Cloning and Bioinformatics Analysis of espP Gene of Haemophilus parasuis |
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| Authors: | YOU Jin-zhou ZHANG Rui-zhen QIU Jian-long XIE Min HU Ling-ying |
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| Institution: | 1. Longyan Entry-exit Inspection & Quarantine Bureau, Longyan 364000, China;2. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | In order to evaluate whether Haemophilus parasuis espP gene could use as a vaccine candidate antigen or not,the espP gene was amplified by PCR using the specific primers that designed according to corresponding sequence getting form GenBank (accession No.:HAPS_1381) and then sent to sequence.After translating the DNA sequence into amino acid sequence,the signal peptide,hydrophilic region,linear B cell epitopes and 3D structure of Hps espP protein were predicted using multiple bioinformatics analysis.The results showed that the full length of Hps espP gene was 2 343 bp,encoding 781 aa.The first 23 aa were predicted as signal peptide.There were 12 hydrophilic regions and 12 B cell epitopes in the Hps espP protein.The 3D structure of C-terminal of the protein was consisted of a monomeric β-barrel containing 12 β-strands and a central pore.The study laid the foundation for further development of molecular vaccines based on espP protein against Hps infection. |
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| Keywords: | Haemophilus parasuis espP clone bioinformatics |
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