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| 长江下游4属6种鳞科鱼类线粒体16S rDNA部分序列的比较分析 | |
| 引用本文： | 秦钦,蔡永祥,许志强,葛家春,陈校辉,霍光明,边文冀.长江下游4属6种鳞科鱼类线粒体16S rDNA部分序列的比较分析[J].江苏农业学报,2010,26(2). |
| 作者姓名： | 秦钦 蔡永祥 许志强 葛家春 陈校辉 霍光明 边文冀 |
| 作者单位： | 1. 江苏省淡水水产研究所,江苏,南京,210017;江苏省野生水生动物移植驯化及苗种繁育中心,江苏,南京,210017 2. 南京晓庄学院生命科学系,江苏,南京,211171 3. 江苏省淡水水产研究所,江苏,南京,210017 |
| 基金项目： | 江苏省三项工程项目(K2007-8、BZ2008-7);;“十一五”国家科技支撑计划子专题(2006BAD03B07-04) |
| 摘 要： | 采用PCR扩增获得了长江下游鲿科(Bagridae)4个属6个种类的线粒体16S rDNA序列片段。序列长度介于920 bp与933 bp之间,A+T含量明显高于C+G含量。共有939个比对位点,其中190个为变异位点;52个为简约信息位点。以大鳞脂鲤(Chalceus macrolepidotus)为外群,采用邻接法(NJ)和最大简约法(MP)分析鲿科4属6种鱼类的系统发生关系。结果表明,16S rDNA序列可以作为鲿科属间系统发育分析的有效分子标记。聚类结果显示,鲿科鱼类构成一个单系类群,其中鳠属(Mystus)较特化,黄颡鱼属(Pelteobagrus)次之,而拟鲿属(Pseud-obagrus)与鮠属(Leiocassis)的亲缘关系最近。 |
| 关 键 词： | 鲿科 16S rDNA 分子系统学 |
Comparison of Six Species of Four Genera of Bagridae in the Lower Yangtze River Based on 16S rDNA Gene Sequences |
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| QIN Qin,CAI Yong-xiang,XU Zhi-qiang,GE Jia-chun,CHEN Xiao-hui,HUO Guang-ming,BIAN Wen-ji.Comparison of Six Species of Four Genera of Bagridae in the Lower Yangtze River Based on 16S rDNA Gene Sequences[J].Jiangsu Journal of Agricultural Sciences,2010,26(2). | |
| Authors: | QIN Qin CAI Yong-xiang XU Zhi-qiang GE Jia-chun CHEN Xiao-hui HUO Guang-ming BIAN Wen-ji |
| Institution: | 1.Freshwater Fisheries Research Institute of Jiangsu Province/a>;Nanjing 210017/a>;China/a>;2.Jiangsu Engineering Research Center for Wild Aquatic Animals Introduction & Hatching/a>;3.School of Life Science/a>;Nanjing Xiaozhuang Normal University/a>;Nanjing 211171/a>;China |
| Abstract: | The 16S rDNA sequences of six species from four genera of Bagridae in the lower Yangtze River were amplified,ranging from 920 bp to 933 bp.The average base composition is A: 35.2% T: 22.5% C: 23.1% G: 19.2%,showing that the A+T content is higher than that of C+G.The alignment length of the 16S rDNA sequences was 939 sites,including 190 variable and 52 parsimony informative sites.Choosing Chalceus macrolepidotus as the outgroup,the phylogenetic trees were constructed with MEGA4.0 by the neighbor-joining meth... |
| Keywords: | Bagridae 16S ribosomal DNA molecular phylogenetics |
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