡萄糖氧化酶更加稳定,利用可逆加成-断裂链转移自由基聚合反应对葡萄糖氧化酶进行纳米包裹。动态激光散射仪测定显示,包裹后粒径为(12.4±1.0)nm, Zeta电位为(+4.2±0.5)mV。通过冻融循环、长期储存和胰蛋白酶试验证明了包裹后的稳定性。在体外,对大肠杆菌的最低抗菌浓度为0.01 mg/mL。对新西兰大白兔细菌肿包治疗显示,包裹后的葡萄糖氧化酶较包裹前有较好的稳定性和抑菌效果,可作为新型抗菌制剂。
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| 关 键 词： | 葡萄糖氧化酶 纳米包裹 稳定性 抗菌 |
Preparation of glucose oxidase nanoparticles and their antimicrobial effects in vitro and in vivo |
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| ZHOU Mingqing,CHENG Tianyin,ZHANG Jiaheng.Preparation of glucose oxidase nanoparticles and their antimicrobial effects in vitro and in vivo[J].Animal Husbandry & Veterinary Medicine,2022(2). |
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| Authors: | ZHOU Mingqing CHENG Tianyin ZHANG Jiaheng |
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| Institution: | (College of Veterinary Medicine,Hunan Agricultural University,Changsha 410000,China;College of Materials Science and Engineering,Harbin Institute of Technology(at Shenzhen),Shenzhen 518055,China) |
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| Abstract: | In order to make glucose oxidase more stable, glucose oxidase was nano-coated by reversible addition-fragmentation chain transfer reaction. The particle size after coating was(12.4±1.0)nm and the Zeta potential was(+4.2±0.5)mV, as determined by dynamic laser scattering. The stability of glucose oxidase after coating was demonstrated by freeze-thaw cycles, long-term storage and trypsin tests. In vitro, the lowest antibacterial concentration of the coated glucose oxidase against E.coli was 0.01 mg/mL. The treatment of New Zealand rabbits with bacterial swelling showed that the coated glucose oxidase had higher stability and better antibacterial effect than before coating. The present study suggested that nano-coated glucose oxidase could be expected as a new antibacterial agent. |
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| Keywords: | glucose oxidase nano-coated stability anti-bacteria |
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